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The map label for this gene is rhtB [H]

Identifier: 194736002

GI number: 194736002

Start: 4043519

End: 4044139

Strand: Reverse

Name: rhtB [H]

Synonym: SeSA_A4168

Alternate gene names: 194736002

Gene position: 4044139-4043519 (Counterclockwise)

Preceding gene: 194737515

Following gene: 194736400

Centisome position: 85.88

GC content: 54.91

Gene sequence:

>621_bases
ATGACCTTTGAATGGTGGTTCGCCTATCTACTGACCTCAACTCTGCTGAGTCTTTCTCCGGGTTCAGGCGCCATCAATAC
CATGACGACGTCTATCAACCATGGATATCGTGGCGCAGCGGCTTCTATCGCCGGACTCCAGACCGGGCTGGGGATACATA
TCGTACTGGTGGGCGTCGGACTGGGTACGCTCTTTTCGCGCTCGCTCCTCGCTTTTGAAATTCTGAAATGGGCTGGCGCG
GCTTATCTTATCTGGCTGGGTATCCAGCAATGGCGCGCCGCAGGCGCTATCGATCTGCATACTCTCGCCCAGACGCAATC
ACGGGGTCGGCTCTTCAAACGGGCGATATTTGTCAATCTAACCAATCCCAAAAGCATTGTCTTTCTTGCCGCCCTGTTTC
CGCAATTCATCATGCCGCAGCAACCGCAACTGGCGCAGTACCTCATTCTCGGCGTCACCACGATTGTGGTGGATATGATT
GTGATGACCGGTTACGCCACGCTGGCGCAGCGCATTGCCGCCTGGATTAAAGGACCAAAGCAGATGAAGGCGCTGAATAA
AGCGTTTGGTTCGTTGTTTATGCTGGTAGGCGCGCTCCTGGCGTTGGCAAGACACGCGTGA

Upstream 100 bases:

>100_bases
GCCTCGTTTACCAGGTAAAATCGTTTTTTGCGTCGTGCCTGACACAACACAGCGGCAGTAGCGTATTGTGGCACAAAAAC
AGACAACCAGGGAGTTTCGC

Downstream 100 bases:

>100_bases
TAGTCGTGCCGGATGATGTTTATCCGGCCCGGAGGTAAATTAACGCGAAATAATCAGGTGAATACCAAAGCCCGCAAACA
GAGCGCCGGCAAAGCCATCA

Product: homoserine/homoserine lactone efflux protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MTFEWWFAYLLTSTLLSLSPGSGAINTMTTSINHGYRGAAASIAGLQTGLGIHIVLVGVGLGTLFSRSLLAFEILKWAGA
AYLIWLGIQQWRAAGAIDLHTLAQTQSRGRLFKRAIFVNLTNPKSIVFLAALFPQFIMPQQPQLAQYLILGVTTIVVDMI
VMTGYATLAQRIAAWIKGPKQMKALNKAFGSLFMLVGALLALARHA

Sequences:

>Translated_206_residues
MTFEWWFAYLLTSTLLSLSPGSGAINTMTTSINHGYRGAAASIAGLQTGLGIHIVLVGVGLGTLFSRSLLAFEILKWAGA
AYLIWLGIQQWRAAGAIDLHTLAQTQSRGRLFKRAIFVNLTNPKSIVFLAALFPQFIMPQQPQLAQYLILGVTTIVVDMI
VMTGYATLAQRIAAWIKGPKQMKALNKAFGSLFMLVGALLALARHA
>Mature_205_residues
TFEWWFAYLLTSTLLSLSPGSGAINTMTTSINHGYRGAAASIAGLQTGLGIHIVLVGVGLGTLFSRSLLAFEILKWAGAA
YLIWLGIQQWRAAGAIDLHTLAQTQSRGRLFKRAIFVNLTNPKSIVFLAALFPQFIMPQQPQLAQYLILGVTTIVVDMIV
MTGYATLAQRIAAWIKGPKQMKALNKAFGSLFMLVGALLALARHA

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=206, Percent_Identity=88.3495145631068, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1788099, Length=201, Percent_Identity=26.865671641791, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22335; Mature: 22204

Theoretical pI: Translated: 11.20; Mature: 11.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFEWWFAYLLTSTLLSLSPGSGAINTMTTSINHGYRGAAASIAGLQTGLGIHIVLVGVG
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
LGTLFSRSLLAFEILKWAGAAYLIWLGIQQWRAAGAIDLHTLAQTQSRGRLFKRAIFVNL
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEE
TNPKSIVFLAALFPQFIMPQQPQLAQYLILGVTTIVVDMIVMTGYATLAQRIAAWIKGPK
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
QMKALNKAFGSLFMLVGALLALARHA
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TFEWWFAYLLTSTLLSLSPGSGAINTMTTSINHGYRGAAASIAGLQTGLGIHIVLVGVG
CHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
LGTLFSRSLLAFEILKWAGAAYLIWLGIQQWRAAGAIDLHTLAQTQSRGRLFKRAIFVNL
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEE
TNPKSIVFLAALFPQFIMPQQPQLAQYLILGVTTIVVDMIVMTGYATLAQRIAAWIKGPK
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
QMKALNKAFGSLFMLVGALLALARHA
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]