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The map label for this gene is bcsZ [H]

Identifier: 194735770

GI number: 194735770

Start: 3681391

End: 3682500

Strand: Reverse

Name: bcsZ [H]

Synonym: SeSA_A3812

Alternate gene names: 194735770

Gene position: 3682500-3681391 (Counterclockwise)

Preceding gene: 194736676

Following gene: 194735245

Centisome position: 78.2

GC content: 56.49

Gene sequence:

>1110_bases
ATGATGACTATGCTGCGCGGATGGATAACGATGCTCGTCATGCTGACGGCAATAAATGCGCAGGCAGCCTGTAGCTGGCC
TGCGTGGGAACAGTTCAAGAAAGATTACATTAGCCAGCAGGGACGCGTTATCGATCCGGGCGATGCGCGAAAAATTACCA
CCTCCGAAGGGCAAAGCTACGCCATGTTCTTTGCCCTGGCAGCGAACGATCGACCGGCGTTCGCGCAACTGTTTAACTGG
ACGCAAAACAATCTGGCGCAGGGATCGCTGCGTGAACATCTGCCCGCCTGGCTGTGGGGGCAAAAAGATCCCGACACCTG
GTCGGTGCTGGACAGCAACTCCGCGTCCGACGGCGATATCTGGATGGCATGGTCGCTGCTGGAGGCCGGTCGTCTGTGGA
AAGAGACGCGTTATACCGAGGTGGGCACGGCGTTGCTAAAACGCATCGCCCGCGAAGAGGTCGTGAATGTGCCGGGGCTG
GGCTCAATGCTGCTACCTGGCAAAATCGGCTTTGCCGAGGCGAATAGCTGGCGTTTTAACCCAAGCTATCTGCCGCCGCA
GTTGGCGCAATACTTTAGCCGTTTTGGCGCGCCGTGGTCGACGCTACGGGAAACCAATTTGCGGCTTTTGCTGGAAACCG
CGCCGAAAGGTTTCTCGCCGGACTGGGTGCGTTATGAAAGCAAGCAAGGCTGGCAGTTGAAAGCGGAAAAGACGCTGATC
AGTAGCTACGATGCGATTCGCGTCTATTTATGGGCGGGAATGATGCATGATGGCGATCCGCAAAAAGCGCGTTTACTGGC
GCGATTTAAACCGATGGCGACGTTAACGATGAAAAACGGCGTTCCACCGGAGAAAGTGGATGTCGTCAGCGGGAACGCGC
AAGGGACGGGGCCGGTCGGGTTTTCCGCCGCCTTACTGCCTTTCCTGCAAAATCGCGACGCCCAGGCCGTGCAGCGACAG
CGGGTCGCAGACCATTTTCCTGGCAGCGATGCCTATTACAACTATGTGCTGACTCTCTTTGGACAAGGCTGGGATCAGCA
CCGTTTTCGCTTCACCGTCAAAGGTGAATTATTACCTGACTGGGGCCAGGAATGCGTAAGTTCACGTTAA

Upstream 100 bases:

>100_bases
GGTACTGGCGGCCCTCAGTGTGGTATTACTGGCGTGGGTATTGTGGCGTCTGCTACGTATTCTCAGTCGCCGTCGTCTCG
ACCCTGACCATGAGTAACGC

Downstream 100 bases:

>100_bases
GTCTCATGCACGCTTTTTTGCCGGCTGGCGGTCGAAATGCCTTACCTGGCAAAAGGGGCGTTTCTCGGGCATTGTTGGGT
TTATCGCTTGGTATGGCGCT

Product: endo-1,4-D-glucanase

Products: NA

Alternate protein names: Carboxymethylcellulase; CMCase; Cellulase; Endo-1,4-beta-glucanase [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MMTMLRGWITMLVMLTAINAQAACSWPAWEQFKKDYISQQGRVIDPGDARKITTSEGQSYAMFFALAANDRPAFAQLFNW
TQNNLAQGSLREHLPAWLWGQKDPDTWSVLDSNSASDGDIWMAWSLLEAGRLWKETRYTEVGTALLKRIAREEVVNVPGL
GSMLLPGKIGFAEANSWRFNPSYLPPQLAQYFSRFGAPWSTLRETNLRLLLETAPKGFSPDWVRYESKQGWQLKAEKTLI
SSYDAIRVYLWAGMMHDGDPQKARLLARFKPMATLTMKNGVPPEKVDVVSGNAQGTGPVGFSAALLPFLQNRDAQAVQRQ
RVADHFPGSDAYYNYVLTLFGQGWDQHRFRFTVKGELLPDWGQECVSSR

Sequences:

>Translated_369_residues
MMTMLRGWITMLVMLTAINAQAACSWPAWEQFKKDYISQQGRVIDPGDARKITTSEGQSYAMFFALAANDRPAFAQLFNW
TQNNLAQGSLREHLPAWLWGQKDPDTWSVLDSNSASDGDIWMAWSLLEAGRLWKETRYTEVGTALLKRIAREEVVNVPGL
GSMLLPGKIGFAEANSWRFNPSYLPPQLAQYFSRFGAPWSTLRETNLRLLLETAPKGFSPDWVRYESKQGWQLKAEKTLI
SSYDAIRVYLWAGMMHDGDPQKARLLARFKPMATLTMKNGVPPEKVDVVSGNAQGTGPVGFSAALLPFLQNRDAQAVQRQ
RVADHFPGSDAYYNYVLTLFGQGWDQHRFRFTVKGELLPDWGQECVSSR
>Mature_369_residues
MMTMLRGWITMLVMLTAINAQAACSWPAWEQFKKDYISQQGRVIDPGDARKITTSEGQSYAMFFALAANDRPAFAQLFNW
TQNNLAQGSLREHLPAWLWGQKDPDTWSVLDSNSASDGDIWMAWSLLEAGRLWKETRYTEVGTALLKRIAREEVVNVPGL
GSMLLPGKIGFAEANSWRFNPSYLPPQLAQYFSRFGAPWSTLRETNLRLLLETAPKGFSPDWVRYESKQGWQLKAEKTLI
SSYDAIRVYLWAGMMHDGDPQKARLLARFKPMATLTMKNGVPPEKVDVVSGNAQGTGPVGFSAALLPFLQNRDAQAVQRQ
RVADHFPGSDAYYNYVLTLFGQGWDQHRFRFTVKGELLPDWGQECVSSR

Specific function: Hydrolyzes carboxymethylcellulose [H]

COG id: COG3405

COG function: function code G; Endoglucanase Y

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 8 (cellulase D) family [H]

Homologues:

Organism=Escherichia coli, GI1789951, Length=368, Percent_Identity=81.7934782608696, Blast_Score=640, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR002037
- InterPro:   IPR019834 [H]

Pfam domain/function: PF01270 Glyco_hydro_8 [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 41715; Mature: 41715

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS00812 GLYCOSYL_HYDROL_F8

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTMLRGWITMLVMLTAINAQAACSWPAWEQFKKDYISQQGRVIDPGDARKITTSEGQSY
CHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCCEEECCCCCEE
AMFFALAANDRPAFAQLFNWTQNNLAQGSLREHLPAWLWGQKDPDTWSVLDSNSASDGDI
EEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHCCCHHHCCCCCCCCEEEECCCCCCCCCE
WMAWSLLEAGRLWKETRYTEVGTALLKRIAREEVVNVPGLGSMLLPGKIGFAEANSWRFN
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
PSYLPPQLAQYFSRFGAPWSTLRETNLRLLLETAPKGFSPDWVRYESKQGWQLKAEKTLI
CCCCCHHHHHHHHHHCCCHHHHHHCCEEEEEEECCCCCCCCCEEECCCCCCEEEHHHHHH
SSYDAIRVYLWAGMMHDGDPQKARLLARFKPMATLTMKNGVPPEKVDVVSGNAQGTGPVG
HHHHHHEEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCCCCC
FSAALLPFLQNRDAQAVQRQRVADHFPGSDAYYNYVLTLFGQGWDQHRFRFTVKGELLPD
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCH
WGQECVSSR
HHHHHHCCC
>Mature Secondary Structure
MMTMLRGWITMLVMLTAINAQAACSWPAWEQFKKDYISQQGRVIDPGDARKITTSEGQSY
CHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCCEEECCCCCEE
AMFFALAANDRPAFAQLFNWTQNNLAQGSLREHLPAWLWGQKDPDTWSVLDSNSASDGDI
EEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHCCCHHHCCCCCCCCEEEECCCCCCCCCE
WMAWSLLEAGRLWKETRYTEVGTALLKRIAREEVVNVPGLGSMLLPGKIGFAEANSWRFN
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
PSYLPPQLAQYFSRFGAPWSTLRETNLRLLLETAPKGFSPDWVRYESKQGWQLKAEKTLI
CCCCCHHHHHHHHHHCCCHHHHHHCCEEEEEEECCCCCCCCCEEECCCCCCEEEHHHHHH
SSYDAIRVYLWAGMMHDGDPQKARLLARFKPMATLTMKNGVPPEKVDVVSGNAQGTGPVG
HHHHHHEEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCCCCC
FSAALLPFLQNRDAQAVQRQRVADHFPGSDAYYNYVLTLFGQGWDQHRFRFTVKGELLPD
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCH
WGQECVSSR
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]