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The map label for this gene is priA [H]

Identifier: 194737086

GI number: 194737086

Start: 4180442

End: 4182640

Strand: Reverse

Name: priA [H]

Synonym: SeSA_A4311

Alternate gene names: 194737086

Gene position: 4182640-4180442 (Counterclockwise)

Preceding gene: 194735881

Following gene: 194736341

Centisome position: 88.82

GC content: 59.57

Gene sequence:

>2199_bases
ATGTCCGTCGCGCACGTTGCCTTGCCCGTTCCGCTTCCCCGAACCTTTGATTACCTGCTGCCGGAAGGCGAGGTCGCCAA
AGCCGGGTGCCGCGTGCGCGTTCCGTTCGGCAAGCAGCAAGAGCGCGTGGGCATTGTCGTCTCGGTTAGCGACCACAGCG
AATTGCCGCTCGATGAATTAAAATCGGTCATTGAAATCCTGGATAACGAGCCCATCTTTTCTCCCTCCATCTGGCGCTTA
CTCCTCTGGGCGGCGGATTACTACCACCATCCCCTCGGCGACGTTCTGTTTCACGCTCTGCCGGTTTTACTGCGTCAGGG
AAAACCAGCCAGCAACGCGCCCCTATGGTATTGGTTCGCCACCGAAGAGGGGCAAGCCGCCGACATCAACAGCCTGAAAC
GCTCCGCCAAACAACAACAGGCGCTGGCGGCGCTACGGCAAGGAAAGATCTGGCGCTATCAGGTAGCCGAGCTTGATTTT
ACTGACGCCACGCTGCAAACGCTGCGGCGTAAGGGGCTTTGTGAGCTTGCCAGTGAAACGCCCGCGTTTACCGACTGGCG
GGAGCATTATGCCGTCGCAGGAGAGCGCCTGCGGCTAAATACCGAACAGGCGACAGCGGTCGGCGCGATTCACAGCGCGT
CGGACGGTTTCTCCGCCTGGTTGCTGGCAGGCGTAACCGGTTCCGGCAAGACCGAAGTGTATCTGAGCGTGCTGGAAAAC
GTCCTCGCCCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACGCCGCAAACGATCGCCCGTTTTCGCGA
ACGTTTTAACGCGCCGGTGGAAGTGCTGCATTCCGGGCTAAACGACAGCGAGCGTCTTTCGGCATGGCTGAAAGCGAAAA
ACGGCGAAGCGGCGATTGTCATCGGCACGCGCTCCTCGCTGTTTACGCCGTTTAAAAATCTTGGCGTGATTGTCATCGAC
GAAGAGCACGACAGCTCTTATAAACAGCAGGAAGGGTGGCGCTACCATGCCCGCGATTTGGCTGTGTATCGCGCCCATAG
CGAACAGATCCCGATTATTCTCGGCTCCGCCACCCCAGCCCTGGAAACGCTATGCAACGTGCGGCAGAAAAAATACCGGA
TGCTGCGTCTGACGCGGCGCGCAGGCAACGCCCGCCCAGCCCTGCAGCATGTACTGGATTTAAAAGGCCAGCGTCTTCAG
GCGGGACTGGCGCCCGCCCTTATCGCCCGTATGCGCCAACATCTCCAGGCCGACAATCAGGTAATTTTATTTCTTAACCG
CCGGGGATTCGCGCCCGCGCTGCTTTGCCACGACTGCGGCTGGATTGCGGAGTGCCCGCGTTGCGATCACTACTACACGC
TGCACCAGGCGCAGCATCATTTACGCTGTCATCACTGCGACAGCCAGCGTCCGGTACCGCGTCAGTGCCCCTCCTGCGGC
TCCACACATATGCTACCGGTTGGGTTAGGGACGGAACAGCTGGAGCAGGTGCTGGCGCCCTTCTTCCCCGGCGTGCCGAT
CTCACGAATCGACCGTGATACCACCAGTCGTAAAGGCGCGCTGGAGCAGCATCTGGCGGAAGTTCACCGCGGCGGAGCGC
GCATTCTGATAGGCACACAAATGCTGGCGAAAGGCCATCACTTCCCGGACGTCACGCTGGTGGCGTTGCTGGACGTCGAT
GGCGCGCTTTTTTCCGCAGATTTTCGTTCGGCGGAACGCTTCGCCCAGCTCTACACTCAGGTTTCCGGCCGCGCCGGGCG
CGCGGGTAAACAGGGCGAAGTGGTGCTCCAAACGCACCATCCTGAGCATCCCCTGCTGCAAACGTTACTGTATAAAGGCT
ATGATGCGTTTGCGGAGCAGGCGCTGGCGGAACGGCAAACGCTCCAGCTTCCGCCGTGGACCAGCCACGTCATTATTCGC
GCAGAGGATCACAACAATCAGCAGGCGCCCGTCTTTCTCCAGCAGTTGCGTAATCTGATTCAGGCCAGCCCGCTATCAGA
CGATAAGCTGTGGATCTTAGGTCCGGTTCCGGCCCTCGCGCCAAAACGCGGCGGCCGCTATCGCTGGCAAATTTTACTTC
AGCATCCTTCACGGATTCGCCTGCAACACATCATCAGCGGCACGCTGGCGTTGATCAATACGCTCCCTGAAGCGCGGAAA
GTTAAATGGGTTCTGGACGTCGATCCGATTGAAGGATAG

Upstream 100 bases:

>100_bases
GGCGAATCATACAGAATTTGACCACCGTATGCAAACTGATCCGCGCTCCCTTATCGTAATGTGTATACTACCCGACGAAA
TTTCAAGTCAGGAAGATGCT

Downstream 100 bases:

>100_bases
TCCTCACGATGCGAGGCGGATCGTAAAATTAAAGATCCATCACACTTTTTATGAAAATTCTGTAACCGCTTCCACGCACT
ATCTGATAAAAATGTTTTCG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 732; Mature: 731

Protein sequence:

>732_residues
MSVAHVALPVPLPRTFDYLLPEGEVAKAGCRVRVPFGKQQERVGIVVSVSDHSELPLDELKSVIEILDNEPIFSPSIWRL
LLWAADYYHHPLGDVLFHALPVLLRQGKPASNAPLWYWFATEEGQAADINSLKRSAKQQQALAALRQGKIWRYQVAELDF
TDATLQTLRRKGLCELASETPAFTDWREHYAVAGERLRLNTEQATAVGAIHSASDGFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSSLFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVRQKKYRMLRLTRRAGNARPALQHVLDLKGQRLQ
AGLAPALIARMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQHHLRCHHCDSQRPVPRQCPSCG
STHMLPVGLGTEQLEQVLAPFFPGVPISRIDRDTTSRKGALEQHLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERQTLQLPPWTSHVIIR
AEDHNNQQAPVFLQQLRNLIQASPLSDDKLWILGPVPALAPKRGGRYRWQILLQHPSRIRLQHIISGTLALINTLPEARK
VKWVLDVDPIEG

Sequences:

>Translated_732_residues
MSVAHVALPVPLPRTFDYLLPEGEVAKAGCRVRVPFGKQQERVGIVVSVSDHSELPLDELKSVIEILDNEPIFSPSIWRL
LLWAADYYHHPLGDVLFHALPVLLRQGKPASNAPLWYWFATEEGQAADINSLKRSAKQQQALAALRQGKIWRYQVAELDF
TDATLQTLRRKGLCELASETPAFTDWREHYAVAGERLRLNTEQATAVGAIHSASDGFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSSLFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVRQKKYRMLRLTRRAGNARPALQHVLDLKGQRLQ
AGLAPALIARMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQHHLRCHHCDSQRPVPRQCPSCG
STHMLPVGLGTEQLEQVLAPFFPGVPISRIDRDTTSRKGALEQHLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERQTLQLPPWTSHVIIR
AEDHNNQQAPVFLQQLRNLIQASPLSDDKLWILGPVPALAPKRGGRYRWQILLQHPSRIRLQHIISGTLALINTLPEARK
VKWVLDVDPIEG
>Mature_731_residues
SVAHVALPVPLPRTFDYLLPEGEVAKAGCRVRVPFGKQQERVGIVVSVSDHSELPLDELKSVIEILDNEPIFSPSIWRLL
LWAADYYHHPLGDVLFHALPVLLRQGKPASNAPLWYWFATEEGQAADINSLKRSAKQQQALAALRQGKIWRYQVAELDFT
DATLQTLRRKGLCELASETPAFTDWREHYAVAGERLRLNTEQATAVGAIHSASDGFSAWLLAGVTGSGKTEVYLSVLENV
LAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSSLFTPFKNLGVIVIDE
EHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVRQKKYRMLRLTRRAGNARPALQHVLDLKGQRLQA
GLAPALIARMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQHHLRCHHCDSQRPVPRQCPSCGS
THMLPVGLGTEQLEQVLAPFFPGVPISRIDRDTTSRKGALEQHLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDG
ALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERQTLQLPPWTSHVIIRA
EDHNNQQAPVFLQQLRNLIQASPLSDDKLWILGPVPALAPKRGGRYRWQILLQHPSRIRLQHIISGTLALINTLPEARKV
KWVLDVDPIEG

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=732, Percent_Identity=90.5737704918033, Blast_Score=1395, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81784; Mature: 81653

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAHVALPVPLPRTFDYLLPEGEVAKAGCRVRVPFGKQQERVGIVVSVSDHSELPLDEL
CCCEEEEECCCCCCCHHHHCCCCCEECCCCEEECCCCCCCCCEEEEEEECCCCCCCHHHH
KSVIEILDNEPIFSPSIWRLLLWAADYYHHPLGDVLFHALPVLLRQGKPASNAPLWYWFA
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
TEEGQAADINSLKRSAKQQQALAALRQGKIWRYQVAELDFTDATLQTLRRKGLCELASET
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHCCHHHHHHCC
PAFTDWREHYAVAGERLRLNTEQATAVGAIHSASDGFSAWLLAGVTGSGKTEVYLSVLEN
CCCHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHH
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIV
HHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHCCCCCCEEEE
IGTRSSLFTPFKNLGVIVIDEEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPA
EECCHHHHCHHHHCCEEEEECCCCCCHHHHCCCEEHHHHEEEEEECCCCCCEEEECCCHH
LETLCNVRQKKYRMLRLTRRAGNARPALQHVLDLKGQRLQAGLAPALIARMRQHLQADNQ
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHCCCCC
VILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQHHLRCHHCDSQRPVPRQCPSCG
EEEEEECCCCCCHHHHHCCCCHHCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCC
STHMLPVGLGTEQLEQVLAPFFPGVPISRIDRDTTSRKGALEQHLAEVHRGGARILIGTQ
CCCEEEECCCHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHH
MLAKGHHFPDVTLVALLDVDGALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVVLQTHH
HHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECC
PEHPLLQTLLYKGYDAFAEQALAERQTLQLPPWTSHVIIRAEDHNNQQAPVFLQQLRNLI
CCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHH
QASPLSDDKLWILGPVPALAPKRGGRYRWQILLQHPSRIRLQHIISGTLALINTLPEARK
HCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCE
VKWVLDVDPIEG
EEEEEECCCCCC
>Mature Secondary Structure 
SVAHVALPVPLPRTFDYLLPEGEVAKAGCRVRVPFGKQQERVGIVVSVSDHSELPLDEL
CCEEEEECCCCCCCHHHHCCCCCEECCCCEEECCCCCCCCCEEEEEEECCCCCCCHHHH
KSVIEILDNEPIFSPSIWRLLLWAADYYHHPLGDVLFHALPVLLRQGKPASNAPLWYWFA
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
TEEGQAADINSLKRSAKQQQALAALRQGKIWRYQVAELDFTDATLQTLRRKGLCELASET
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHCCHHHHHHCC
PAFTDWREHYAVAGERLRLNTEQATAVGAIHSASDGFSAWLLAGVTGSGKTEVYLSVLEN
CCCHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHH
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIV
HHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHCCCCCCEEEE
IGTRSSLFTPFKNLGVIVIDEEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPA
EECCHHHHCHHHHCCEEEEECCCCCCHHHHCCCEEHHHHEEEEEECCCCCCEEEECCCHH
LETLCNVRQKKYRMLRLTRRAGNARPALQHVLDLKGQRLQAGLAPALIARMRQHLQADNQ
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHCCCCC
VILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQHHLRCHHCDSQRPVPRQCPSCG
EEEEEECCCCCCHHHHHCCCCHHCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCC
STHMLPVGLGTEQLEQVLAPFFPGVPISRIDRDTTSRKGALEQHLAEVHRGGARILIGTQ
CCCEEEECCCHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHH
MLAKGHHFPDVTLVALLDVDGALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVVLQTHH
HHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECC
PEHPLLQTLLYKGYDAFAEQALAERQTLQLPPWTSHVIIRAEDHNNQQAPVFLQQLRNLI
CCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHH
QASPLSDDKLWILGPVPALAPKRGGRYRWQILLQHPSRIRLQHIISGTLALINTLPEARK
HCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCE
VKWVLDVDPIEG
EEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]