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The map label for this gene is dusA [H]

Identifier: 194736682

GI number: 194736682

Start: 4354476

End: 4355513

Strand: Direct

Name: dusA [H]

Synonym: SeSA_A4491

Alternate gene names: 194736682

Gene position: 4354476-4355513 (Clockwise)

Preceding gene: 194734225

Following gene: 194734595

Centisome position: 92.47

GC content: 53.56

Gene sequence:

>1038_bases
ATGCACGATAATCATGAAACAAAAAAAATTAATCAAACCAGCGTGATGCCTGAAAAAACTGGTGTTTACTGGAATTCTCG
GTTTAGCATTGCTCCTATGCTCGACTGGACGGACAGACATTGCCGCTATTTCCTGCGTTTGCTGTCTCGCCAGACGCTGC
TCTACACCGAAATGGTGACCACGGGCGCGATTATTCACGGTAAGGGTGACTATCTGGCTTACAGCGAAGAAGAGCATCCG
GTCGCTCTACAGCTTGGTGGAAGCGATCCGGCTCAGCTTGCGCATTGTGCAAAGCTGGCGGAAGCGCGTGGCTACGATGA
AATTAACCTCAACGTGGGGTGCCCCTCCGATCGCGTGCAAAACGGTATGTTTGGCGCCTGTTTGATGGGCAATGCGCAAC
TGGTCGCCGATTGTGTTAAAGCCATGCGTGATGTCGTCTCGATTCCGGTAACGGTAAAAACCCGCATTGGTATTGACGAT
CAGGACAGCTATGCGTTTCTGTGTGATTTCATCAATACGGTTTCCGGTCAGGGCGAATGCGAGATGTTTATTATCCATGC
GCGCAAAGCTTGGCTTTCTGGCTTAAGCCCGAAAGAAAATCGTGAGATCCCGCCGCTGGATTACCCGCGCGTCTATCAGC
TAAAGCGGGATTTTCCGCACCTGACCATGTCCATTAACGGCGGCATCAAATCGCTGGAAGAGGCGAAGGAGCACCTGCGC
CATATGGATGGCGTCATGGTTGGCCGCGAAGCTTATCAGAATCCGGGTATACTGGCCGCGGTGGATCGGGAGATCTTTGG
CGCCGATACCACCGATGCCGACCCGGTTACGGTGGTTCGCGCGATGTATCCCTATATTGAGCGTGAATTGAGCCAGGGAG
CGTATCTGGGGCATATCACTCGCCATATGCTGGGGTTGTTCCAGGGCATCCCCGGCGCGCGGCAGTGGCGTCGCTATCTG
AGCGAAAACGCCCATAAAGCTGGCGCGGATGTTGCTGTACTGGAGCAGGCGCTAAAACTGGTAGCAGACAAGCGTTAA

Upstream 100 bases:

>100_bases
ATAGTACGCCATTACACGACCTCGTTATTTCGGGTTCCCATAAAACGTGGGAACCTGTGCGGGAACCTAACGCGAGAAAA
ATAGCCTGAAATGTTCAAAA

Downstream 100 bases:

>100_bases
AAGTTCGCCAAAAATTAGTCAATCTCACCACGCTCTGTGCAGTCTTGCAGGGCGTTTTCTTTATATATCAAAAACATAAA
TATTGGCATGATTTTTGTAA

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MHDNHETKKINQTSVMPEKTGVYWNSRFSIAPMLDWTDRHCRYFLRLLSRQTLLYTEMVTTGAIIHGKGDYLAYSEEEHP
VALQLGGSDPAQLAHCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
QDSYAFLCDFINTVSGQGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKEHLR
HMDGVMVGREAYQNPGILAAVDREIFGADTTDADPVTVVRAMYPYIERELSQGAYLGHITRHMLGLFQGIPGARQWRRYL
SENAHKAGADVAVLEQALKLVADKR

Sequences:

>Translated_345_residues
MHDNHETKKINQTSVMPEKTGVYWNSRFSIAPMLDWTDRHCRYFLRLLSRQTLLYTEMVTTGAIIHGKGDYLAYSEEEHP
VALQLGGSDPAQLAHCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
QDSYAFLCDFINTVSGQGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKEHLR
HMDGVMVGREAYQNPGILAAVDREIFGADTTDADPVTVVRAMYPYIERELSQGAYLGHITRHMLGLFQGIPGARQWRRYL
SENAHKAGADVAVLEQALKLVADKR
>Mature_345_residues
MHDNHETKKINQTSVMPEKTGVYWNSRFSIAPMLDWTDRHCRYFLRLLSRQTLLYTEMVTTGAIIHGKGDYLAYSEEEHP
VALQLGGSDPAQLAHCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
QDSYAFLCDFINTVSGQGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKEHLR
HMDGVMVGREAYQNPGILAAVDREIFGADTTDADPVTVVRAMYPYIERELSQGAYLGHITRHMLGLFQGIPGARQWRRYL
SENAHKAGADVAVLEQALKLVADKR

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=308, Percent_Identity=23.7012987012987, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI145693211, Length=330, Percent_Identity=93.9393939393939, Blast_Score=654, Evalue=0.0,
Organism=Escherichia coli, GI1788462, Length=251, Percent_Identity=24.7011952191235, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=245, Percent_Identity=26.530612244898, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24580595, Length=309, Percent_Identity=22.9773462783171, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI19920448, Length=309, Percent_Identity=22.9773462783171, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 38654; Mature: 38654

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDNHETKKINQTSVMPEKTGVYWNSRFSIAPMLDWTDRHCRYFLRLLSRQTLLYTEMVT
CCCCCCCCCCCHHCCCCCCCCCEECCCEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHH
TGAIIHGKGDYLAYSEEEHPVALQLGGSDPAQLAHCAKLAEARGYDEINLNVGCPSDRVQ
HCEEEECCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHC
NGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYAFLCDFINTVSGQGEC
CCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCE
EMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKEHLR
EEEEEEEHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHH
HMDGVMVGREAYQNPGILAAVDREIFGADTTDADPVTVVRAMYPYIERELSQGAYLGHIT
HHHHHHCCHHHHCCCCEEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHH
RHMLGLFQGIPGARQWRRYLSENAHKAGADVAVLEQALKLVADKR
HHHHHHHHCCCCHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHDNHETKKINQTSVMPEKTGVYWNSRFSIAPMLDWTDRHCRYFLRLLSRQTLLYTEMVT
CCCCCCCCCCCHHCCCCCCCCCEECCCEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHH
TGAIIHGKGDYLAYSEEEHPVALQLGGSDPAQLAHCAKLAEARGYDEINLNVGCPSDRVQ
HCEEEECCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHC
NGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYAFLCDFINTVSGQGEC
CCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCE
EMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKEHLR
EEEEEEEHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHH
HMDGVMVGREAYQNPGILAAVDREIFGADTTDADPVTVVRAMYPYIERELSQGAYLGHIT
HHHHHHCCHHHHCCCCEEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHH
RHMLGLFQGIPGARQWRRYLSENAHKAGADVAVLEQALKLVADKR
HHHHHHHHCCCCHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]