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The map label for this gene is yaaJ [H]

Identifier: 194735924

GI number: 194735924

Start: 5966

End: 7423

Strand: Reverse

Name: yaaJ [H]

Synonym: SeSA_A0006

Alternate gene names: 194735924

Gene position: 7423-5966 (Counterclockwise)

Preceding gene: 194735180

Following gene: 194738139

Centisome position: 0.16

GC content: 55.76

Gene sequence:

>1458_bases
GTGCCGGCATCAACAAGAGGGACGTTAATGCCTGAGTTTTTCAGTTTTATTAACGAAATACTCTGGGGCTCGGTAATGAT
TTACCTGCTGCTCGGCGCAGGATGTTGGTTTACCTGGCGTACCGGATTCATTCAGTTTCGTTATATTCGCCAATTTGGCC
GCAGTCTGAAAGGCAGCCTTAGCCCGCAGCCAAGCGGCCTGACGTCATTTCAGGCGCTGTGTACCAGCCTCGCGGCGCGG
ATTGGCAGCGGTAATCTGGCCGGCGTGGCGCTGGCTATCGCCGCAGGCGGTCCCGGCGCGGTATTCTGGATGTGGGTCTC
GGCCATCATTGGCATGGCGACCTCCTTCGCCGAGTGTTCGCTTGCCCAGCTTTATAAAGAACGTGACCCGACAGGCCAGT
TCCGCGGCGGCCCGGCGTGGTATATGGCGCGCGGGCTGGGAATGCGCTGGATGGGCGTGGTTTTCGCCCTCTTTCTGCTC
GTCGCCTACGGGTTGATTTTTAATAGCGTGCAGGCGAATGCCGTCTCACGCGCGCTCCATTTCGCCTTCAACATTCCGCC
GCTTATCTCCGGCATTGCTCTGGCGTTCTGTGCCCTGTTAATTATCATTCGCGGCATAAAAGGCGTCGCCCGCCTGATGC
AATGGCTGATTCCCCTCATCGCGTTATTGTGGGTCGCCGGCAGCGTATTCATTTGCCTCTGGCATATTGAGCAAATGCCT
GGCGTTATCGCCTCGATAGTCAAAAGCGCATTTGGCTGGCAGGAAGCCGCGGCAGGCGCAGCCGGATATACGCTCACTCA
GGCTATTACCAGCGGTTTTCAGCGTGGGATGTTCTCTAATGAAGCGGGAATGGGGTCAACGCCTAATGCGGCCGCAGCGG
CAACGTCGTATCCCCCTCACCCTGTCGCGCAGGGGATTGTGCAAATGATTGGCGTATTCAGCGACACCATTATCATCTGT
ACCGCCAGCGCGATGATTATCCTGCTGGCGGGGAATCACGCGTCCCACTCGTCAACAGAAGGCATTCAGCTTCTCCAACA
TGCAATGGTTTCGTTGACCGGTGAATGGGGCGCCAGTTTTGTCGCGCTTATCGTCATTCTGTTCGCGTTCAGTTCTATCG
TCGCCAACTATATTTACGCTGAAAACAATCTGTTTTTTCTGCGGTTACATAACGCAAAGGCCATCTGGCTATTACGCCTT
GCGACGCTTGGCATGGTTATCGCCGGTACGCTTATCAGTTTTCCGCTGATATGGCAGCTCGCTGACATGATCATGGCCTG
TATGGCCATTACCAATCTGACGGCGATTTTGTTGCTCTCACCGGTGGTGTATACCCTCGCCAGCGATTATCTGCGACAAC
GGAAACTGGGCGTAAGACCGCAGTTCGATCCGCGGCGCTTCCCGGATATCGAACCACAACTGGCGCCAGATACCTGGGAT
GCGGCATCGCGCGATTAA

Upstream 100 bases:

>100_bases
CAATTAAAGGATACCGCCGTATTTATGGTAAACTGCCGTTAATATTAGTCTGACAAGGTCATCTACATCATGGGTCACGC
TTATACAGGATTATCAAAGC

Downstream 100 bases:

>100_bases
GCCGACAATCGCAACCATCGGTCAACTCTGCGGCGTTTTTTTGCTAAAGTCGCAGAAAATTCCCTGCAAGGACTGGATAT
GCTGATTCTGATTTCACCTG

Product: amino-acid carrier protein AlsT

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MPASTRGTLMPEFFSFINEILWGSVMIYLLLGAGCWFTWRTGFIQFRYIRQFGRSLKGSLSPQPSGLTSFQALCTSLAAR
IGSGNLAGVALAIAAGGPGAVFWMWVSAIIGMATSFAECSLAQLYKERDPTGQFRGGPAWYMARGLGMRWMGVVFALFLL
VAYGLIFNSVQANAVSRALHFAFNIPPLISGIALAFCALLIIIRGIKGVARLMQWLIPLIALLWVAGSVFICLWHIEQMP
GVIASIVKSAFGWQEAAAGAAGYTLTQAITSGFQRGMFSNEAGMGSTPNAAAAATSYPPHPVAQGIVQMIGVFSDTIIIC
TASAMIILLAGNHASHSSTEGIQLLQHAMVSLTGEWGASFVALIVILFAFSSIVANYIYAENNLFFLRLHNAKAIWLLRL
ATLGMVIAGTLISFPLIWQLADMIMACMAITNLTAILLLSPVVYTLASDYLRQRKLGVRPQFDPRRFPDIEPQLAPDTWD
AASRD

Sequences:

>Translated_485_residues
MPASTRGTLMPEFFSFINEILWGSVMIYLLLGAGCWFTWRTGFIQFRYIRQFGRSLKGSLSPQPSGLTSFQALCTSLAAR
IGSGNLAGVALAIAAGGPGAVFWMWVSAIIGMATSFAECSLAQLYKERDPTGQFRGGPAWYMARGLGMRWMGVVFALFLL
VAYGLIFNSVQANAVSRALHFAFNIPPLISGIALAFCALLIIIRGIKGVARLMQWLIPLIALLWVAGSVFICLWHIEQMP
GVIASIVKSAFGWQEAAAGAAGYTLTQAITSGFQRGMFSNEAGMGSTPNAAAAATSYPPHPVAQGIVQMIGVFSDTIIIC
TASAMIILLAGNHASHSSTEGIQLLQHAMVSLTGEWGASFVALIVILFAFSSIVANYIYAENNLFFLRLHNAKAIWLLRL
ATLGMVIAGTLISFPLIWQLADMIMACMAITNLTAILLLSPVVYTLASDYLRQRKLGVRPQFDPRRFPDIEPQLAPDTWD
AASRD
>Mature_484_residues
PASTRGTLMPEFFSFINEILWGSVMIYLLLGAGCWFTWRTGFIQFRYIRQFGRSLKGSLSPQPSGLTSFQALCTSLAARI
GSGNLAGVALAIAAGGPGAVFWMWVSAIIGMATSFAECSLAQLYKERDPTGQFRGGPAWYMARGLGMRWMGVVFALFLLV
AYGLIFNSVQANAVSRALHFAFNIPPLISGIALAFCALLIIIRGIKGVARLMQWLIPLIALLWVAGSVFICLWHIEQMPG
VIASIVKSAFGWQEAAAGAAGYTLTQAITSGFQRGMFSNEAGMGSTPNAAAAATSYPPHPVAQGIVQMIGVFSDTIIICT
ASAMIILLAGNHASHSSTEGIQLLQHAMVSLTGEWGASFVALIVILFAFSSIVANYIYAENNLFFLRLHNAKAIWLLRLA
TLGMVIAGTLISFPLIWQLADMIMACMAITNLTAILLLSPVVYTLASDYLRQRKLGVRPQFDPRRFPDIEPQLAPDTWDA
ASRD

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=476, Percent_Identity=76.4705882352941, Blast_Score=717, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 52439; Mature: 52308

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASTRGTLMPEFFSFINEILWGSVMIYLLLGAGCWFTWRTGFIQFRYIRQFGRSLKGSL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
SPQPSGLTSFQALCTSLAARIGSGNLAGVALAIAAGGPGAVFWMWVSAIIGMATSFAECS
CCCCCCHHHHHHHHHHHHHHHCCCCHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
LAQLYKERDPTGQFRGGPAWYMARGLGMRWMGVVFALFLLVAYGLIFNSVQANAVSRALH
HHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAFNIPPLISGIALAFCALLIIIRGIKGVARLMQWLIPLIALLWVAGSVFICLWHIEQMP
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVIASIVKSAFGWQEAAAGAAGYTLTQAITSGFQRGMFSNEAGMGSTPNAAAAATSYPPH
HHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCC
PVAQGIVQMIGVFSDTIIICTASAMIILLAGNHASHSSTEGIQLLQHAMVSLTGEWGASF
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHH
VALIVILFAFSSIVANYIYAENNLFFLRLHNAKAIWLLRLATLGMVIAGTLISFPLIWQL
HHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADMIMACMAITNLTAILLLSPVVYTLASDYLRQRKLGVRPQFDPRRFPDIEPQLAPDTWD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
AASRD
CCCCC
>Mature Secondary Structure 
PASTRGTLMPEFFSFINEILWGSVMIYLLLGAGCWFTWRTGFIQFRYIRQFGRSLKGSL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
SPQPSGLTSFQALCTSLAARIGSGNLAGVALAIAAGGPGAVFWMWVSAIIGMATSFAECS
CCCCCCHHHHHHHHHHHHHHHCCCCHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
LAQLYKERDPTGQFRGGPAWYMARGLGMRWMGVVFALFLLVAYGLIFNSVQANAVSRALH
HHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAFNIPPLISGIALAFCALLIIIRGIKGVARLMQWLIPLIALLWVAGSVFICLWHIEQMP
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVIASIVKSAFGWQEAAAGAAGYTLTQAITSGFQRGMFSNEAGMGSTPNAAAAATSYPPH
HHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCC
PVAQGIVQMIGVFSDTIIICTASAMIILLAGNHASHSSTEGIQLLQHAMVSLTGEWGASF
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHH
VALIVILFAFSSIVANYIYAENNLFFLRLHNAKAIWLLRLATLGMVIAGTLISFPLIWQL
HHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADMIMACMAITNLTAILLLSPVVYTLASDYLRQRKLGVRPQFDPRRFPDIEPQLAPDTWD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
AASRD
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503 [H]