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The map label for this gene is glgX

Identifier: 194736583

GI number: 194736583

Start: 3588042

End: 3590018

Strand: Reverse

Name: glgX

Synonym: SeSA_A3727

Alternate gene names: 194736583

Gene position: 3590018-3588042 (Counterclockwise)

Preceding gene: 194737044

Following gene: 194735772

Centisome position: 76.24

GC content: 57.11

Gene sequence:

>1977_bases
ATGACGCAGCTTGCGATCGGCGAAGCAACGCCGCATGGCGCAACGTATGACGGTCATGGCGTGAATTTCACGCTCTTTTC
CGCCCATGCGGAGCGCGTAGAGCTGTGCGTTTTTGATTCCCGAGGGAATGAGCGCCGCTATGACCTGCCAGGACGTCGCG
GCGATGTCTGGCATGGTTATCTGGCAGGCGCCAGGCCTGGTTTGCGCTATGGCTATCGCGTACATGGCCCGTGGCAGCCA
GCGCAGGGGCATCGGTTTAATCCGGCGAAGTTACTGCTTGATCCTTATGCGCGCCGGGTGGAGGGCGAACTAAAAGATCA
TCCGCTGTTGCACGGCGGACACGATGAACCGGATTACCGCGATAACGCGGCGGTGGCGCCGAAAAGCGTGATAATCAGCG
ACCATTATGACTGGGAAGATGACGCCGCGCCGCGCACGCCGTGGGGAAAAACGGTCATCTATGAAGCGCATGTCAAAGGG
CTTACCTACCTGCATCCGGAACTCCCCCAGGAGATACGCGGCACCTATAAAGCGCTCGGTCATCCGGTCATGGTGGCGTA
TTTCAAACAGCTTGGCATTACGGCGCTGGAACTGTTGCCGGTGGCGCAGTTTGCCAGCGAGCCGCGCCTGCAACGTATGG
GACTGACCAATTATTGGGGTTATAACCCGATGGCGATGTTCGCGCTGCATCCCGCCTGGGCCAGTTCGCCCGAGACGGCG
CTGGACGAGTTTCGCGACGCGGTGAAGGCGCTGCATCGCGCGGGGATTGAGGTCATTCTGGACATCGTATTAAACCATAG
CGCCGAGCTGGATCTGGACGGTCCAACCTTCTCCCTGCGCGGAATTGATAACCGTAGCTATTATTGGATAAGGGACGATG
GCGATTATCACAACTGGACGGGGTGCGGCAACACGCTCAATTTAAGCCATCCTGGCGTGGTGGAATACGCGTGCGAGTGT
TTGCGCTATTGGGTGGAAACCTGTCATGTCGATGGTTTTCGTTTTGATTTGGCGTCCGTCATGGGGCGCACGCCGACGTT
TCGCCAGGATGCGCCGCTGTTCGCCGCGATTAAAGCGTGTCCTGTGTTATCAACGGTCAAGCTGATTGCTGAACCGTGGG
ATATTGGCGAGGGCGGCTATCAGGTCGGGAATTTTCCGCCACCGTTTGCCGAGTGGAACGATCATTTTCGCGATGCGGCC
CGCCGATTCTGGTTGCCGCGTAACCTGACGACGGGGGAATTTGCCTGTCGTTTCGCCGCTTCCAGCGATGTGTTTAAACG
CAATGGCCGCACGCCCGGCGCCTCTGTCAATCTGCTCACGGCGCATGACGGTTTTACGCTCCGGGACTGCGTCTGTTTCA
ATCAGAAACACAATGAGGCGAACGGTGAGGAAAATCGCGACGGCACCAACAGCAATTACAGCGACAATCATGGTAAAGAA
GGATTAGGCGGTCCGCTTGATTTAATGGAGCGGCGGCGCGACAGCATTCATGCCCTGCTGGCGACGCTACTGCTCTCTCA
GGGAACGCCGATGCTGCTGGCAGGCGATGAACATGGCCATAGCCAGCACGGCAACAATAACGCCTACTGTCAGGATAATG
CCTTAACCTGGCTGGACTGGCAGCAGGCAAACCGTGGGTTAACCACGTTTACCGCCGCGCTGATTCGTTTGCGTCAGCAG
ATACCGGCTTTAACCGGCAATAGCTGGTGGGAAGAAGGCGATGGCAACGTGCGTTGGCTGAATAAAAACGCGCAACCCTT
GAGTGCGGATGAGTGGCAAAACGGGCCTAAGCTGATGCAAATTCTGTTGTCGGACCGTTTTCTGATTGCGATAAATGCCA
CGCTTGAGGTGACAGATATAGTTTTACCCGAAGGGGAATGGCGCGCCGTTCCCCCATTCGCTGGAGAGGATAATCCGGTG
ATAACGGCGGTCTGGCAGGGACCTGCGCATGGACTGTGTGTGTTCCAGAGAGGATAA

Upstream 100 bases:

>100_bases
GCGTGGTGCACAGCGATGAGATCGAAAGCCATGGCCGTCAGCACTCTCTTAGCCTGACGTTACCGCCGTTGGCGACGATC
TGGCTGATGCGGGAGGGGGA

Downstream 100 bases:

>100_bases
AAAAGGAGTTAATCATGGTGAGTTTAGAGAAGAACGATCGTGTAATGTTGGCGCGCCAGCTGCCACTGAAATCTGTTGCC
CTAATCCTGGCCGGCGGCCG

Product: glycogen debranching enzyme

Products: NA

Alternate protein names: Glycogen operon protein GlgX

Number of amino acids: Translated: 658; Mature: 657

Protein sequence:

>658_residues
MTQLAIGEATPHGATYDGHGVNFTLFSAHAERVELCVFDSRGNERRYDLPGRRGDVWHGYLAGARPGLRYGYRVHGPWQP
AQGHRFNPAKLLLDPYARRVEGELKDHPLLHGGHDEPDYRDNAAVAPKSVIISDHYDWEDDAAPRTPWGKTVIYEAHVKG
LTYLHPELPQEIRGTYKALGHPVMVAYFKQLGITALELLPVAQFASEPRLQRMGLTNYWGYNPMAMFALHPAWASSPETA
LDEFRDAVKALHRAGIEVILDIVLNHSAELDLDGPTFSLRGIDNRSYYWIRDDGDYHNWTGCGNTLNLSHPGVVEYACEC
LRYWVETCHVDGFRFDLASVMGRTPTFRQDAPLFAAIKACPVLSTVKLIAEPWDIGEGGYQVGNFPPPFAEWNDHFRDAA
RRFWLPRNLTTGEFACRFAASSDVFKRNGRTPGASVNLLTAHDGFTLRDCVCFNQKHNEANGEENRDGTNSNYSDNHGKE
GLGGPLDLMERRRDSIHALLATLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNALTWLDWQQANRGLTTFTAALIRLRQQ
IPALTGNSWWEEGDGNVRWLNKNAQPLSADEWQNGPKLMQILLSDRFLIAINATLEVTDIVLPEGEWRAVPPFAGEDNPV
ITAVWQGPAHGLCVFQRG

Sequences:

>Translated_658_residues
MTQLAIGEATPHGATYDGHGVNFTLFSAHAERVELCVFDSRGNERRYDLPGRRGDVWHGYLAGARPGLRYGYRVHGPWQP
AQGHRFNPAKLLLDPYARRVEGELKDHPLLHGGHDEPDYRDNAAVAPKSVIISDHYDWEDDAAPRTPWGKTVIYEAHVKG
LTYLHPELPQEIRGTYKALGHPVMVAYFKQLGITALELLPVAQFASEPRLQRMGLTNYWGYNPMAMFALHPAWASSPETA
LDEFRDAVKALHRAGIEVILDIVLNHSAELDLDGPTFSLRGIDNRSYYWIRDDGDYHNWTGCGNTLNLSHPGVVEYACEC
LRYWVETCHVDGFRFDLASVMGRTPTFRQDAPLFAAIKACPVLSTVKLIAEPWDIGEGGYQVGNFPPPFAEWNDHFRDAA
RRFWLPRNLTTGEFACRFAASSDVFKRNGRTPGASVNLLTAHDGFTLRDCVCFNQKHNEANGEENRDGTNSNYSDNHGKE
GLGGPLDLMERRRDSIHALLATLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNALTWLDWQQANRGLTTFTAALIRLRQQ
IPALTGNSWWEEGDGNVRWLNKNAQPLSADEWQNGPKLMQILLSDRFLIAINATLEVTDIVLPEGEWRAVPPFAGEDNPV
ITAVWQGPAHGLCVFQRG
>Mature_657_residues
TQLAIGEATPHGATYDGHGVNFTLFSAHAERVELCVFDSRGNERRYDLPGRRGDVWHGYLAGARPGLRYGYRVHGPWQPA
QGHRFNPAKLLLDPYARRVEGELKDHPLLHGGHDEPDYRDNAAVAPKSVIISDHYDWEDDAAPRTPWGKTVIYEAHVKGL
TYLHPELPQEIRGTYKALGHPVMVAYFKQLGITALELLPVAQFASEPRLQRMGLTNYWGYNPMAMFALHPAWASSPETAL
DEFRDAVKALHRAGIEVILDIVLNHSAELDLDGPTFSLRGIDNRSYYWIRDDGDYHNWTGCGNTLNLSHPGVVEYACECL
RYWVETCHVDGFRFDLASVMGRTPTFRQDAPLFAAIKACPVLSTVKLIAEPWDIGEGGYQVGNFPPPFAEWNDHFRDAAR
RFWLPRNLTTGEFACRFAASSDVFKRNGRTPGASVNLLTAHDGFTLRDCVCFNQKHNEANGEENRDGTNSNYSDNHGKEG
LGGPLDLMERRRDSIHALLATLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNALTWLDWQQANRGLTTFTAALIRLRQQI
PALTGNSWWEEGDGNVRWLNKNAQPLSADEWQNGPKLMQILLSDRFLIAINATLEVTDIVLPEGEWRAVPPFAGEDNPVI
TAVWQGPAHGLCVFQRG

Specific function: Hydrolyzes the alpha-1,6-glucosidic linkages in glycogen which has first been partially depolymerized by phosphorylase. Shows only very little activity with native glycogen

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Escherichia coli, GI2367229, Length=657, Percent_Identity=85.2359208523592, Blast_Score=1182, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGX_SALA4 (B5F8Q3)

Other databases:

- EMBL:   CP001138
- RefSeq:   YP_002148460.1
- GeneID:   6794154
- GenomeReviews:   CP001138_GR
- KEGG:   sea:SeAg_B3738
- HOGENOM:   HBG350946
- OMA:   EMALDEF
- ProtClustDB:   PRK03705
- HAMAP:   MF_01248
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR022844
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR02100

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: 3.2.1.- [C]

Molecular weight: Translated: 73656; Mature: 73525

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: ACT_SITE 336-336 ACT_SITE 371-371

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLAIGEATPHGATYDGHGVNFTLFSAHAERVELCVFDSRGNERRYDLPGRRGDVWHGY
CCCEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEE
LAGARPGLRYGYRVHGPWQPAQGHRFNPAKLLLDPYARRVEGELKDHPLLHGGHDEPDYR
CCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCC
DNAAVAPKSVIISDHYDWEDDAAPRTPWGKTVIYEAHVKGLTYLHPELPQEIRGTYKALG
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHC
HPVMVAYFKQLGITALELLPVAQFASEPRLQRMGLTNYWGYNPMAMFALHPAWASSPETA
CHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHH
LDEFRDAVKALHRAGIEVILDIVLNHSAELDLDGPTFSLRGIDNRSYYWIRDDGDYHNWT
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCEECCCCEEEEEECCCCEEEEEECCCCCCCCC
GCGNTLNLSHPGVVEYACECLRYWVETCHVDGFRFDLASVMGRTPTFRQDAPLFAAIKAC
CCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
PVLSTVKLIAEPWDIGEGGYQVGNFPPPFAEWNDHFRDAARRFWLPRNLTTGEFACRFAA
HHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHEEEECC
SSDVFKRNGRTPGASVNLLTAHDGFTLRDCVCFNQKHNEANGEENRDGTNSNYSDNHGKE
CCHHHHHCCCCCCCEEEEEEECCCCEEEHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
GLGGPLDLMERRRDSIHALLATLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNALTWLDW
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEECCCCEEEEEH
QQANRGLTTFTAALIRLRQQIPALTGNSWWEEGDGNVRWLNKNAQPLSADEWQNGPKLMQ
HHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHCCCCHHHHH
ILLSDRFLIAINATLEVTDIVLPEGEWRAVPPFAGEDNPVITAVWQGPAHGLCVFQRG
HHHCCCEEEEEECEEEEEEEEECCCCCEECCCCCCCCCCEEEEEECCCCCCEEEEECC
>Mature Secondary Structure 
TQLAIGEATPHGATYDGHGVNFTLFSAHAERVELCVFDSRGNERRYDLPGRRGDVWHGY
CCEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEE
LAGARPGLRYGYRVHGPWQPAQGHRFNPAKLLLDPYARRVEGELKDHPLLHGGHDEPDYR
CCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCC
DNAAVAPKSVIISDHYDWEDDAAPRTPWGKTVIYEAHVKGLTYLHPELPQEIRGTYKALG
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHC
HPVMVAYFKQLGITALELLPVAQFASEPRLQRMGLTNYWGYNPMAMFALHPAWASSPETA
CHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHH
LDEFRDAVKALHRAGIEVILDIVLNHSAELDLDGPTFSLRGIDNRSYYWIRDDGDYHNWT
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCEECCCCEEEEEECCCCEEEEEECCCCCCCCC
GCGNTLNLSHPGVVEYACECLRYWVETCHVDGFRFDLASVMGRTPTFRQDAPLFAAIKAC
CCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
PVLSTVKLIAEPWDIGEGGYQVGNFPPPFAEWNDHFRDAARRFWLPRNLTTGEFACRFAA
HHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHEEEECC
SSDVFKRNGRTPGASVNLLTAHDGFTLRDCVCFNQKHNEANGEENRDGTNSNYSDNHGKE
CCHHHHHCCCCCCCEEEEEEECCCCEEEHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
GLGGPLDLMERRRDSIHALLATLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNALTWLDW
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEECCCCEEEEEH
QQANRGLTTFTAALIRLRQQIPALTGNSWWEEGDGNVRWLNKNAQPLSADEWQNGPKLMQ
HHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHCCCCHHHHH
ILLSDRFLIAINATLEVTDIVLPEGEWRAVPPFAGEDNPVITAVWQGPAHGLCVFQRG
HHHCCCEEEEEECEEEEEEEEECCCCCEECCCCCCCCCCEEEEEECCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA