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The map label for this gene is nrfF [H]

Identifier: 194737153

GI number: 194737153

Start: 4412116

End: 4412625

Strand: Direct

Name: nrfF [H]

Synonym: SeSA_A4534

Alternate gene names: 194737153

Gene position: 4412116-4412625 (Clockwise)

Preceding gene: 194735440

Following gene: 194738127

Centisome position: 93.69

GC content: 55.1

Gene sequence:

>510_bases
ATGCGTAAATTACTCACAGCGTTGCCGGATGGCGGCGTAAACGCCTTATCCGGCCTACAGGATGCGCAAACCGTAGGTCG
GATAAGCGTAGCGCCATCCGGCAGAAAGTTTTTACTCTTAACGATCGTCCTGTTTTTAATCACCTTCGCCACCCACGCCC
AGGTGGTGGATACCTGGCGCTTTGACAATCCGCAACAGCAGGAAAAAGCGCTGTCTATTGCCAGTCAACTGCGTTGTCCG
CAGTGTCAGAACCAGAATTTGCTGGAATCCAATGCGCCCGTGGCGGTCAGTATGCGCCATCAGGTTTACAGTATGGTGGC
CGAAGGTAAAAGCGAAGCGGAAATCACCGCCTGGATGACCGACCGTTATGGTGATTTCGTACGCTACAACCCGCCGTTGA
ATGAGCAAACGCTGCTGTTGTGGGCGCTGCCGTGCCTGCTGTTATTGCTGGTGGGCGTGGTGGTCTGGCGGGCGAGAAAG
CGTCAGCACGCAGAAGAGGGGGAACAATGA

Upstream 100 bases:

>100_bases
ATGCGCTTATACGTGCAGACCGGCGTGCGCTGGATCTGGGGTGGGGGATTGTTGATGGTGTGTGGCGCGCTGCTGAGCGG
ATGGCGGGGGAGGAAGCGTG

Downstream 100 bases:

>100_bases
GCCAGTCTGAACACTCCACTGCGCCGTTACGTCCCATGCCCGTAAAGCGTCTGGCGGCTGCGGCGGTGTTGATGGTGGCT
GCCTGTATCGGCGGCTATAT

Product: formate-dependent nitrite reductase complex subunit NrfF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MRKLLTALPDGGVNALSGLQDAQTVGRISVAPSGRKFLLLTIVLFLITFATHAQVVDTWRFDNPQQQEKALSIASQLRCP
QCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEAEITAWMTDRYGDFVRYNPPLNEQTLLLWALPCLLLLLVGVVVWRARK
RQHAEEGEQ

Sequences:

>Translated_169_residues
MRKLLTALPDGGVNALSGLQDAQTVGRISVAPSGRKFLLLTIVLFLITFATHAQVVDTWRFDNPQQQEKALSIASQLRCP
QCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEAEITAWMTDRYGDFVRYNPPLNEQTLLLWALPCLLLLLVGVVVWRARK
RQHAEEGEQ
>Mature_169_residues
MRKLLTALPDGGVNALSGLQDAQTVGRISVAPSGRKFLLLTIVLFLITFATHAQVVDTWRFDNPQQQEKALSIASQLRCP
QCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEAEITAWMTDRYGDFVRYNPPLNEQTLLLWALPCLLLLLVGVVVWRARK
RQHAEEGEQ

Specific function: Not required for the biosynthesis of any of the c-type cytochromes. Possible subunit of a heme lyase [H]

COG id: COG3088

COG function: function code O; Uncharacterized protein involved in biosynthesis of c-type cytochromes

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CcmH/CycL/Ccl2/NrfF family [H]

Homologues:

Organism=Escherichia coli, GI1790512, Length=91, Percent_Identity=82.4175824175824, Blast_Score=154, Evalue=2e-39,
Organism=Escherichia coli, GI1788522, Length=119, Percent_Identity=36.9747899159664, Blast_Score=102, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005616
- InterPro:   IPR017565 [H]

Pfam domain/function: PF03918 CcmH [H]

EC number: NA

Molecular weight: Translated: 18928; Mature: 18928

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLLTALPDGGVNALSGLQDAQTVGRISVAPSGRKFLLLTIVLFLITFATHAQVVDTWR
CCCHHHHCCCCCHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
FDNPQQQEKALSIASQLRCPQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEAEITAWMT
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHH
DRYGDFVRYNPPLNEQTLLLWALPCLLLLLVGVVVWRARKRQHAEEGEQ
HCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRKLLTALPDGGVNALSGLQDAQTVGRISVAPSGRKFLLLTIVLFLITFATHAQVVDTWR
CCCHHHHCCCCCHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
FDNPQQQEKALSIASQLRCPQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEAEITAWMT
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHH
DRYGDFVRYNPPLNEQTLLLWALPCLLLLLVGVVVWRARKRQHAEEGEQ
HCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]