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The map label for this gene is rlmF [H]

Identifier: 194736599

GI number: 194736599

Start: 946789

End: 947715

Strand: Direct

Name: rlmF [H]

Synonym: SeSA_A0974

Alternate gene names: 194736599

Gene position: 946789-947715 (Clockwise)

Preceding gene: 194734658

Following gene: 194734266

Centisome position: 20.11

GC content: 54.48

Gene sequence:

>927_bases
ATGTCCGCCCAGAAATCGGGGTTACACCCGCGCAACCGCCATCAACACCGTTATGATCTCGCCGCACTGTGTCAGACCAC
GCCGGAATTGACGTCCTTTCTTATCCGCACGCCCGCCGGTGAACAAAGCGTTGATTTTGCTAATCCGCAAGCGGTAAAAG
CGCTCAATAAGGCGTTGCTGGCGCATTTTTATGCCGTCACACACTGGGACATTCCGCCAGGGTTTTTATGTCCGCCAGTG
CCTGGCCGTGCGGATTATATCCATCATCTGGCGGATCTGCTTGGCGAAACGACAGGGAGTATTCCGGCGCAGGCAACTAT
TCTGGACGTGGGCGTGGGCGCCAATTGTATTTATCCGCTTATCGGCGTTCATGAATATGGCTGGCGATTTACCGGTAGTG
AAGTCAGCGACGCAGCGATGTCCAGCGCGCAGGCTATCATTCAGGCTAATACGGGGTTAAGCCGCGCCATTCGTCTGCGT
CGGCAAAAAGATCCGGCGGCCATTTTTACCGGTATCATTCATAAAAATGAATTTTATGACGCAACGCTTTGTAACCCGCC
TTTTCACGACTCCGCCGCCGCAGCTCGCGCGGGCAGCGAGCGTAAGCGCCGCAATTTGGGGCAGAATAAAGATGATGCGC
TGAACTTTGGCGGTCAACAGCAGGAGCTATGGTGCGAAGGCGGCGAAGTCGCCTTTATTAAAAAAATGATTGCTGAAAGC
CAATCCTTCCGCCGTCAGGTGTTGTGGTTTACGACGTTGGTGTCGCGCGGCGAGAATTTACCGCCGCTTTACCGGGCGTT
GACGGAGGCTGGCGCGGTGAAAGTGGTGAAAAAAGAGATGGCCCAGGGGCAAAAGCAAAGCCGCTTTATTGCCTGGACCT
TTATGGATGACGACCAGCGTCGCCGTTTTATCACCCGCAAACGCTAA

Upstream 100 bases:

>100_bases
CAGATATCCGATCAAACGAGCGAGGCAGGGAATTCGCGGTGGTATATTTACCCCGTGGCGTTATACGGGTATCTTACGCC
GCTGCTAAAAGGAGAATGCC

Downstream 100 bases:

>100_bases
ATCGTCGGTTCGCGCGGCGGCAGCGAATCAGACGACGGGCCGCCGGTGGGGGCGGGCAGAACCTGATACGTTTGCACCGG
CGCGCGCACATTAGCCAGAT

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSAQKSGLHPRNRHQHRYDLAALCQTTPELTSFLIRTPAGEQSVDFANPQAVKALNKALLAHFYAVTHWDIPPGFLCPPV
PGRADYIHHLADLLGETTGSIPAQATILDVGVGANCIYPLIGVHEYGWRFTGSEVSDAAMSSAQAIIQANTGLSRAIRLR
RQKDPAAIFTGIIHKNEFYDATLCNPPFHDSAAAARAGSERKRRNLGQNKDDALNFGGQQQELWCEGGEVAFIKKMIAES
QSFRRQVLWFTTLVSRGENLPPLYRALTEAGAVKVVKKEMAQGQKQSRFIAWTFMDDDQRRRFITRKR

Sequences:

>Translated_308_residues
MSAQKSGLHPRNRHQHRYDLAALCQTTPELTSFLIRTPAGEQSVDFANPQAVKALNKALLAHFYAVTHWDIPPGFLCPPV
PGRADYIHHLADLLGETTGSIPAQATILDVGVGANCIYPLIGVHEYGWRFTGSEVSDAAMSSAQAIIQANTGLSRAIRLR
RQKDPAAIFTGIIHKNEFYDATLCNPPFHDSAAAARAGSERKRRNLGQNKDDALNFGGQQQELWCEGGEVAFIKKMIAES
QSFRRQVLWFTTLVSRGENLPPLYRALTEAGAVKVVKKEMAQGQKQSRFIAWTFMDDDQRRRFITRKR
>Mature_307_residues
SAQKSGLHPRNRHQHRYDLAALCQTTPELTSFLIRTPAGEQSVDFANPQAVKALNKALLAHFYAVTHWDIPPGFLCPPVP
GRADYIHHLADLLGETTGSIPAQATILDVGVGANCIYPLIGVHEYGWRFTGSEVSDAAMSSAQAIIQANTGLSRAIRLRR
QKDPAAIFTGIIHKNEFYDATLCNPPFHDSAAAARAGSERKRRNLGQNKDDALNFGGQQQELWCEGGEVAFIKKMIAESQ
SFRRQVLWFTTLVSRGENLPPLYRALTEAGAVKVVKKEMAQGQKQSRFIAWTFMDDDQRRRFITRKR

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA [H]

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family [H]

Homologues:

Organism=Homo sapiens, GI122114654, Length=299, Percent_Identity=31.7725752508361, Blast_Score=156, Evalue=2e-38,
Organism=Escherichia coli, GI87081786, Length=308, Percent_Identity=83.7662337662338, Blast_Score=551, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI32564931, Length=308, Percent_Identity=27.5974025974026, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32564933, Length=308, Percent_Identity=26.6233766233766, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI281363415, Length=291, Percent_Identity=28.5223367697594, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI19922302, Length=291, Percent_Identity=28.5223367697594, Blast_Score=117, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016909
- InterPro:   IPR010286 [H]

Pfam domain/function: PF05971 Methyltransf_10 [H]

EC number: =2.1.1.181 [H]

Molecular weight: Translated: 34300; Mature: 34169

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQKSGLHPRNRHQHRYDLAALCQTTPELTSFLIRTPAGEQSVDFANPQAVKALNKALL
CCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
AHFYAVTHWDIPPGFLCPPVPGRADYIHHLADLLGETTGSIPAQATILDVGVGANCIYPL
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHH
IGVHEYGWRFTGSEVSDAAMSSAQAIIQANTGLSRAIRLRRQKDPAAIFTGIIHKNEFYD
HHHHHCCCEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCC
ATLCNPPFHDSAAAARAGSERKRRNLGQNKDDALNFGGQQQELWCEGGEVAFIKKMIAES
CCCCCCCCCCCHHHHHCCHHHHHHHCCCCCCHHHCCCCCCHHHHCCCCCHHHHHHHHHHH
QSFRRQVLWFTTLVSRGENLPPLYRALTEAGAVKVVKKEMAQGQKQSRFIAWTFMDDDQR
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCEEEEEEEECCHHH
RRFITRKR
HHHHCCCC
>Mature Secondary Structure 
SAQKSGLHPRNRHQHRYDLAALCQTTPELTSFLIRTPAGEQSVDFANPQAVKALNKALL
CCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
AHFYAVTHWDIPPGFLCPPVPGRADYIHHLADLLGETTGSIPAQATILDVGVGANCIYPL
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHH
IGVHEYGWRFTGSEVSDAAMSSAQAIIQANTGLSRAIRLRRQKDPAAIFTGIIHKNEFYD
HHHHHCCCEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCC
ATLCNPPFHDSAAAARAGSERKRRNLGQNKDDALNFGGQQQELWCEGGEVAFIKKMIAES
CCCCCCCCCCCHHHHHCCHHHHHHHCCCCCCHHHCCCCCCHHHHCCCCCHHHHHHHHHHH
QSFRRQVLWFTTLVSRGENLPPLYRALTEAGAVKVVKKEMAQGQKQSRFIAWTFMDDDQR
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCEEEEEEEECCHHH
RRFITRKR
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA