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The map label for this gene is nfo [H]

Identifier: 194736559

GI number: 194736559

Start: 2323125

End: 2323982

Strand: Direct

Name: nfo [H]

Synonym: SeSA_A2442

Alternate gene names: 194736559

Gene position: 2323125-2323982 (Clockwise)

Preceding gene: 194736830

Following gene: 194735444

Centisome position: 49.33

GC content: 53.73

Gene sequence:

>858_bases
ATGAAATACATCGGAGCGCACGTCAGCGCTGCTGGCGGTCTGGCTAACGCCCCTGCCCGCGCGGCTGAAATTGGCGCAAC
GGCCTTTGCGCTTTTCACAAAAAACCAGCGTCAGTGGCGTGCCGCCCCCCTTACTCCCCAGGTCATTGATGACTTTAAAA
TCGCCTGTGAAAAGTATCATTTCTCGGCGGCGCAAATTCTTCCCCACGATAGTTACCTGATTAATCTGGGCCATCCGGTC
AGTGAAGCACTGGAAAAATCACGCGTTGCCTTTCTCGATGAAATGCAACGCTGTGAACAACTCGGCTTAACCTTGCTTAA
TTTTCATCCCGGTAGCCATTTGATGCAGATTGCACAGGAGGATTGCCTGGCGCGGATCGCGGAATCCATCAATATTGCCC
TCGCGCAGACCGAGGGCGTTACGGCGGTTATCGAAAATACAGCCGGTCAGGGCAGTAATCTGGGGTTTGAGTTTGAACAG
TTAGCCGCCATCATCGACGGCGTGGAAGATAAGTCGCGCGTTGGCGTCTGTATCGATACCTGCCATGCCTTTGCCGCCGG
ATATGATCTGCGTACGCCAGAGGCGTGCGAAAAAACGTTCGCCGGATTCGGGAAAATTGTCGGATTTCAGTATTTGCGCG
GAATGCACCTGAACGACGCCAAAAGCGCCTTCGGTAGCCGCGTTGACCGCCATCACAGTCTGGGTGAAGGCAATATCGGC
CACGATGCGTTTCGTTGGATTATGCAGGATGCGCGTTTTGACGGTATTCCGCTGATACTGGAGACCATCAATCCTGATAT
CTGGGCGGAAGAGATTGCGTGGTTAAAAGCCCAGCAAATTGCCGAAGCGGTGGCCTGA

Upstream 100 bases:

>100_bases
AATCCATAGCCTTATCGCATAAATTATTACGTTTCACGCCTGTTAACCCGTTATCATAGCGTTCGTAATGAACCGTTAGC
GGGTTTAACAGGAGTCTTTT

Downstream 100 bases:

>100_bases
CGCGCAATGTTCTTCAGGCAATAAAAAAGGCAGAGCGCGCTCTGCCTTTTTTAGCGTGTTTCTCTTACGCCGCTTTTGCC
GCGACTTCCGTCTCCGGACG

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKYIGAHVSAAGGLANAPARAAEIGATAFALFTKNQRQWRAAPLTPQVIDDFKIACEKYHFSAAQILPHDSYLINLGHPV
SEALEKSRVAFLDEMQRCEQLGLTLLNFHPGSHLMQIAQEDCLARIAESINIALAQTEGVTAVIENTAGQGSNLGFEFEQ
LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPEACEKTFAGFGKIVGFQYLRGMHLNDAKSAFGSRVDRHHSLGEGNIG
HDAFRWIMQDARFDGIPLILETINPDIWAEEIAWLKAQQIAEAVA

Sequences:

>Translated_285_residues
MKYIGAHVSAAGGLANAPARAAEIGATAFALFTKNQRQWRAAPLTPQVIDDFKIACEKYHFSAAQILPHDSYLINLGHPV
SEALEKSRVAFLDEMQRCEQLGLTLLNFHPGSHLMQIAQEDCLARIAESINIALAQTEGVTAVIENTAGQGSNLGFEFEQ
LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPEACEKTFAGFGKIVGFQYLRGMHLNDAKSAFGSRVDRHHSLGEGNIG
HDAFRWIMQDARFDGIPLILETINPDIWAEEIAWLKAQQIAEAVA
>Mature_285_residues
MKYIGAHVSAAGGLANAPARAAEIGATAFALFTKNQRQWRAAPLTPQVIDDFKIACEKYHFSAAQILPHDSYLINLGHPV
SEALEKSRVAFLDEMQRCEQLGLTLLNFHPGSHLMQIAQEDCLARIAESINIALAQTEGVTAVIENTAGQGSNLGFEFEQ
LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPEACEKTFAGFGKIVGFQYLRGMHLNDAKSAFGSRVDRHHSLGEGNIG
HDAFRWIMQDARFDGIPLILETINPDIWAEEIAWLKAQQIAEAVA

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=285, Percent_Identity=88.4210526315789, Blast_Score=531, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI17531193, Length=261, Percent_Identity=50.1915708812261, Blast_Score=288, Evalue=3e-78,
Organism=Saccharomyces cerevisiae, GI6322735, Length=285, Percent_Identity=42.1052631578947, Blast_Score=238, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 31091; Mature: 31091

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIGAHVSAAGGLANAPARAAEIGATAFALFTKNQRQWRAAPLTPQVIDDFKIACEKYH
CCCCCCCHHHCCCCCCCCCHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
FSAAQILPHDSYLINLGHPVSEALEKSRVAFLDEMQRCEQLGLTLLNFHPGSHLMQIAQE
CHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHH
DCLARIAESINIALAQTEGVTAVIENTAGQGSNLGFEFEQLAAIIDGVEDKSRVGVCIDT
HHHHHHHHHHHEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHH
CHAFAAGYDLRTPEACEKTFAGFGKIVGFQYLRGMHLNDAKSAFGSRVDRHHSLGEGNIG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCC
HDAFRWIMQDARFDGIPLILETINPDIWAEEIAWLKAQQIAEAVA
HHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYIGAHVSAAGGLANAPARAAEIGATAFALFTKNQRQWRAAPLTPQVIDDFKIACEKYH
CCCCCCCHHHCCCCCCCCCHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
FSAAQILPHDSYLINLGHPVSEALEKSRVAFLDEMQRCEQLGLTLLNFHPGSHLMQIAQE
CHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHH
DCLARIAESINIALAQTEGVTAVIENTAGQGSNLGFEFEQLAAIIDGVEDKSRVGVCIDT
HHHHHHHHHHHEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHH
CHAFAAGYDLRTPEACEKTFAGFGKIVGFQYLRGMHLNDAKSAFGSRVDRHHSLGEGNIG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCC
HDAFRWIMQDARFDGIPLILETINPDIWAEEIAWLKAQQIAEAVA
HHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA