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The map label for this gene is ascG [H]

Identifier: 194736198

GI number: 194736198

Start: 3075942

End: 3076961

Strand: Direct

Name: ascG [H]

Synonym: SeSA_A3180

Alternate gene names: 194736198

Gene position: 3075942-3076961 (Clockwise)

Preceding gene: 194736933

Following gene: 194737023

Centisome position: 65.32

GC content: 57.35

Gene sequence:

>1020_bases
ATGGCGACAATGCTGGATGTTTCCCGCCATGCGGGCGTATCAAAGGCCACCGTCTCACGAGTGCTGAATGGGACGGGGCA
GGTAAAAGAAAGCACGCGCCAGAAAGTGTTTACGGCGATGCAGGCTCTGGACTATCGCCCCAACCTGCTGGCACGCTCGC
TGGCGAATCGCACCAGCAACAGCATCGGTCTGGTCGTCTCTACGTTTGACGGCTTCTATTTTGGCAGTTTGTTGCGCCGG
GCGTCGCGCCAGGCGGAGTTTCATAACAAGCAGTTGATCGTCACCGATGGTCACGATACGCCGGAACGAGAGCAGAAAGC
CGTACAAATGTTGGCCGACAGACAGTGCGACGCTATTATTCTTTACACTCGCTATATGGATGAGCCGGCGATTTTGTCGT
TGATTGACGCCACGGAAATGCCGCTGGTGATTATTAATCGCAACGTCACTCAGGCCCGCGATCGCGCTATTTTCTTCGAG
CAGGAGACGGCGGCATTCCAGGCGGTGGAATATCTGATTACGCAGGGCCATCGCGATATCGCCTGTATTACGCTGCCTGT
TCATACTCCCACCGGCACATCACGCGTAGCGGGTTATCGCAAGGCGCTGGAAAAATATGGCATTCCCTGGCAACCGGCAA
AAGTGAAATACGGCGATTACACGCTGACGCGCGGCTATGACGCCTGCCGGGAATTACTGGAGGAAGGCGTCACGTTTAGC
GCGCTATTCGCCTGTAATGATGACACGGCGCTGGGCGCGGCAAAAGCGCTGCGCCAGGCCGGATTACGCATCCCGCAGGA
TGTGTCGCTGTTTGGTTTTGACGATGCGCCGGGCGCAACCTGGCTTGAACCGGGGCTTTCAACAGTCTATTTACCCATCG
AGGATATGATAGCCACCGCAATCGATCAGGCCGTTCGTCTGGCGAACAGCGAGCCGGTCGCCCCGATCCCGCCCTTTACC
GGCACGCTGATTCTGCGCGAGTCCGTCGCCGCAGGCCCGTTTTTTCAACGTCCGGCCTAA

Upstream 100 bases:

>100_bases
GTAACTCATGTATTTAGTCCGACACTGGTACGCCGACATTCGGTATCCACGCCGACGGATACCGGACACCTGTCGACAAC
CGATTAATCAGGGTAGCATG

Downstream 100 bases:

>100_bases
TGATGCGCTTCAGAGCATCTCCAGCGCTAACAGCTCTTCAATGGTCTGACGACGGCGGATCAGCCGCGCCTGTCCATTGT
CAAACAGCACTTCCGGCAGG

Product: HTH-type transcriptional regulator AscG

Products: NA

Alternate protein names: Cryptic asc operon repressor [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MATMLDVSRHAGVSKATVSRVLNGTGQVKESTRQKVFTAMQALDYRPNLLARSLANRTSNSIGLVVSTFDGFYFGSLLRR
ASRQAEFHNKQLIVTDGHDTPEREQKAVQMLADRQCDAIILYTRYMDEPAILSLIDATEMPLVIINRNVTQARDRAIFFE
QETAAFQAVEYLITQGHRDIACITLPVHTPTGTSRVAGYRKALEKYGIPWQPAKVKYGDYTLTRGYDACRELLEEGVTFS
ALFACNDDTALGAAKALRQAGLRIPQDVSLFGFDDAPGATWLEPGLSTVYLPIEDMIATAIDQAVRLANSEPVAPIPPFT
GTLILRESVAAGPFFQRPA

Sequences:

>Translated_339_residues
MATMLDVSRHAGVSKATVSRVLNGTGQVKESTRQKVFTAMQALDYRPNLLARSLANRTSNSIGLVVSTFDGFYFGSLLRR
ASRQAEFHNKQLIVTDGHDTPEREQKAVQMLADRQCDAIILYTRYMDEPAILSLIDATEMPLVIINRNVTQARDRAIFFE
QETAAFQAVEYLITQGHRDIACITLPVHTPTGTSRVAGYRKALEKYGIPWQPAKVKYGDYTLTRGYDACRELLEEGVTFS
ALFACNDDTALGAAKALRQAGLRIPQDVSLFGFDDAPGATWLEPGLSTVYLPIEDMIATAIDQAVRLANSEPVAPIPPFT
GTLILRESVAAGPFFQRPA
>Mature_338_residues
ATMLDVSRHAGVSKATVSRVLNGTGQVKESTRQKVFTAMQALDYRPNLLARSLANRTSNSIGLVVSTFDGFYFGSLLRRA
SRQAEFHNKQLIVTDGHDTPEREQKAVQMLADRQCDAIILYTRYMDEPAILSLIDATEMPLVIINRNVTQARDRAIFFEQ
ETAAFQAVEYLITQGHRDIACITLPVHTPTGTSRVAGYRKALEKYGIPWQPAKVKYGDYTLTRGYDACRELLEEGVTFSA
LFACNDDTALGAAKALRQAGLRIPQDVSLFGFDDAPGATWLEPGLSTVYLPIEDMIATAIDQAVRLANSEPVAPIPPFTG
TLILRESVAAGPFFQRPA

Specific function: Repressor of the asc operon. The cryptic operon is activated by the insertion of IS186 into the ascG gene [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=333, Percent_Identity=40.5405405405405, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI1788474, Length=333, Percent_Identity=34.5345345345345, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI1789202, Length=333, Percent_Identity=34.5345345345345, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=31.8452380952381, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1790194, Length=302, Percent_Identity=31.1258278145695, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1790369, Length=292, Percent_Identity=30.4794520547945, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1786540, Length=330, Percent_Identity=28.7878787878788, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787580, Length=317, Percent_Identity=26.813880126183, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI48994940, Length=311, Percent_Identity=28.6173633440514, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1787906, Length=283, Percent_Identity=26.5017667844523, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790715, Length=293, Percent_Identity=23.8907849829352, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786268, Length=156, Percent_Identity=30.7692307692308, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790192, Length=306, Percent_Identity=26.1437908496732, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790689, Length=293, Percent_Identity=24.2320819112628, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37321; Mature: 37190

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMLDVSRHAGVSKATVSRVLNGTGQVKESTRQKVFTAMQALDYRPNLLARSLANRTSN
CCCHHCCHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
SIGLVVSTFDGFYFGSLLRRASRQAEFHNKQLIVTDGHDTPEREQKAVQMLADRQCDAII
CEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEE
LYTRYMDEPAILSLIDATEMPLVIINRNVTQARDRAIFFEQETAAFQAVEYLITQGHRDI
EEEECCCCCHHHHHHCCCCCCEEEEECCCHHHHCCEEEEEHHHHHHHHHHHHHHCCCCCE
ACITLPVHTPTGTSRVAGYRKALEKYGIPWQPAKVKYGDYTLTRGYDACRELLEEGVTFS
EEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEECCEEEECCHHHHHHHHHCCCCEE
ALFACNDDTALGAAKALRQAGLRIPQDVSLFGFDDAPGATWLEPGLSTVYLPIEDMIATA
EEEEECCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEECCCCCEEEECHHHHHHHH
IDQAVRLANSEPVAPIPPFTGTLILRESVAAGPFFQRPA
HHHHHHHCCCCCCCCCCCCCCCEEEEHHHHCCCCCCCCC
>Mature Secondary Structure 
ATMLDVSRHAGVSKATVSRVLNGTGQVKESTRQKVFTAMQALDYRPNLLARSLANRTSN
CCHHCCHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
SIGLVVSTFDGFYFGSLLRRASRQAEFHNKQLIVTDGHDTPEREQKAVQMLADRQCDAII
CEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEE
LYTRYMDEPAILSLIDATEMPLVIINRNVTQARDRAIFFEQETAAFQAVEYLITQGHRDI
EEEECCCCCHHHHHHCCCCCCEEEEECCCHHHHCCEEEEEHHHHHHHHHHHHHHCCCCCE
ACITLPVHTPTGTSRVAGYRKALEKYGIPWQPAKVKYGDYTLTRGYDACRELLEEGVTFS
EEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEECCEEEECCHHHHHHHHHCCCCEE
ALFACNDDTALGAAKALRQAGLRIPQDVSLFGFDDAPGATWLEPGLSTVYLPIEDMIATA
EEEEECCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEECCCCCEEEECHHHHHHHH
IDQAVRLANSEPVAPIPPFTGTLILRESVAAGPFFQRPA
HHHHHHHCCCCCCCCCCCCCCCEEEEHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]