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The map label for this gene is glyS [H]

Identifier: 194736702

GI number: 194736702

Start: 3717964

End: 3720033

Strand: Reverse

Name: glyS [H]

Synonym: SeSA_A3849

Alternate gene names: 194736702

Gene position: 3720033-3717964 (Counterclockwise)

Preceding gene: 194737193

Following gene: 194736534

Centisome position: 79.0

GC content: 57.1

Gene sequence:

>2070_bases
ATGTCTGAGAAAACTTTTCTGGTGGAAATCGGCACTGAAGAGCTGCCACCAAAAGCCCTGCGCAGTTTGGCTGAGTCCTT
TGCTGCGAACTTCACTGCGGAGCTGGATAACGCCGGCCTCGCGCACGGTAACGTTGAATGGTTTGCCGCCCCGCGCCGTC
TGGCGCTGAAAGTGGCGAACCTTGCTGAATCTCAGCCCGATCGCGAAGTCGAAAAACGTGGTCCGGCGATTGCCCAGGCG
TTCGACGCCGAAGGCAAACCGAGCAAAGCGGCAGAAGGCTGGGCGCGCGGCTGCGGGATCACCGTTGATCAGGCCGAGCG
TCTGAAGACCGACAAAGGCGAATGGCTGCTGTATCGCGCCCACGTGAAGGGCGAAAGTACCGAAGCGCTGGTGCCGAACA
TGGTAGCCACCTCGCTGGCGAAACTACCGATCCCGAAACTGATGCGCTGGGGCGCGTCTGACGTCCACTTCGTGCGTCCG
GTGCACACCGTGACCCTGCTGTTGGGCGATAAGGTCATTCCGGCGACCATCCTGGGCATTCAGTCTGACCGCGTCATTCG
CGGCCACCGCTTTATGGGTGAGCCTGAGTTCACTATCGACAATGCTGACCAGTACCCGCAAATTCTGCTGGAGCGCGGTA
AAGTCATTGCGGATTACGAAGCGCGTAAAGCCAAAATCAAAGCGGACGCCGAAGAAGCAGCACGTAAGATTGGCGGTAAT
GCCGACCTGAGCGAAAGCCTGCTGGAAGAAGTCGCCTCGCTGGTGGAATGGCCGGTGGTATTGACGGCGAAATTCGAAGA
GAAATTCCTCGCCGTGCCTGCCGAAGCGCTGGTGTACACCATGAAGGGCGACCAGAAGTATTTCCCGGTTTATGACAACG
CGGGCAAGCTGCTGCCAAACTTTATCTTCGTCGCGAACATCGAATCGAAAGATCCGACCCAGATTATCTCCGGTAACGAG
AAAGTGGTGCGTCCGCGTCTGGCGGATGCCGAATTCTTCTTCAATACCGACCGTAAAAAACGTCTGGAAGATCATCTGCC
GCGTCTGCAAACCGTGCTGTTTCAACAGCAGTTGGGTACCCTGCGCGATAAGACCGATCGCATTCAGGCACTGGCAGGCT
GGATTGCCGGTCAGATTGGCGCTGACGTTAACCACGCCACTCGTGCGGGCCTGCTCTCCAAGTGCGACCTGATGACCAAC
ATGGTCTTCGAGTTCACCGACACCCAGGGTGTGATGGGGATGCACTACGCGCGTCACGATGGTGAAGCGGAAGATGTCGC
TGTGGCGCTGAACGAGCAGTATCAGCCGCGCTTTGCCGGTGATGACCTGCCGTCTAACCCGGTGGCCTGTGCGTTGGCGA
TCGCCGATAAGATGGACACTCTGGCGGGTATCTTCGGTATCGGTCAGCATCCGAAAGGCGACAAAGACCCGTTTGCGCTG
CGTCGTGCCGCGCTGGGCGTGCTGCGCATCATTGTTGAGAAGAACCTCAACCTTGATCTGCAAACGCTGACCGAAGAAGC
GGCGCGTCTGTATGGCGACAAGCTGACCAATGCTAACGTAGTCGATGATGTGATCGATTTTATGCTGGGTCGCTTCCGCG
CCTGGTATCAGGACGAAGGTTACACCGTCGACACCATCCAGGCGGTACTGGCGCGTCGTCCGACCCGTCCGGCAGATTTC
GATGCTCGTATGAAAGCGGTATCGCACTTCCGCACCCTGGAAGAAGCCTCTGCGCTGGCGGCGGCGAACAAGCGCGTCTC
CAACATCCTGGCGAAAGCCACTGAGCCGCTGAACGATATCGTCCACGCCTCCGTACTGAAAGAAGCGGCGGAAATTGAGT
TGGCCAGACATCTGGTTGTGCTGCGTGATAAGCTTCAGCCGTACTTTGCCGATGGTCGTTATCAGGAAGCGTTGATTGAG
CTGGCTGCTCTGCGCGCGCCGGTGGATGAATTCTTCGAAAATGTTATGGTTAACGCGGAAGAGAAAGATATCCGTATCAA
CCGCCTGACGTTGTTGTCGAAACTACGCGAACTGTTCCTGCAGGTTGCGGATATTTCGTTACTGCAATAA

Upstream 100 bases:

>100_bases
GCGTATTCGCACCCTGACCAAAGCAGTGGCAGAGGCTTATTATGCTTCCCGTGAAGCCCTCGGCTTCCCGATGTGCAACA
AAGACAAGTAAGAGGCGGCC

Downstream 100 bases:

>100_bases
GCGTTCAGTTAAATAAACAAAAACCTGCTTTCGGGCAGGTTTTTTTATAATATAAATAGTATCAGTCGATTCGCTAATAA
TTCTGTAACTCATAGACGCG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 689; Mature: 688

Protein sequence:

>689_residues
MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGNVEWFAAPRRLALKVANLAESQPDREVEKRGPAIAQA
FDAEGKPSKAAEGWARGCGITVDQAERLKTDKGEWLLYRAHVKGESTEALVPNMVATSLAKLPIPKLMRWGASDVHFVRP
VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPQILLERGKVIADYEARKAKIKADAEEAARKIGGN
ADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEALVYTMKGDQKYFPVYDNAGKLLPNFIFVANIESKDPTQIISGNE
KVVRPRLADAEFFFNTDRKKRLEDHLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAGQIGADVNHATRAGLLSKCDLMTN
MVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVACALAIADKMDTLAGIFGIGQHPKGDKDPFAL
RRAALGVLRIIVEKNLNLDLQTLTEEAARLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADF
DARMKAVSHFRTLEEASALAAANKRVSNILAKATEPLNDIVHASVLKEAAEIELARHLVVLRDKLQPYFADGRYQEALIE
LAALRAPVDEFFENVMVNAEEKDIRINRLTLLSKLRELFLQVADISLLQ

Sequences:

>Translated_689_residues
MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGNVEWFAAPRRLALKVANLAESQPDREVEKRGPAIAQA
FDAEGKPSKAAEGWARGCGITVDQAERLKTDKGEWLLYRAHVKGESTEALVPNMVATSLAKLPIPKLMRWGASDVHFVRP
VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPQILLERGKVIADYEARKAKIKADAEEAARKIGGN
ADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEALVYTMKGDQKYFPVYDNAGKLLPNFIFVANIESKDPTQIISGNE
KVVRPRLADAEFFFNTDRKKRLEDHLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAGQIGADVNHATRAGLLSKCDLMTN
MVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVACALAIADKMDTLAGIFGIGQHPKGDKDPFAL
RRAALGVLRIIVEKNLNLDLQTLTEEAARLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADF
DARMKAVSHFRTLEEASALAAANKRVSNILAKATEPLNDIVHASVLKEAAEIELARHLVVLRDKLQPYFADGRYQEALIE
LAALRAPVDEFFENVMVNAEEKDIRINRLTLLSKLRELFLQVADISLLQ
>Mature_688_residues
SEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGNVEWFAAPRRLALKVANLAESQPDREVEKRGPAIAQAF
DAEGKPSKAAEGWARGCGITVDQAERLKTDKGEWLLYRAHVKGESTEALVPNMVATSLAKLPIPKLMRWGASDVHFVRPV
HTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPQILLERGKVIADYEARKAKIKADAEEAARKIGGNA
DLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEALVYTMKGDQKYFPVYDNAGKLLPNFIFVANIESKDPTQIISGNEK
VVRPRLADAEFFFNTDRKKRLEDHLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAGQIGADVNHATRAGLLSKCDLMTNM
VFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVACALAIADKMDTLAGIFGIGQHPKGDKDPFALR
RAALGVLRIIVEKNLNLDLQTLTEEAARLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFD
ARMKAVSHFRTLEEASALAAANKRVSNILAKATEPLNDIVHASVLKEAAEIELARHLVVLRDKLQPYFADGRYQEALIEL
AALRAPVDEFFENVMVNAEEKDIRINRLTLLSKLRELFLQVADISLLQ

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=689, Percent_Identity=92.7431059506531, Blast_Score=1297, Evalue=0.0,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 76468; Mature: 76337

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGNVEWFAAPRRLALKVAN
CCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEECCHHHHHHHHH
LAESQPDREVEKRGPAIAQAFDAEGKPSKAAEGWARGCGITVDQAERLKTDKGEWLLYRA
HHCCCCCHHHHHCCCHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEE
HVKGESTEALVPNMVATSLAKLPIPKLMRWGASDVHFVRPVHTVTLLLGDKVIPATILGI
EECCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEECHHHHHHHHHCCCCHHHHHEEC
QSDRVIRGHRFMGEPEFTIDNADQYPQILLERGKVIADYEARKAKIKADAEEAARKIGGN
CCCCEEECCCCCCCCCCEECCCHHHHHHHHHCCCEEECCHHHHHHHCCCHHHHHHHCCCC
ADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEALVYTMKGDQKYFPVYDNAGKLLPN
CCHHHHHHHHHHHHHHCCEEEEEEHHHHHHHCCHHHEEEEECCCCEECCEECCCCHHCCC
FIFVANIESKDPTQIISGNEKVVRPRLADAEFFFNTDRKKRLEDHLPRLQTVLFQQQLGT
EEEEEECCCCCCHHHHCCCCCEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LRDKTDRIQALAGWIAGQIGADVNHATRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHCCC
GEAEDVAVALNEQYQPRFAGDDLPSNPVACALAIADKMDTLAGIFGIGQHPKGDKDPFAL
CCHHHEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAALGVLRIIVEKNLNLDLQTLTEEAARLYGDKLTNANVVDDVIDFMLGRFRAWYQDEG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
YTVDTIQAVLARRPTRPADFDARMKAVSHFRTLEEASALAAANKRVSNILAKATEPLNDI
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHASVLKEAAEIELARHLVVLRDKLQPYFADGRYQEALIELAALRAPVDEFFENVMVNAE
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCC
EKDIRINRLTLLSKLRELFLQVADISLLQ
HHCEEHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGNVEWFAAPRRLALKVAN
CCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEECCHHHHHHHHH
LAESQPDREVEKRGPAIAQAFDAEGKPSKAAEGWARGCGITVDQAERLKTDKGEWLLYRA
HHCCCCCHHHHHCCCHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEE
HVKGESTEALVPNMVATSLAKLPIPKLMRWGASDVHFVRPVHTVTLLLGDKVIPATILGI
EECCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEECHHHHHHHHHCCCCHHHHHEEC
QSDRVIRGHRFMGEPEFTIDNADQYPQILLERGKVIADYEARKAKIKADAEEAARKIGGN
CCCCEEECCCCCCCCCCEECCCHHHHHHHHHCCCEEECCHHHHHHHCCCHHHHHHHCCCC
ADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEALVYTMKGDQKYFPVYDNAGKLLPN
CCHHHHHHHHHHHHHHCCEEEEEEHHHHHHHCCHHHEEEEECCCCEECCEECCCCHHCCC
FIFVANIESKDPTQIISGNEKVVRPRLADAEFFFNTDRKKRLEDHLPRLQTVLFQQQLGT
EEEEEECCCCCCHHHHCCCCCEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LRDKTDRIQALAGWIAGQIGADVNHATRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHCCC
GEAEDVAVALNEQYQPRFAGDDLPSNPVACALAIADKMDTLAGIFGIGQHPKGDKDPFAL
CCHHHEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAALGVLRIIVEKNLNLDLQTLTEEAARLYGDKLTNANVVDDVIDFMLGRFRAWYQDEG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
YTVDTIQAVLARRPTRPADFDARMKAVSHFRTLEEASALAAANKRVSNILAKATEPLNDI
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHASVLKEAAEIELARHLVVLRDKLQPYFADGRYQEALIELAALRAPVDEFFENVMVNAE
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCC
EKDIRINRLTLLSKLRELFLQVADISLLQ
HHCEEHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA