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The map label for this gene is proS [H]

Identifier: 194735941

GI number: 194735941

Start: 284886

End: 286604

Strand: Reverse

Name: proS [H]

Synonym: SeSA_A0269

Alternate gene names: 194735941

Gene position: 286604-284886 (Counterclockwise)

Preceding gene: 194734251

Following gene: 194737176

Centisome position: 6.09

GC content: 54.68

Gene sequence:

>1719_bases
ATGCGTACTAGCCAATACCTGCTCTCCACTCTGAAGGAGACACCTGCCGACGCCGAGGTTATCAGCCACCAGCTGATGCT
GCGCGCCGGGATGATCCGCAAGCTCGCCTCCGGGTTATATACCTGGCTGCCGACCGGCCTGCGCGTCCTGAAAAAAGTCG
AAAACATCGTGCGTGAAGAGATGAACAACGCCGGTGCTATCGAGGTGTCGATGCCAGTCGTTCAGCCAGCCGACCTGTGG
CAAGAGAGTGGTCGTTGGGAACAGTACGGTCCGGAACTGCTGCGTTTTGTCGACCGTGGCGAGCGTCCGTTCGTACTCGG
CCCGACCCACGAAGAAGTTATCACCGATCTGGTGCGTAATGAACTCAGCTCTTATAAGCAGTTGCCGCTGAACTTCTTCC
AAATCCAGACCAAATTCCGTGATGAAGTGCGTCCGCGCTTTGGCGTGATGCGTTCGCGCGAATTCCTGATGAAAGATGCT
TACTCTTTCCACACCTCTCAGGAGTCGTTGCAGGAAACCTATGACGCCATGTACGCCGCGTATAGCCGTATCTTCAGCCG
TATGGGGCTGGATTTCCGCGCGGTACAGGCCGATACCGGTTCTATCGGCGGCAACGCCTCGCATGAATTCCAGGTGCTGG
CGCAAAGCGGCGAAGACGATATCGTTTTCTCTGATGTTTCTGATTACGCGGCTAACATTGAACTGGCAGAAGCTATCGCG
CCGCAAACGCCGCGCGCAGCGGCGACGCAGGAAATGACTCTGGTCGATACGCCAAATGCCAAAACCATCGCGGAGCTGGT
GGAACAGTTCAACCTGCCAATTGAAAAAACGGTAAAAACGCTGCTGGTGAAAGCGGCGAAAGACAGCAAGTCTCCGCTGG
TGGCTCTGCTGGTTCGCGGCGATCATGAACTGAATGAAGTTAAAGCGGAAAAACTGCCGCACGTTGCCAGCCCGCTGACC
TTCGCTACCGAAGAAGAGATTCGCGCCGTAATTAATGCCGGCCCTGGCTCCCTCGGCCCGGTCAATATGTCGATTCCGGT
CATCATCGATCGTACCGTTGCCGCCATGAGCGATTTTGCCGCTGGCGCCAACATCGACGGTAAACACTACTTTGGTATTA
ACTGGGATCGCGATGTGGCGACGCCTGTTGTTGCCGACATCCGTAACGTAGTCGCCGGCGATCCGAGTCCGGATGGTCAG
GGAACGTTGCTTATCAAACGCGGTATCGAAGTGGGTCACATCTTCCAGTTAGGTACCAAGTACTCAGAAGCGCTGAAAGC
CTCCGTTCAGGGTGAAGATGGCCGTAACCAGATCCTGACAATGGGCTGCTATGGTATCGGCGTAACACGTGTCGTTGCCG
CGGCTATCGAGCAGAACTTTGACGAGCGCGGCATTGTATGGCCTGACGCCATTGCGCCTTTCCAGGTCGCTATTCTGCCG
ATGAATATGCACAAATCCTTCCGTGTGCAGGAGCTGGCGGAAAAGCTGTACAGCGAATTGCGCGCGCAGGGCATTGAAGT
GCTGATGGACGACCGTAAAGAGCGCCCGGGCGTGATGTTTGCCGATATGGAGCTGATTGGTATTCCACATACTATCGTGA
TTGGCGATCGCAACCTGGATAACGACGATATTGAGTATAAATACCGTCGTAGCGGTGAAAAATCGCTGATTAAAACCGGC
GATATCGTTGATTATCTGGTCAAGGCGATTAAAGGCTAA

Upstream 100 bases:

>100_bases
TTCTCTTTTGACATCTCTTGCACGCTGGTAAACTAAATCACTTTTTTTGTATCAGGCTCACATTGAGCCTGTTCGATCGT
TCAACTGGAACCGTAACAAC

Downstream 100 bases:

>100_bases
ACGACATTTTGTTGGGATTGCCCGGCGCCACCCGGCAGATATGTAAAGGCCTCGCATCCTGCGAGGCCTTTTATTAGTGA
GTGCAATCTTTACCCGGCGC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGLRVLKKVENIVREEMNNAGAIEVSMPVVQPADLW
QESGRWEQYGPELLRFVDRGERPFVLGPTHEEVITDLVRNELSSYKQLPLNFFQIQTKFRDEVRPRFGVMRSREFLMKDA
YSFHTSQESLQETYDAMYAAYSRIFSRMGLDFRAVQADTGSIGGNASHEFQVLAQSGEDDIVFSDVSDYAANIELAEAIA
PQTPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEKTVKTLLVKAAKDSKSPLVALLVRGDHELNEVKAEKLPHVASPLT
FATEEEIRAVINAGPGSLGPVNMSIPVIIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPVVADIRNVVAGDPSPDGQ
GTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTRVVAAAIEQNFDERGIVWPDAIAPFQVAILP
MNMHKSFRVQELAEKLYSELRAQGIEVLMDDRKERPGVMFADMELIGIPHTIVIGDRNLDNDDIEYKYRRSGEKSLIKTG
DIVDYLVKAIKG

Sequences:

>Translated_572_residues
MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGLRVLKKVENIVREEMNNAGAIEVSMPVVQPADLW
QESGRWEQYGPELLRFVDRGERPFVLGPTHEEVITDLVRNELSSYKQLPLNFFQIQTKFRDEVRPRFGVMRSREFLMKDA
YSFHTSQESLQETYDAMYAAYSRIFSRMGLDFRAVQADTGSIGGNASHEFQVLAQSGEDDIVFSDVSDYAANIELAEAIA
PQTPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEKTVKTLLVKAAKDSKSPLVALLVRGDHELNEVKAEKLPHVASPLT
FATEEEIRAVINAGPGSLGPVNMSIPVIIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPVVADIRNVVAGDPSPDGQ
GTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTRVVAAAIEQNFDERGIVWPDAIAPFQVAILP
MNMHKSFRVQELAEKLYSELRAQGIEVLMDDRKERPGVMFADMELIGIPHTIVIGDRNLDNDDIEYKYRRSGEKSLIKTG
DIVDYLVKAIKG
>Mature_572_residues
MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGLRVLKKVENIVREEMNNAGAIEVSMPVVQPADLW
QESGRWEQYGPELLRFVDRGERPFVLGPTHEEVITDLVRNELSSYKQLPLNFFQIQTKFRDEVRPRFGVMRSREFLMKDA
YSFHTSQESLQETYDAMYAAYSRIFSRMGLDFRAVQADTGSIGGNASHEFQVLAQSGEDDIVFSDVSDYAANIELAEAIA
PQTPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEKTVKTLLVKAAKDSKSPLVALLVRGDHELNEVKAEKLPHVASPLT
FATEEEIRAVINAGPGSLGPVNMSIPVIIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPVVADIRNVVAGDPSPDGQ
GTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTRVVAAAIEQNFDERGIVWPDAIAPFQVAILP
MNMHKSFRVQELAEKLYSELRAQGIEVLMDDRKERPGVMFADMELIGIPHTIVIGDRNLDNDDIEYKYRRSGEKSLIKTG
DIVDYLVKAIKG

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=44.2477876106195, Blast_Score=214, Evalue=2e-55,
Organism=Escherichia coli, GI1786392, Length=572, Percent_Identity=94.9300699300699, Blast_Score=1125, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=261, Percent_Identity=37.9310344827586, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI193203271, Length=89, Percent_Identity=38.2022471910112, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=545, Percent_Identity=30.2752293577982, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24656200, Length=220, Percent_Identity=38.1818181818182, Blast_Score=179, Evalue=5e-45,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63502; Mature: 63502

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGLRVLKKVENIVREE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGERPFVLGPTHEEVITDLVRN
HCCCCEEEEECCCCCCHHHHHHCCCCHHHCHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
ELSSYKQLPLNFFQIQTKFRDEVRPRFGVMRSREFLMKDAYSFHTSQESLQETYDAMYAA
HHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YSRIFSRMGLDFRAVQADTGSIGGNASHEFQVLAQSGEDDIVFSDVSDYAANIELAEAIA
HHHHHHHHCCCEEEEECCCCCCCCCCCCHHHEEECCCCCCEEEECHHHHHHCCHHHHHHC
PQTPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEKTVKTLLVKAAKDSKSPLVALLVRG
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEEC
DHELNEVKAEKLPHVASPLTFATEEEIRAVINAGPGSLGPVNMSIPVIIDRTVAAMSDFA
CCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHH
AGANIDGKHYFGINWDRDVATPVVADIRNVVAGDPSPDGQGTLLIKRGIEVGHIFQLGTK
CCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHH
YSEALKASVQGEDGRNQILTMGCYGIGVTRVVAAAIEQNFDERGIVWPDAIAPFQVAILP
HHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEE
MNMHKSFRVQELAEKLYSELRAQGIEVLMDDRKERPGVMFADMELIGIPHTIVIGDRNLD
ECCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEECEEEECCCEEEEEECCCCC
NDDIEYKYRRSGEKSLIKTGDIVDYLVKAIKG
CCCCCCHHHCCCCHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGLRVLKKVENIVREE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGERPFVLGPTHEEVITDLVRN
HCCCCEEEEECCCCCCHHHHHHCCCCHHHCHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
ELSSYKQLPLNFFQIQTKFRDEVRPRFGVMRSREFLMKDAYSFHTSQESLQETYDAMYAA
HHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YSRIFSRMGLDFRAVQADTGSIGGNASHEFQVLAQSGEDDIVFSDVSDYAANIELAEAIA
HHHHHHHHCCCEEEEECCCCCCCCCCCCHHHEEECCCCCCEEEECHHHHHHCCHHHHHHC
PQTPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEKTVKTLLVKAAKDSKSPLVALLVRG
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEEC
DHELNEVKAEKLPHVASPLTFATEEEIRAVINAGPGSLGPVNMSIPVIIDRTVAAMSDFA
CCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHH
AGANIDGKHYFGINWDRDVATPVVADIRNVVAGDPSPDGQGTLLIKRGIEVGHIFQLGTK
CCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHH
YSEALKASVQGEDGRNQILTMGCYGIGVTRVVAAAIEQNFDERGIVWPDAIAPFQVAILP
HHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEE
MNMHKSFRVQELAEKLYSELRAQGIEVLMDDRKERPGVMFADMELIGIPHTIVIGDRNLD
ECCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEECEEEECCCEEEEEECCCCC
NDDIEYKYRRSGEKSLIKTGDIVDYLVKAIKG
CCCCCCHHHCCCCHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA