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The map label for this gene is yjjW [H]

Identifier: 194736787

GI number: 194736787

Start: 4675198

End: 4676061

Strand: Reverse

Name: yjjW [H]

Synonym: SeSA_A4819

Alternate gene names: 194736787

Gene position: 4676061-4675198 (Counterclockwise)

Preceding gene: 194735927

Following gene: 194736271

Centisome position: 99.3

GC content: 55.67

Gene sequence:

>864_bases
ATGAACAACAGATGCGCTTTAGTCAGTAAGATCATTGCGTTTTCCTGCGTCGATGGGCCAGGCAGCCGCCTGGCATTATT
CTTACAGGGCTGCAATTTGCGCTGTAAGAATTGCCATAACCCGTGGACGATGGGACGCTGTAACGACTGCGGGGAATGCG
TATCGCAGTGTCCGCATCACGCCCTCAGCGTTGATGGCGGGAAAGTGGTATGGCGGTCGGACGTCTGCGAGCAGTGCGAT
ACCTGCCTGAAAATGTGCCCACAGCGGGCAACGCCAATGGCGCAAACCATGAGCGTTGACGATGTGCTGCGTCATATTCG
TAAAGCATCGCTGTTTATTGAAGGTATTACCGTCAGCGGCGGAGAGGCGACCACGCAATTACCGTTTATTGTGGCGCTAT
TTACGGCGATCAAAGCCGATCCGCAGTTACAACGGCTGACCTGTCTGGTGGACAGTAATGGTCAGTTAAGCGAGACCGGC
TGGCAAAAACTACTGCCGGTTTGCGACGGCGTAATGCTCGATCTGAAAGCCTGGAAGAGCGAGTGTCATCACCGTCTGAC
CGGACGTGATAACACGCATATTAAGCGCAGTATCCGCTTTCTTGCTGCGCGGGGAAAACTGGCGGAGCTGCGGTTGCTGG
TTATCCCGGGTCAGGTGGACTATGCGGCGCATATCGATTCACTGGCGGCGTTTATTACTTCGCTGGGCGAGGTTCCGGTG
CGTTTGAATGCCTTTCATGCGCATGGCGTCTATGGCGAAGCGAAAGCCTGGCCAGGCGCGACGCCGCAAGAGGTGGAACA
GCTGGCTGACGGATTGCGCGCGCGTGGGGTGGCGAAACTGATTTTGCCGGCGCTATATTTGTAA

Upstream 100 bases:

>100_bases
AAATTTCGCGCCGAAGGCTCGCGCACGAATACCACCTGGCTGGGAGAAGAAGCCGCACGTAATACCCGCATCCTGGAACG
ACAGCCACGCGTAGTCAGCC

Downstream 100 bases:

>100_bases
GCGTCACCTGCCGGACAGCGGCTGCGATGCCCTCAGAGCCGAAACAAGGTAATGGTATTGCGATACAGCGTATCGGCTAT
CACCTCTGCGGGCTCCGGAC

Product: pyruvate formate lyase activating enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MNNRCALVSKIIAFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVSQCPHHALSVDGGKVVWRSDVCEQCD
TCLKMCPQRATPMAQTMSVDDVLRHIRKASLFIEGITVSGGEATTQLPFIVALFTAIKADPQLQRLTCLVDSNGQLSETG
WQKLLPVCDGVMLDLKAWKSECHHRLTGRDNTHIKRSIRFLAARGKLAELRLLVIPGQVDYAAHIDSLAAFITSLGEVPV
RLNAFHAHGVYGEAKAWPGATPQEVEQLADGLRARGVAKLILPALYL

Sequences:

>Translated_287_residues
MNNRCALVSKIIAFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVSQCPHHALSVDGGKVVWRSDVCEQCD
TCLKMCPQRATPMAQTMSVDDVLRHIRKASLFIEGITVSGGEATTQLPFIVALFTAIKADPQLQRLTCLVDSNGQLSETG
WQKLLPVCDGVMLDLKAWKSECHHRLTGRDNTHIKRSIRFLAARGKLAELRLLVIPGQVDYAAHIDSLAAFITSLGEVPV
RLNAFHAHGVYGEAKAWPGATPQEVEQLADGLRARGVAKLILPALYL
>Mature_287_residues
MNNRCALVSKIIAFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVSQCPHHALSVDGGKVVWRSDVCEQCD
TCLKMCPQRATPMAQTMSVDDVLRHIRKASLFIEGITVSGGEATTQLPFIVALFTAIKADPQLQRLTCLVDSNGQLSETG
WQKLLPVCDGVMLDLKAWKSECHHRLTGRDNTHIKRSIRFLAARGKLAELRLLVIPGQVDYAAHIDSLAAFITSLGEVPV
RLNAFHAHGVYGEAKAWPGATPQEVEQLADGLRARGVAKLILPALYL

Specific function: Unknown

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790839, Length=287, Percent_Identity=81.5331010452962, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI1787130, Length=283, Percent_Identity=29.6819787985866, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790389, Length=260, Percent_Identity=24.6153846153846, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 31372; Mature: 31372

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS01087 RADICAL_ACTIVATING ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
5.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNRCALVSKIIAFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVSQCPHH
CCCHHHHHHHHHHHHCCCCCCHHEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHCCCC
ALSVDGGKVVWRSDVCEQCDTCLKMCPQRATPMAQTMSVDDVLRHIRKASLFIEGITVSG
EEECCCCEEEEHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHEEEEEEECC
GEATTQLPFIVALFTAIKADPQLQRLTCLVDSNGQLSETGWQKLLPVCDGVMLDLKAWKS
CCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHH
ECHHRLTGRDNTHIKRSIRFLAARGKLAELRLLVIPGQVDYAAHIDSLAAFITSLGEVPV
HHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCE
RLNAFHAHGVYGEAKAWPGATPQEVEQLADGLRARGVAKLILPALYL
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MNNRCALVSKIIAFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVSQCPHH
CCCHHHHHHHHHHHHCCCCCCHHEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHCCCC
ALSVDGGKVVWRSDVCEQCDTCLKMCPQRATPMAQTMSVDDVLRHIRKASLFIEGITVSG
EEECCCCEEEEHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHEEEEEEECC
GEATTQLPFIVALFTAIKADPQLQRLTCLVDSNGQLSETGWQKLLPVCDGVMLDLKAWKS
CCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHH
ECHHRLTGRDNTHIKRSIRFLAARGKLAELRLLVIPGQVDYAAHIDSLAAFITSLGEVPV
HHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCE
RLNAFHAHGVYGEAKAWPGATPQEVEQLADGLRARGVAKLILPALYL
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]