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The map label for this gene is amiD [H]

Identifier: 194736394

GI number: 194736394

Start: 1016089

End: 1016919

Strand: Direct

Name: amiD [H]

Synonym: SeSA_A1048

Alternate gene names: 194736394

Gene position: 1016089-1016919 (Clockwise)

Preceding gene: 194737439

Following gene: 194735452

Centisome position: 21.58

GC content: 57.4

Gene sequence:

>831_bases
ATGAGAGCGCTACTGTGGCTGGTGGGTCTCGCGTTGCTGTTAACAGGCTGCGCGAGCGAAAAAGGAATTATCGATAAAGA
GGGATATCAGCTTGATACCCGACATCGGGCGCAGGCGGCCTATCCGCGCATTAAAGTTCTGGTGATTCACTATACGGCGG
AAAACTTTGACGTTTCGCTGGCGACGTTAACGGGTCGCAACGTCAGTTCGCATTACCTGATTCCCGCAACCCCTCCATTA
TATGGCGGTAAACCGCGCATCTGGCAACTGGTGCCGGAACAGGATCAGGCCTGGCATGCGGGCGTCAGTTTCTGGCGAGG
CGCCACGCGTCTCAATGATACGTCTATTGGCATTGAGCTGGAAAATCGCGGCTGGCGAATGTCCGGCGGGGTGAAATCTT
TCGCGCCGTTTGAATCCGCGCAAATTCAGGCATTGATTCCGTTAGCGAAGGACATTATCGCGCGCTATGACATCAAACCG
CAGAATGTGGTGGCCCATGCGGATATCGCGCCGCAGCGTAAAGACGATCCCGGCCCGCGCTTCCCGTGGCGCGAGCTGGC
GGCGCAGGGGATTGGCGCCTGGCCTGACGCCCAGCGTGTGGCGTTTTATCTGGCTGGACGCGCGCCGTATACGCCAGTCG
ATACCGCAACGGTGCTTGCGTTACTCTCGCGCTATGGTTATGAAGTCAAAGCCGATATGACGGCACGCGAGCAGCAGCGG
GTGATTATGGCGTTCCAGATGCACTTCCGTCCGGCGCAATGGAACGGTATCGCAGATGCCGAAACGCAGGCGATTGCCGA
AGCATTACTGGAGAAGTACGGCCAGGATTAA

Upstream 100 bases:

>100_bases
CCGATGCGGTGGTAGGCATCGATATTGATTACGAAACTGTCGGCAAGGATGGCAGTATGCTGATGGTCAGCGTCAGCGGA
ACGGCGGTGAAAACGCGCCG

Downstream 100 bases:

>100_bases
CGCGGTAAATTTCCGTGATCGCGTAGCCAGGCGGCGGTGCGTTCAATGCCTTCGTCCAGCGTGACGATGGGCTGGTAGCC
CAGCTCTTCCTGCGCGCGAG

Product: N-acetylmuramoyl-L-alanine amidase AmiD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MRALLWLVGLALLLTGCASEKGIIDKEGYQLDTRHRAQAAYPRIKVLVIHYTAENFDVSLATLTGRNVSSHYLIPATPPL
YGGKPRIWQLVPEQDQAWHAGVSFWRGATRLNDTSIGIELENRGWRMSGGVKSFAPFESAQIQALIPLAKDIIARYDIKP
QNVVAHADIAPQRKDDPGPRFPWRELAAQGIGAWPDAQRVAFYLAGRAPYTPVDTATVLALLSRYGYEVKADMTAREQQR
VIMAFQMHFRPAQWNGIADAETQAIAEALLEKYGQD

Sequences:

>Translated_276_residues
MRALLWLVGLALLLTGCASEKGIIDKEGYQLDTRHRAQAAYPRIKVLVIHYTAENFDVSLATLTGRNVSSHYLIPATPPL
YGGKPRIWQLVPEQDQAWHAGVSFWRGATRLNDTSIGIELENRGWRMSGGVKSFAPFESAQIQALIPLAKDIIARYDIKP
QNVVAHADIAPQRKDDPGPRFPWRELAAQGIGAWPDAQRVAFYLAGRAPYTPVDTATVLALLSRYGYEVKADMTAREQQR
VIMAFQMHFRPAQWNGIADAETQAIAEALLEKYGQD
>Mature_276_residues
MRALLWLVGLALLLTGCASEKGIIDKEGYQLDTRHRAQAAYPRIKVLVIHYTAENFDVSLATLTGRNVSSHYLIPATPPL
YGGKPRIWQLVPEQDQAWHAGVSFWRGATRLNDTSIGIELENRGWRMSGGVKSFAPFESAQIQALIPLAKDIIARYDIKP
QNVVAHADIAPQRKDDPGPRFPWRELAAQGIGAWPDAQRVAFYLAGRAPYTPVDTATVLALLSRYGYEVKADMTAREQQR
VIMAFQMHFRPAQWNGIADAETQAIAEALLEKYGQD

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=276, Percent_Identity=81.8840579710145, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1786300, Length=97, Percent_Identity=45.360824742268, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 30766; Mature: 30766

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALLWLVGLALLLTGCASEKGIIDKEGYQLDTRHRAQAAYPRIKVLVIHYTAENFDVSL
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCHHHHHHCCCEEEEEEEEEECCCCEEEE
ATLTGRNVSSHYLIPATPPLYGGKPRIWQLVPEQDQAWHAGVSFWRGATRLNDTSIGIEL
EEEECCCCCCCEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEE
ENRGWRMSGGVKSFAPFESAQIQALIPLAKDIIARYDIKPQNVVAHADIAPQRKDDPGPR
CCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCC
FPWRELAAQGIGAWPDAQRVAFYLAGRAPYTPVDTATVLALLSRYGYEVKADMTAREQQR
CCHHHHHHCCCCCCCCHHEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHH
VIMAFQMHFRPAQWNGIADAETQAIAEALLEKYGQD
HHEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRALLWLVGLALLLTGCASEKGIIDKEGYQLDTRHRAQAAYPRIKVLVIHYTAENFDVSL
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCHHHHHHCCCEEEEEEEEEECCCCEEEE
ATLTGRNVSSHYLIPATPPLYGGKPRIWQLVPEQDQAWHAGVSFWRGATRLNDTSIGIEL
EEEECCCCCCCEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEE
ENRGWRMSGGVKSFAPFESAQIQALIPLAKDIIARYDIKPQNVVAHADIAPQRKDDPGPR
CCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCC
FPWRELAAQGIGAWPDAQRVAFYLAGRAPYTPVDTATVLALLSRYGYEVKADMTAREQQR
CCHHHHHHCCCCCCCCHHEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHH
VIMAFQMHFRPAQWNGIADAETQAIAEALLEKYGQD
HHEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]