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The map label for this gene is wcaD [H]

Identifier: 194736759

GI number: 194736759

Start: 2216621

End: 2217835

Strand: Reverse

Name: wcaD [H]

Synonym: SeSA_A2345

Alternate gene names: 194736759

Gene position: 2217835-2216621 (Counterclockwise)

Preceding gene: 194736263

Following gene: 194734994

Centisome position: 47.1

GC content: 43.13

Gene sequence:

>1215_bases
ATGTCTCGTTCTATCAGAATCTGTAGCTACCTGCTGCTGCCGCTGATCTACCTACTGGTCAATGTCAAGATTGCCCAACT
GGGGGAAAGCTTTCCCATTACCATCGTCACTTTTTTACCGCTGTTGCTGCTGTTATTTGTGGAACGCATTAGCGTAAAAA
AATTGATGATCGCCTTAGGCATCGGCGCGGGGCTTACGGCATTTAACTTCCTGTTCGGCCAGTCGCTGAATGCTGGTAAA
TATGTCACGTCCACGATGCTGTTTGTCTATATTGTGGTCATTATCGGGATGGTCTGGAGTATCCGATTCAAAACCATTTC
CGCGCATAACCACCGAAAGATTTTGCGTTTTTTTTATCTGGTGGTGGGTATAGTGGTCGCGCTCGCCGCGGTTGAGATGG
CGCAAATTATCCTTACCGGCGGCAGCAGTATTATGGAAGGAATTTCGAAATATCTCATTTACAGTAATAGCTACGTACTG
AACTTCATAAAATTTGGCGGTAAGCGTACCACTGCGCTTTATTTTGAACCGGCATTTTTCGCTTTGGCACTAATCTCAAT
TTGGCTGAGCATCAAACAGTTTGGTATCAAAACACCGAAAAGCGATGCTATGATTCTGGCAGGGATAATATTATCAGGAT
CATTTTCAGGGGTAATGACCTTTATCCTGTTTTATCTTCTGGAGTGGGCGTTCCAATATTTGAATAAGGATGCGATAAAG
AAAAAACTTCCACTGGCGCTGGTCTCATTAACCCTGTTTTTGGTTGGGGTAATTATTGCATTTCCTTATATCGCGACACG
ACTTGGCGATTTAGGGACGGAAGGCTCATCTTCTTATTATCGTATTGTGGGACCCTTAGTCATGGTCGGATATTCCTTGA
CCCATATTGATGGTGTAGTCAGATTTGGCTCACTTTATGAATATGTCGCATCATTCGGAATCTTTAACGGTGCGGATGTC
GGGAAAACCATAGACAATGGATTGTATCTGCTGATTATTTATTTTTCCTGGTTCGCAGTGCTAATGACGCTGTGGTATAT
GGGGAAAGTTTTAAAAATGGCGCTAAATGCGTTTGGCGATAATCGCAATTTTCGGGTGCAGCTCTATCTTTTTACGCCGG
TGTCGCTGTTTTTTACCGGTTCAATATTTAGCCCGGAATATGCTTTTTTAATCGTCTGTCCGTTCATTTTGCGCAAGGCG
TTAAAAATTTCATAA

Upstream 100 bases:

>100_bases
CGTAAGCCAGAGATTGCTCAGGCGGTATTTGGCGCAACGCTGGACGCCTTTCGTATGCGTAGCCGCGTCGCGTACAGCGG
TCAACAGATGCTGGAGGAGT

Downstream 100 bases:

>100_bases
CGGGGTGGTGGCATGTTTTTAAGCGTCGTTACCGTCGCCTTTCGCAATTACGAAGGGGTGGTAAAAACCTGGCGCTCGCT
GCGCAATCTGGCGCGCGATC

Product: putative colanic acid biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSRSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPLLLLLFVERISVKKLMIALGIGAGLTAFNFLFGQSLNAGK
YVTSTMLFVYIVVIIGMVWSIRFKTISAHNHRKILRFFYLVVGIVVALAAVEMAQIILTGGSSIMEGISKYLIYSNSYVL
NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKSDAMILAGIILSGSFSGVMTFILFYLLEWAFQYLNKDAIK
KKLPLALVSLTLFLVGVIIAFPYIATRLGDLGTEGSSSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADV
GKTIDNGLYLLIIYFSWFAVLMTLWYMGKVLKMALNAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIVCPFILRKA
LKIS

Sequences:

>Translated_404_residues
MSRSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPLLLLLFVERISVKKLMIALGIGAGLTAFNFLFGQSLNAGK
YVTSTMLFVYIVVIIGMVWSIRFKTISAHNHRKILRFFYLVVGIVVALAAVEMAQIILTGGSSIMEGISKYLIYSNSYVL
NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKSDAMILAGIILSGSFSGVMTFILFYLLEWAFQYLNKDAIK
KKLPLALVSLTLFLVGVIIAFPYIATRLGDLGTEGSSSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADV
GKTIDNGLYLLIIYFSWFAVLMTLWYMGKVLKMALNAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIVCPFILRKA
LKIS
>Mature_403_residues
SRSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPLLLLLFVERISVKKLMIALGIGAGLTAFNFLFGQSLNAGKY
VTSTMLFVYIVVIIGMVWSIRFKTISAHNHRKILRFFYLVVGIVVALAAVEMAQIILTGGSSIMEGISKYLIYSNSYVLN
FIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKSDAMILAGIILSGSFSGVMTFILFYLLEWAFQYLNKDAIKK
KLPLALVSLTLFLVGVIIAFPYIATRLGDLGTEGSSSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVG
KTIDNGLYLLIIYFSWFAVLMTLWYMGKVLKMALNAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIVCPFILRKAL
KIS

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788369, Length=404, Percent_Identity=93.8118811881188, Blast_Score=720, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 45130; Mature: 44999

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPLLLLLFVERISVKKLMIALG
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
IGAGLTAFNFLFGQSLNAGKYVTSTMLFVYIVVIIGMVWSIRFKTISAHNHRKILRFFYL
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHH
VVGIVVALAAVEMAQIILTGGSSIMEGISKYLIYSNSYVLNFIKFGGKRTTALYFEPAFF
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEECHHHH
ALALISIWLSIKQFGIKTPKSDAMILAGIILSGSFSGVMTFILFYLLEWAFQYLNKDAIK
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHH
KKLPLALVSLTLFLVGVIIAFPYIATRLGDLGTEGSSSYYRIVGPLVMVGYSLTHIDGVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIYFSWFAVLMTLWYMGKVLKMALNAFGD
HHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIVCPFILRKALKIS
CCCEEEEEEEECCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPLLLLLFVERISVKKLMIALG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
IGAGLTAFNFLFGQSLNAGKYVTSTMLFVYIVVIIGMVWSIRFKTISAHNHRKILRFFYL
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHH
VVGIVVALAAVEMAQIILTGGSSIMEGISKYLIYSNSYVLNFIKFGGKRTTALYFEPAFF
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEECHHHH
ALALISIWLSIKQFGIKTPKSDAMILAGIILSGSFSGVMTFILFYLLEWAFQYLNKDAIK
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHH
KKLPLALVSLTLFLVGVIIAFPYIATRLGDLGTEGSSSYYRIVGPLVMVGYSLTHIDGVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIYFSWFAVLMTLWYMGKVLKMALNAFGD
HHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIVCPFILRKALKIS
CCCEEEEEEEECCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9278503 [H]