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The map label for this gene is bioH [H]

Identifier: 194737196

GI number: 194737196

Start: 3564899

End: 3565621

Strand: Reverse

Name: bioH [H]

Synonym: SeSA_A3708

Alternate gene names: 194737196

Gene position: 3565621-3564899 (Counterclockwise)

Preceding gene: 194734667

Following gene: 194736616

Centisome position: 75.72

GC content: 56.85

Gene sequence:

>723_bases
GTGCTGCTGCACGGATGGGGATTGAACGCGGAGGTATGGCATTGCATTCGCGAGGAACTCGGCTCGCATTTTACGCTGCA
TCTGGTCGATTTGCCGGGCTATGGCCGTAGTTCGGGGTTTGGCGCTATGACGTTTGAAGAGATGACGGCGCAGGTAGCGA
AGAACGCGCCGGACCAGGCTATCTGGCTTGGCTGGAGCCTGGGTGGCCTGGTGGCGAGTCAGATGGCGCTCACCCACCCT
GAACGCGTTCAGGCGCTGGTGACAGTCGCCTCTTCGCCATGCTTTAGCGCGCGTGAGGGGTGGCCGGGAATAAAACCGGA
AATCCTCGGTGGATTCCAGCAGCAGCTTAGCGACGATTTTCAGCGCACGGTGGAGCGTTTTCTGGCGCTGCAAACGTTAG
GGACGGAGACGGCGCGTCAGGATGCCCGCACCTTAAAAAGCGTGGTGCTGGCGCAGCCTATGCCGGATGTAGACGTGCTC
AATGGCGGACTGGAAATCTTAAAAACGGTTGATCTACGAGAAGCGCTTAAAAATGTGAACATGCCGTTTTTGCGTTTGTA
TGGTTATCTGGACGGTCTGGTGCCGCGTAAAATCGTGCCTTTGCTCGATACGTTATGGCCGCACAGTACATCACAGATAA
TGGCGAAGGCGGCCCATGCGCCGTTTATCTCGCATCCGGCGGCGTTTTGTCAGGCGCTGATGACGCTAAAATCATCGCTG
TAA

Upstream 100 bases:

>100_bases
CGGTACTGTTAGCATAGGTGCATCCTTGTTAGACAAAAGAGAACAGTAACTGATGAATGACATCTGGTGGCAGACCTACG
GCGAGGGAAATTGTCATCTT

Downstream 100 bases:

>100_bases
CCAGGGCGTTGCCGGGCCCGGACCATTCTGCCAGATTAATGGTTTTCAGATGCCAGATCGGCAGGCGCCAGCCCGGTAAC
CCTAAGCACGGTATCGCGAT

Product: carboxylesterase BioH

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MLLHGWGLNAEVWHCIREELGSHFTLHLVDLPGYGRSSGFGAMTFEEMTAQVAKNAPDQAIWLGWSLGGLVASQMALTHP
ERVQALVTVASSPCFSAREGWPGIKPEILGGFQQQLSDDFQRTVERFLALQTLGTETARQDARTLKSVVLAQPMPDVDVL
NGGLEILKTVDLREALKNVNMPFLRLYGYLDGLVPRKIVPLLDTLWPHSTSQIMAKAAHAPFISHPAAFCQALMTLKSSL

Sequences:

>Translated_240_residues
MLLHGWGLNAEVWHCIREELGSHFTLHLVDLPGYGRSSGFGAMTFEEMTAQVAKNAPDQAIWLGWSLGGLVASQMALTHP
ERVQALVTVASSPCFSAREGWPGIKPEILGGFQQQLSDDFQRTVERFLALQTLGTETARQDARTLKSVVLAQPMPDVDVL
NGGLEILKTVDLREALKNVNMPFLRLYGYLDGLVPRKIVPLLDTLWPHSTSQIMAKAAHAPFISHPAAFCQALMTLKSSL
>Mature_240_residues
MLLHGWGLNAEVWHCIREELGSHFTLHLVDLPGYGRSSGFGAMTFEEMTAQVAKNAPDQAIWLGWSLGGLVASQMALTHP
ERVQALVTVASSPCFSAREGWPGIKPEILGGFQQQLSDDFQRTVERFLALQTLGTETARQDARTLKSVVLAQPMPDVDVL
NGGLEILKTVDLREALKNVNMPFLRLYGYLDGLVPRKIVPLLDTLWPHSTSQIMAKAAHAPFISHPAAFCQALMTLKSSL

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=240, Percent_Identity=80, Blast_Score=406, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 26372; Mature: 26372

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLHGWGLNAEVWHCIREELGSHFTLHLVDLPGYGRSSGFGAMTFEEMTAQVAKNAPDQA
CEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCE
IWLGWSLGGLVASQMALTHPERVQALVTVASSPCFSAREGWPGIKPEILGGFQQQLSDDF
EEEEHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
QRTVERFLALQTLGTETARQDARTLKSVVLAQPMPDVDVLNGGLEILKTVDLREALKNVN
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
MPFLRLYGYLDGLVPRKIVPLLDTLWPHSTSQIMAKAAHAPFISHPAAFCQALMTLKSSL
CHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLLHGWGLNAEVWHCIREELGSHFTLHLVDLPGYGRSSGFGAMTFEEMTAQVAKNAPDQA
CEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCE
IWLGWSLGGLVASQMALTHPERVQALVTVASSPCFSAREGWPGIKPEILGGFQQQLSDDF
EEEEHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
QRTVERFLALQTLGTETARQDARTLKSVVLAQPMPDVDVLNGGLEILKTVDLREALKNVN
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
MPFLRLYGYLDGLVPRKIVPLLDTLWPHSTSQIMAKAAHAPFISHPAAFCQALMTLKSSL
CHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA