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The map label for this gene is cusS [H]

Identifier: 194736868

GI number: 194736868

Start: 701513

End: 701887

Strand: Reverse

Name: cusS [H]

Synonym: SeSA_A0727

Alternate gene names: 194736868

Gene position: 701887-701513 (Counterclockwise)

Preceding gene: 194734422

Following gene: 194736386

Centisome position: 14.9

GC content: 51.2

Gene sequence:

>375_bases
TTGCGCTTTGAAGGCCGCGCCTGCTGGATCACCGGCGATCCCATCATGCTGCGCAGAGCGATCAGTAACTTACTCTCTAA
TGCGATGCACTATACGCCGCCGGGTAAAACCATTACTCTTCGGATAAAAGAAGCGGACGACCAGATACATATTATTGTTG
AAAACCCCGGTACGCCAATCGCGCCGGAACATTTGCCGCGCCTTTTCGATCGTTTTTATCGGGTTGATCCCTCCCGCCAG
CGTAAAGGGGAAGGCAGCGGTATAGGCCTGGCAATTGTAAAATCCATCGTCATTGCGCATCAGGGAAAAGTTTCTGTCAC
CTCCGATACGCGCGCTACTCGTTTTATTTTGACATTACCCAAACACCTCCGTTGA

Upstream 100 bases:

>100_bases
CGATAATCACCGCTACCGTCATGGCTTTTGGCCGCACCCGGAGCACCGCCCCGTGGTAAAGCGTCTCATCGAGTTTTTCT
GCCGAAAAGGTGTTGGGAGC

Downstream 100 bases:

>100_bases
ACTGTAGCGATAGTGAAAGGCGCTATCACTAGCCAGCAAAATAATCTGACATCATCGTCATTATCCCGTCACGCGCCAGA
CCCAACGACTGTTTTATTAT

Product: sensor kinase CusS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 124; Mature: 124

Protein sequence:

>124_residues
MRFEGRACWITGDPIMLRRAISNLLSNAMHYTPPGKTITLRIKEADDQIHIIVENPGTPIAPEHLPRLFDRFYRVDPSRQ
RKGEGSGIGLAIVKSIVIAHQGKVSVTSDTRATRFILTLPKHLR

Sequences:

>Translated_124_residues
MRFEGRACWITGDPIMLRRAISNLLSNAMHYTPPGKTITLRIKEADDQIHIIVENPGTPIAPEHLPRLFDRFYRVDPSRQ
RKGEGSGIGLAIVKSIVIAHQGKVSVTSDTRATRFILTLPKHLR
>Mature_124_residues
MRFEGRACWITGDPIMLRRAISNLLSNAMHYTPPGKTITLRIKEADDQIHIIVENPGTPIAPEHLPRLFDRFYRVDPSRQ
RKGEGSGIGLAIVKSIVIAHQGKVSVTSDTRATRFILTLPKHLR

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=120, Percent_Identity=73.3333333333333, Blast_Score=194, Evalue=2e-51,
Organism=Escherichia coli, GI1786600, Length=115, Percent_Identity=45.2173913043478, Blast_Score=100, Evalue=3e-23,
Organism=Escherichia coli, GI145693157, Length=114, Percent_Identity=35.9649122807018, Blast_Score=76, Evalue=7e-16,
Organism=Escherichia coli, GI1787894, Length=109, Percent_Identity=38.5321100917431, Blast_Score=72, Evalue=9e-15,
Organism=Escherichia coli, GI1788393, Length=95, Percent_Identity=34.7368421052632, Blast_Score=72, Evalue=1e-14,
Organism=Escherichia coli, GI1790346, Length=116, Percent_Identity=31.8965517241379, Blast_Score=66, Evalue=6e-13,
Organism=Escherichia coli, GI1788279, Length=114, Percent_Identity=35.9649122807018, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI87081816, Length=114, Percent_Identity=36.8421052631579, Blast_Score=62, Evalue=8e-12,
Organism=Escherichia coli, GI87082128, Length=107, Percent_Identity=33.6448598130841, Blast_Score=61, Evalue=2e-11,
Organism=Escherichia coli, GI48994928, Length=113, Percent_Identity=32.7433628318584, Blast_Score=60, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 13951; Mature: 13951

Theoretical pI: Translated: 11.03; Mature: 11.03

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFEGRACWITGDPIMLRRAISNLLSNAMHYTPPGKTITLRIKEADDQIHIIVENPGTPI
CCCCCCEEEEECCHHHHHHHHHHHHHCCEECCCCCCEEEEEEEECCCEEEEEEECCCCCC
APEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVIAHQGKVSVTSDTRATRFILTLP
CHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCEEEEEECC
KHLR
CCCC
>Mature Secondary Structure
MRFEGRACWITGDPIMLRRAISNLLSNAMHYTPPGKTITLRIKEADDQIHIIVENPGTPI
CCCCCCEEEEECCHHHHHHHHHHHHHCCEECCCCCCEEEEEEEECCCEEEEEEECCCCCC
APEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVIAHQGKVSVTSDTRATRFILTLP
CHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCEEEEEECC
KHLR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]