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The map label for this gene is nuoE [H]

Identifier: 194736216

GI number: 194736216

Start: 2439084

End: 2439584

Strand: Reverse

Name: nuoE [H]

Synonym: SeSA_A2553

Alternate gene names: 194736216

Gene position: 2439584-2439084 (Counterclockwise)

Preceding gene: 194736818

Following gene: 194736167

Centisome position: 51.81

GC content: 56.49

Gene sequence:

>501_bases
ATGCACGAGAATCAACAACCACAAACCGAGGCTTTTGAGCTGAGTGCGGCAGAGCGTGAAGCGATAGAGCACGAGAAGCA
CCACTACGAAGACCCGCGTGCGGCGTCCATTGAAGCGCTGAAAATCGTTCAGAAGCAGCGTGGCTGGGTGCCGGACGGCG
CGATCTACGCTATTGCCGATGTGCTGGGTATTCCGGCGAGCGACGTCGAAGGCGTCGCCACATTCTACAGCCAGATCTTC
CGTCAGCCGGTCGGTCGCCACGTGATTCGCTACTGCGACAGCGTGGTGTGCCATATCACCGGCTATCAGGGGATTCAGGC
CGCGCTGGAAAAAAATCTCAACATCAAACCGGGGCAGACGACCTTTGATGGTCGCTTTACGCTGCTGCCGACCTGCTGCC
TGGGGAATTGCGATAAAGGGCCGAACATGATGATTGATGAGGATACTCACAGCCATCTGACCCCGGAAGCCATTCCTGAA
CTACTGGAGCGGTATAAATGA

Upstream 100 bases:

>100_bases
TGCAGCAGATTCCCTCGGCGATCCGCGGTAGCCTGGTCTCCGACCTGATTGTCTATCTGGGTAGTATCGATTTTGTTATG
TCAGATGTGGACCGCTAATT

Downstream 100 bases:

>100_bases
AAAACGTTATCCGTACTCCCGAAACCCATCCGCTGACCTGGCGTCTGCGCGATGACAAACAGCCGGTCTGGCTGGATGAA
TATCGTAGCAAAAACGGCTA

Product: NADH dehydrogenase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E; NUO5 [H]

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MHENQQPQTEAFELSAAEREAIEHEKHHYEDPRAASIEALKIVQKQRGWVPDGAIYAIADVLGIPASDVEGVATFYSQIF
RQPVGRHVIRYCDSVVCHITGYQGIQAALEKNLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHSHLTPEAIPE
LLERYK

Sequences:

>Translated_166_residues
MHENQQPQTEAFELSAAEREAIEHEKHHYEDPRAASIEALKIVQKQRGWVPDGAIYAIADVLGIPASDVEGVATFYSQIF
RQPVGRHVIRYCDSVVCHITGYQGIQAALEKNLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHSHLTPEAIPE
LLERYK
>Mature_166_residues
MHENQQPQTEAFELSAAEREAIEHEKHHYEDPRAASIEALKIVQKQRGWVPDGAIYAIADVLGIPASDVEGVATFYSQIF
RQPVGRHVIRYCDSVVCHITGYQGIQAALEKNLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHSHLTPEAIPE
LLERYK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=157, Percent_Identity=34.3949044585987, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1788621, Length=166, Percent_Identity=96.9879518072289, Blast_Score=340, Evalue=4e-95,
Organism=Caenorhabditis elegans, GI17561328, Length=157, Percent_Identity=35.031847133758, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI18859877, Length=157, Percent_Identity=33.1210191082803, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21355353, Length=130, Percent_Identity=32.3076923076923, Blast_Score=87, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18602; Mature: 18602

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHENQQPQTEAFELSAAEREAIEHEKHHYEDPRAASIEALKIVQKQRGWVPDGAIYAIAD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
VLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHITGYQGIQAALEKNLNIKPGQT
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCC
TFDGRFTLLPTCCLGNCDKGPNMMIDEDTHSHLTPEAIPELLERYK
CCCCCEEEEHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MHENQQPQTEAFELSAAEREAIEHEKHHYEDPRAASIEALKIVQKQRGWVPDGAIYAIAD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
VLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHITGYQGIQAALEKNLNIKPGQT
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCC
TFDGRFTLLPTCCLGNCDKGPNMMIDEDTHSHLTPEAIPELLERYK
CCCCCEEEEHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]