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The map label for this gene is raiA [H]

Identifier: 194735943

GI number: 194735943

Start: 2760332

End: 2760670

Strand: Direct

Name: raiA [H]

Synonym: SeSA_A2860

Alternate gene names: 194735943

Gene position: 2760332-2760670 (Clockwise)

Preceding gene: 194736160

Following gene: 194735195

Centisome position: 58.62

GC content: 46.9

Gene sequence:

>339_bases
ATGACAATGAACATTACCAGTAAACAAATGGAAATTACTCCGGCAATCCGCCAACATGTCGCAGACCGTCTCGCCAAACT
GGAAAAATGGCAAACTCACCTGATTAATCCTCATATCATTCTGTCTAAAGAGCCACAGGGCTTTATTGCTGACGCCACCA
TTAATACACCGAACGGACATCTGGTCGCCAGCGCAAAACACGAAGATATGTATACCGCTATTAATGAATTGATCAACAAG
CTGGAACGGCAGCTCAATAAAGTGCAGCACAAAGGCGAAGCCCGTCGTGCCACCGCATCGGTGAAAGATGCCAGCTTCGT
CGAAGCAGAAGAAGAGTAG

Upstream 100 bases:

>100_bases
TGACAAAAAGTGACAAAATAATGTGATTTACATCACACATTTTGACATTAAGAACGGTATGCTGGAATCACCAAGACGGG
AAAGACAAGAGGTAAAATTT

Downstream 100 bases:

>100_bases
TCCCTTACATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGGATTAAAACAGTACGAGTACTGTAC
TTACGTACACAAAGGAAACA

Product: translation inhibitor protein RaiA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 112; Mature: 111

Protein sequence:

>112_residues
MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKEPQGFIADATINTPNGHLVASAKHEDMYTAINELINK
LERQLNKVQHKGEARRATASVKDASFVEAEEE

Sequences:

>Translated_112_residues
MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKEPQGFIADATINTPNGHLVASAKHEDMYTAINELINK
LERQLNKVQHKGEARRATASVKDASFVEAEEE
>Mature_111_residues
TMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKEPQGFIADATINTPNGHLVASAKHEDMYTAINELINKL
ERQLNKVQHKGEARRATASVKDASFVEAEEE

Specific function: During environmental stress such as cold shock or excessive cell density at stationary phase, stabilizes the 70S ribosome against dissociation, inhibits translation initiation and increase translation accuracy. When normal growth conditions are restored,

COG id: COG1544

COG function: function code J; Ribosome-associated protein Y (PSrp-1)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma(54) modulation protein family [H]

Homologues:

Organism=Escherichia coli, GI1788949, Length=112, Percent_Identity=91.9642857142857, Blast_Score=214, Evalue=1e-57,
Organism=Escherichia coli, GI1789595, Length=87, Percent_Identity=41.3793103448276, Blast_Score=71, Evalue=2e-14,

Paralogues:

None

Copy number: 600 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003489 [H]

Pfam domain/function: PF02482 Ribosomal_S30AE [H]

EC number: NA

Molecular weight: Translated: 12653; Mature: 12521

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKEPQGFIADATINTPNGH
CCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEECCCCC
LVASAKHEDMYTAINELINKLERQLNKVQHKGEARRATASVKDASFVEAEEE
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKEPQGFIADATINTPNGH
CCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEECCCCC
LVASAKHEDMYTAINELINKLERQLNKVQHKGEARRATASVKDASFVEAEEE
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]