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The map label for this gene is citF [H]

Identifier: 194735905

GI number: 194735905

Start: 757154

End: 758683

Strand: Reverse

Name: citF [H]

Synonym: SeSA_A0780

Alternate gene names: 194735905

Gene position: 758683-757154 (Counterclockwise)

Preceding gene: 194736633

Following gene: 194735423

Centisome position: 16.11

GC content: 58.3

Gene sequence:

>1530_bases
ATGACGCAGAAAAATGAATCTCAACGCCAGGATCGCGTAGCAGCCTGGAGCCGCCACGCTGAAAGCGAACTGAGCGCTTA
CCAAAGTGCGGCAAAATTGGATCTACAAGCGCAAAAACCGCGCGACCACAAACTGTGCGCCAGCCTGGAAGAGGCCATCC
GCCGTTCCGGTTTACGCGACGGCATGACGCTCTCTTTTCACCACGCGTTTCGCGGCGGCGATCTGACGATTAACCTGGTG
ATGGAAACCATCGCGAAGATGGGCTTCAAAAACCTGACCCTGGCCTCCAGTTCGCTAAGCGACTGCCATGCGCCGCTGGT
TGAACACATTCGTAACGGGGTGGTCAGTCGCATCTATACGTCAGGCTTGCGCGGCCCGCTGGCGGAAGAAATTTCTCGCG
GCCTGCTGGCTGAGCCGGTTCAGATTCACTCCCACGGCGGTCGTGTACATCTGGTACAAAGCGGCGAACTCAATATTGAT
GTTGCGTTTCTTGGCGTGCCCTCCTGCGATGAATTCGGTAATGCTAACGGTTATACCGGCAAGGCCTGCTGCGGTTCACT
GGGCTACGCGATGGTGGATGCCGACAGCGCGAAACAGGTCGTAATGCTGACGGAGCAACTGCTGCCGTATCCGCATAACC
CGGCAAGCATCGCTCAGGATCAGGTTGATCTGATCGTGCAAATCGAAGAAGTCGGTGATGCAAACAAAATCGGCGCTGGC
GCGACCCGCATGACCACTAATCCGCGTGAACTGCTGATCGCCCGCAGCGCGGCGGAAGTCATTGCTCACTCCGGCTACTT
CAACGAAGGTTTCTCCCTACAAACCGGCACCGGCGGCGCATCGCTGGCGGTCACCCGCTTCCTGGAAGACAAAATGCGTA
GCCGCAACATTGTGGCTAATTTCGCGCTGGGCGGAATCACCGCCACCATGGTCGACCTGCATGAAAAGGGTTTAATCCGC
AAACTGCTTGACGTGCAGAGCTTCGATCGCAACGCTGCGCAATCTTTGGCCCGCAACCCGAACCATATCGAAATCAGCGC
TAACCAGTACGCCAACTGGGGCTCTAAAGGCGCTTCCGTCGACCGTCTGGACGTGGTGGTACTGAGCGCGCTGGAAGTTG
ACACCCACTTTAATGTTAACGTGCTGACCGGCTCCGACGGCGTTCTACGCGGCGCGTCCGGCGGTCACTGCGATACCGCC
GTCGCGGCGGCGCTGTCCATCATCGTCGCGCCGCTGGTGCGCGGTCGCATCCCAACGCTGGTTGATAACGTCACCACCTG
CGTCACACCAGGCTCCAGCGTTGACATTCTGGTCACCGATCACGGTATCGCCGTCAATCCGGCGCGTCCGGAGCTGGCTG
AACGCCTGAAAGCAGCGGGAATGAAAGTGGTATCGATTGAGTGGCTGCGCGAACGCGCGCAACTGCTTACCGGTCAGCCC
CGCCCTATCGAATTCACTGACCGCGTGATTGCTGTCGTGCGTTATCGCGACGGTTCAGTGATCGATGTGGTGCATCAGGT
GAAGGAATAA

Upstream 100 bases:

>100_bases
CCTTAACGGCAAGATGGTCGACAGTCCGGTGATTGAACGCGCCCGTCTGGTGCTCTCCCGTGCAGAACTTTCCGGTATTC
GCGAAGAATAAGGCAAAACA

Downstream 100 bases:

>100_bases
GCCATGCGCCTGCTTCCGGAACTCGCCACCTGTCACGACGTTTCTATTCCTGAGCTGCTCGCCAGCCGGGATGAGAGACA
AGCAAGACAGCGCGCCTGGC

Product: citrate lyase, alpha subunit

Products: NA

Alternate protein names: Citrase alpha chain; Citrate (pro-3S)-lyase alpha chain; Citrate CoA-transferase subunit [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MTQKNESQRQDRVAAWSRHAESELSAYQSAAKLDLQAQKPRDHKLCASLEEAIRRSGLRDGMTLSFHHAFRGGDLTINLV
METIAKMGFKNLTLASSSLSDCHAPLVEHIRNGVVSRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNID
VAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADSAKQVVMLTEQLLPYPHNPASIAQDQVDLIVQIEEVGDANKIGAG
ATRMTTNPRELLIARSAAEVIAHSGYFNEGFSLQTGTGGASLAVTRFLEDKMRSRNIVANFALGGITATMVDLHEKGLIR
KLLDVQSFDRNAAQSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEVDTHFNVNVLTGSDGVLRGASGGHCDTA
VAAALSIIVAPLVRGRIPTLVDNVTTCVTPGSSVDILVTDHGIAVNPARPELAERLKAAGMKVVSIEWLRERAQLLTGQP
RPIEFTDRVIAVVRYRDGSVIDVVHQVKE

Sequences:

>Translated_509_residues
MTQKNESQRQDRVAAWSRHAESELSAYQSAAKLDLQAQKPRDHKLCASLEEAIRRSGLRDGMTLSFHHAFRGGDLTINLV
METIAKMGFKNLTLASSSLSDCHAPLVEHIRNGVVSRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNID
VAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADSAKQVVMLTEQLLPYPHNPASIAQDQVDLIVQIEEVGDANKIGAG
ATRMTTNPRELLIARSAAEVIAHSGYFNEGFSLQTGTGGASLAVTRFLEDKMRSRNIVANFALGGITATMVDLHEKGLIR
KLLDVQSFDRNAAQSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEVDTHFNVNVLTGSDGVLRGASGGHCDTA
VAAALSIIVAPLVRGRIPTLVDNVTTCVTPGSSVDILVTDHGIAVNPARPELAERLKAAGMKVVSIEWLRERAQLLTGQP
RPIEFTDRVIAVVRYRDGSVIDVVHQVKE
>Mature_508_residues
TQKNESQRQDRVAAWSRHAESELSAYQSAAKLDLQAQKPRDHKLCASLEEAIRRSGLRDGMTLSFHHAFRGGDLTINLVM
ETIAKMGFKNLTLASSSLSDCHAPLVEHIRNGVVSRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNIDV
AFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADSAKQVVMLTEQLLPYPHNPASIAQDQVDLIVQIEEVGDANKIGAGA
TRMTTNPRELLIARSAAEVIAHSGYFNEGFSLQTGTGGASLAVTRFLEDKMRSRNIVANFALGGITATMVDLHEKGLIRK
LLDVQSFDRNAAQSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEVDTHFNVNVLTGSDGVLRGASGGHCDTAV
AAALSIIVAPLVRGRIPTLVDNVTTCVTPGSSVDILVTDHGIAVNPARPELAERLKAAGMKVVSIEWLRERAQLLTGQPR
PIEFTDRVIAVVRYRDGSVIDVVHQVKE

Specific function: Represents a citrate:acetyl-ACP transferase [H]

COG id: COG3051

COG function: function code C; Citrate lyase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786832, Length=510, Percent_Identity=88.4313725490196, Blast_Score=936, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006472 [H]

Pfam domain/function: PF04223 CitF [H]

EC number: =4.1.3.6; =2.8.3.10 [H]

Molecular weight: Translated: 54753; Mature: 54622

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKNESQRQDRVAAWSRHAESELSAYQSAAKLDLQAQKPRDHKLCASLEEAIRRSGLRD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCC
GMTLSFHHAFRGGDLTINLVMETIAKMGFKNLTLASSSLSDCHAPLVEHIRNGVVSRIYT
CCEEEEEEEECCCCEEHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
SGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNIDVAFLGVPSCDEFGNANGYTG
HCCCCCHHHHHHCCHHCCCCEEECCCCEEEEEECCCEEEEEEEEECCCCHHCCCCCCCCC
KACCGSLGYAMVDADSAKQVVMLTEQLLPYPHNPASIAQDQVDLIVQIEEVGDANKIGAG
HHHHHHCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHCCCEEEEEEECCCCCCCCCCC
ATRMTTNPRELLIARSAAEVIAHSGYFNEGFSLQTGTGGASLAVTRFLEDKMRSRNIVAN
CCCCCCCHHHHEEEHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEE
FALGGITATMVDLHEKGLIRKLLDVQSFDRNAAQSLARNPNHIEISANQYANWGSKGASV
EHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCC
DRLDVVVLSALEVDTHFNVNVLTGSDGVLRGASGGHCDTAVAAALSIIVAPLVRGRIPTL
CHHHHHHHHHEEECCCEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHH
VDNVTTCVTPGSSVDILVTDHGIAVNPARPELAERLKAAGMKVVSIEWLRERAQLLTGQP
HHCCHHEECCCCCEEEEEECCCEEECCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCC
RPIEFTDRVIAVVRYRDGSVIDVVHQVKE
CCCCHHCEEEEEEEECCCCHHHHHHHHCC
>Mature Secondary Structure 
TQKNESQRQDRVAAWSRHAESELSAYQSAAKLDLQAQKPRDHKLCASLEEAIRRSGLRD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCC
GMTLSFHHAFRGGDLTINLVMETIAKMGFKNLTLASSSLSDCHAPLVEHIRNGVVSRIYT
CCEEEEEEEECCCCEEHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
SGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNIDVAFLGVPSCDEFGNANGYTG
HCCCCCHHHHHHCCHHCCCCEEECCCCEEEEEECCCEEEEEEEEECCCCHHCCCCCCCCC
KACCGSLGYAMVDADSAKQVVMLTEQLLPYPHNPASIAQDQVDLIVQIEEVGDANKIGAG
HHHHHHCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHCCCEEEEEEECCCCCCCCCCC
ATRMTTNPRELLIARSAAEVIAHSGYFNEGFSLQTGTGGASLAVTRFLEDKMRSRNIVAN
CCCCCCCHHHHEEEHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEE
FALGGITATMVDLHEKGLIRKLLDVQSFDRNAAQSLARNPNHIEISANQYANWGSKGASV
EHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCC
DRLDVVVLSALEVDTHFNVNVLTGSDGVLRGASGGHCDTAVAAALSIIVAPLVRGRIPTL
CHHHHHHHHHEEECCCEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHH
VDNVTTCVTPGSSVDILVTDHGIAVNPARPELAERLKAAGMKVVSIEWLRERAQLLTGQP
HHCCHHEECCCCCEEEEEECCCEEECCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCC
RPIEFTDRVIAVVRYRDGSVIDVVHQVKE
CCCCHHCEEEEEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]