Click here to switch to the map view.

The map label for this gene is nuoB [H]

Identifier: 194736744

GI number: 194736744

Start: 2441476

End: 2442138

Strand: Reverse

Name: nuoB [H]

Synonym: SeSA_A2555

Alternate gene names: 194736744

Gene position: 2442138-2441476 (Counterclockwise)

Preceding gene: 194736597

Following gene: 194736818

Centisome position: 51.86

GC content: 54.15

Gene sequence:

>663_bases
ATGGATTATACGCTCACCCGCATAGATCCTAACGGTGAGAATGACCGTTACCCCCTGCAAAAACAGGAGATCGTAACTGA
CCCCCTGGAGCAGGAAGTTAACAAAAACGTGTTCATGGGCAAACTGCATGACATGGTTAACTGGGGCCGTAAAAACTCAA
TTTGGCCCTACAACTTCGGCCTTTCTTGCTGCTATGTAGAGATGGTGACCTCTTTTACCGCAGTGCATGACGTCGCCCGT
TTCGGCGCGGAAGTGTTGCGTGCGTCGCCACGTCAGGCTGACCTGATGGTGGTGGCCGGTACCTGCTTTACCAAAATGGC
CCCGGTTATTCAGCGTCTGTATGACCAAATGCTGGAGCCAAAATGGGTCATTTCTATGGGCGCCTGCGCCAACTCCGGCG
GCATGTACGATATTTATTCGGTAGTGCAGGGCGTTGATAAGTTTATCCCGGTGGATGTGTATATCCCTGGCTGTCCGCCG
CGTCCGGAAGCGTATATGCAGGCGTTGATGCTGCTGCAAGAATCGATTGGCAAAGAGCGCCGTCCGCTCTCATGGGTGGT
GGGCGATCAGGGCGTGTATCGCGCCAATATGCAGCCAGAACGCGAACGTAAACGCGGCGAGCGTATTGCCGTCACGAACC
TGCGTACCCCGGACGAGATTTAA

Upstream 100 bases:

>100_bases
TGGCGCGTTGGACTGGACGCCCGCGCGTTCACGCCGCGAGCGTATGAACCCGGAAACGAACAGTATCGCTAATCGTCAAC
GCTAACCGCGAGGCATTAAG

Downstream 100 bases:

>100_bases
TTTGCGCCTGTCGGCAGCAGCACTTCACTTCGCTAACCACTATTTTAATAGCGCGAAGCACTGCCGACCACCACGGACCA
TTTGCAATGGTGAACAATAT

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLHDMVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVAR
FGAEVLRASPRQADLMVVAGTCFTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQPERERKRGERIAVTNLRTPDEI

Sequences:

>Translated_220_residues
MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLHDMVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVAR
FGAEVLRASPRQADLMVVAGTCFTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQPERERKRGERIAVTNLRTPDEI
>Mature_220_residues
MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLHDMVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVAR
FGAEVLRASPRQADLMVVAGTCFTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQPERERKRGERIAVTNLRTPDEI

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=148, Percent_Identity=54.0540540540541, Blast_Score=174, Evalue=5e-44,
Organism=Escherichia coli, GI1788624, Length=220, Percent_Identity=99.0909090909091, Blast_Score=455, Evalue=1e-129,
Organism=Escherichia coli, GI1789074, Length=122, Percent_Identity=41.8032786885246, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=40.1639344262295, Blast_Score=91, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=148, Percent_Identity=52.027027027027, Blast_Score=165, Evalue=2e-41,
Organism=Drosophila melanogaster, GI18859983, Length=148, Percent_Identity=53.3783783783784, Blast_Score=174, Evalue=6e-44,
Organism=Drosophila melanogaster, GI24642371, Length=148, Percent_Identity=53.3783783783784, Blast_Score=174, Evalue=6e-44,
Organism=Drosophila melanogaster, GI24651058, Length=147, Percent_Identity=53.0612244897959, Blast_Score=170, Evalue=8e-43,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 25089; Mature: 25089

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLHDMVNWGRKNSIWPYNFG
CCCEEEEECCCCCCCCCCCCHHHHHCCHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCC
LSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTCFTKMAPVIQRLYDQMLEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCC
KWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPPRPEAYMQALMLLQESIGKER
CEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCC
RPLSWVVGDQGVYRANMQPERERKRGERIAVTNLRTPDEI
CCCEEEECCCCEEECCCCCHHHHHCCCEEEEECCCCCCCC
>Mature Secondary Structure
MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLHDMVNWGRKNSIWPYNFG
CCCEEEEECCCCCCCCCCCCHHHHHCCHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCC
LSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTCFTKMAPVIQRLYDQMLEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCC
KWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPPRPEAYMQALMLLQESIGKER
CEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCC
RPLSWVVGDQGVYRANMQPERERKRGERIAVTNLRTPDEI
CCCEEEECCCCEEECCCCCHHHHHCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA