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The map label for this gene is btuF [H]

Identifier: 194737235

GI number: 194737235

Start: 240499

End: 241287

Strand: Reverse

Name: btuF [H]

Synonym: SeSA_A0229

Alternate gene names: 194737235

Gene position: 241287-240499 (Counterclockwise)

Preceding gene: 194736622

Following gene: 194737595

Centisome position: 5.12

GC content: 55.64

Gene sequence:

>789_bases
ATGTTCAGGGCGCTGGTCGCCCTGCTGCTTACGCTCCCGGTGTGGCTTTACGCTGCGCCGCGCGTCATTACCCTCTCTCC
CGCCAATACGGAGCTCGCTTTTGCCGCCGGGATTACGCCCGTTGGCGTCAGCAGCTATTCCGACTACCCGCCCGAAGCGC
AAAAAATAGAACAGGTCTCTACCTGGCAGGGAATGAATCTGGAACGCATTGTGGCGCTGAAGCCGGATCTGGTCGTCGCC
TGGCGCGGCGGTAATGCGGAACGCCAGGTAAACCAACTGACGTCATTAGGTATTAAGGTCATATGGGTGGACGCGGTAAG
CATCGAACAGATCGCCGACACGCTCCGTCAACTGGCCGCCTGGAGCCCGCAGCCGGAAAAGGCTCAGCAGGCAGCGCAGA
CATTGCTAAACGAGTACGCCGCGCTCAACGCCGAGTATGCCGGTAAAGCTAAAAAGCGCGTCTTTCTTCAGTTTGGCATG
AATCCTCTGTTTACCAGTGGTAAAGGGTCTATTCAACATCAGGTTTTGACGACCTGCGGCGGAGAAAACGTCTTTGCTGA
CAGCCGCGTGCCGTGGCCGCAAGTCAGCCGTGAACAGGTGCTGGCAAGGCATCCTCAGGCCATTATCGTGGCCGGAAAAG
CGGGCGAAATTCTCAAAATTGAACAATACTGGGGAAACCTGTTAAAAATTCCGGTTATTCCGCTTAACAGCGACTGGTTT
GAACGCGCAAGCCCGCGTATTATCCTCGCCGCAAAACAACTCTGTAATGCGCTTTCACAGGTGAATTAG

Upstream 100 bases:

>100_bases
CAATCGCACCTCAGCTTTGACGAATTCCTCGCGGTGGCGGCGAAACAGTCGACTCTGATGGTCGAGACGCTGGTACAGAA
ACTGGCGCATGGCTAAGCAA

Downstream 100 bases:

>100_bases
AGCCTGTCTTACCAGGCTCCCAGTGGGGATTCGACAATGCTCGTCTATTGGCTGGATATTGTAGGCACGGCTGTATTTGC
TATCTCTGGCGTGTTGCTGG

Product: vitamin B12-transporter protein BtuF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MFRALVALLLTLPVWLYAAPRVITLSPANTELAFAAGITPVGVSSYSDYPPEAQKIEQVSTWQGMNLERIVALKPDLVVA
WRGGNAERQVNQLTSLGIKVIWVDAVSIEQIADTLRQLAAWSPQPEKAQQAAQTLLNEYAALNAEYAGKAKKRVFLQFGM
NPLFTSGKGSIQHQVLTTCGGENVFADSRVPWPQVSREQVLARHPQAIIVAGKAGEILKIEQYWGNLLKIPVIPLNSDWF
ERASPRIILAAKQLCNALSQVN

Sequences:

>Translated_262_residues
MFRALVALLLTLPVWLYAAPRVITLSPANTELAFAAGITPVGVSSYSDYPPEAQKIEQVSTWQGMNLERIVALKPDLVVA
WRGGNAERQVNQLTSLGIKVIWVDAVSIEQIADTLRQLAAWSPQPEKAQQAAQTLLNEYAALNAEYAGKAKKRVFLQFGM
NPLFTSGKGSIQHQVLTTCGGENVFADSRVPWPQVSREQVLARHPQAIIVAGKAGEILKIEQYWGNLLKIPVIPLNSDWF
ERASPRIILAAKQLCNALSQVN
>Mature_262_residues
MFRALVALLLTLPVWLYAAPRVITLSPANTELAFAAGITPVGVSSYSDYPPEAQKIEQVSTWQGMNLERIVALKPDLVVA
WRGGNAERQVNQLTSLGIKVIWVDAVSIEQIADTLRQLAAWSPQPEKAQQAAQTLLNEYAALNAEYAGKAKKRVFLQFGM
NPLFTSGKGSIQHQVLTTCGGENVFADSRVPWPQVSREQVLARHPQAIIVAGKAGEILKIEQYWGNLLKIPVIPLNSDWF
ERASPRIILAAKQLCNALSQVN

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=262, Percent_Identity=81.2977099236641, Blast_Score=439, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 28794; Mature: 28794

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRALVALLLTLPVWLYAAPRVITLSPANTELAFAAGITPVGVSSYSDYPPEAQKIEQVS
CHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHH
TWQGMNLERIVALKPDLVVAWRGGNAERQVNQLTSLGIKVIWVDAVSIEQIADTLRQLAA
HCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHC
WSPQPEKAQQAAQTLLNEYAALNAEYAGKAKKRVFLQFGMNPLFTSGKGSIQHQVLTTCG
CCCCHHHHHHHHHHHHHHHHHCCCHHCCCHHHEEEEEECCCEEEECCCCCHHHHHHHHCC
GENVFADSRVPWPQVSREQVLARHPQAIIVAGKAGEILKIEQYWGNLLKIPVIPLNSDWF
CCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCEEEEHHHHCCEEEEEEEECCCHHH
ERASPRIILAAKQLCNALSQVN
HHCCCEEEEHHHHHHHHHHHCC
>Mature Secondary Structure
MFRALVALLLTLPVWLYAAPRVITLSPANTELAFAAGITPVGVSSYSDYPPEAQKIEQVS
CHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHH
TWQGMNLERIVALKPDLVVAWRGGNAERQVNQLTSLGIKVIWVDAVSIEQIADTLRQLAA
HCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHC
WSPQPEKAQQAAQTLLNEYAALNAEYAGKAKKRVFLQFGMNPLFTSGKGSIQHQVLTTCG
CCCCHHHHHHHHHHHHHHHHHCCCHHCCCHHHEEEEEECCCEEEECCCCCHHHHHHHHCC
GENVFADSRVPWPQVSREQVLARHPQAIIVAGKAGEILKIEQYWGNLLKIPVIPLNSDWF
CCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCEEEEHHHHCCEEEEEEEECCCHHH
ERASPRIILAAKQLCNALSQVN
HHCCCEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA