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The map label for this gene is menE [H]

Identifier: 194735721

GI number: 194735721

Start: 2071756

End: 2073252

Strand: Reverse

Name: menE [H]

Synonym: SeSA_A2181

Alternate gene names: 194735721

Gene position: 2073252-2071756 (Counterclockwise)

Preceding gene: 194736607

Following gene: 194735050

Centisome position: 44.03

GC content: 40.55

Gene sequence:

>1497_bases
ATGATTACTCTTCGCAGACTGAATGAAATAGCCATTACTCGAGGTAATGATATTTGCATTATTGATAAAGAACGACAATA
TACGTGGTATGATATTATTCGCCGTACTGAGTCTCGCATTGTCTTTCTCCGTCGGGCATTTAACCCTGAGCAGTTACGGT
CTGTTTGCTATTTATCAAAAAATAGTGTCGATCTAATTTGTTGGTTGGCTGCTTTTGCTACTTTAGGAATTCCTGCAAAC
GGTCTTGACTACTCATTACCTATTGAGACCTTGAGAGGGTTGCTAATTAAAATTAATCCGGGACTAATGTTAGTCTCTTT
TAGCCTTTACTCTCCGGATGAACTCAATAAGTTGCATGTCCGTACTATAACCATGCTGGCAGTCGATGCTCCAACTGATC
CAGTTATTGGCAGTATAGGTGAATTTCATCACCCTGAATTGGAAAGTCTACTTGCGACACATATTCCTGCACCATTTCGC
TCAGTTTCACTAACTTCAGGAACCAGTTCAGCACCTAAAATCGTATTACGCTACAACTCTTTTGATGCCCGGCGTTTCGA
CTGGTTTACTCAGCGATTCAACTTCACGCACCACGATGGTTTCCTGCTTATCTTACCCCTTTATCATGCAGCAGGAAATG
GCTGGGCAAGGATGTTTATGGGGCTTGGGGCTTCTTTACATTTGGTGGATCAGGATGATGAATCTGCTCTTATTCAGGCA
CTTTCTCTCAACTCCGTAAAGGCTACAGTGATGACACCTAATCTGGTTTCCCGTCTCACTAAACTCGCCAGTGAAACAGT
TTTACATCATTACTTGCGTTGGGTTTTGGTTGGTGGAAGCTATTTTCCAGTAAAAAGCAAAATAGCTGCATATACCCACT
TGGGACATATTTTTAACGAGTATTACGGGTGTACCGAAACTGGGGTGAACGTTCTTTCTGAGTCTTCAGATATGTTTGAG
TGTCCAGGTAGCGTGGGGAGAGCCTTTGACGGTAATAAAATCAGAATATTGGATGAGGATAATGTACCACTGAAAGCAGG
CAATAGGGGAAGAATAGCTGTAGCCAGTTATATGCTTATGGATGAATACGGTGATGGCAGTAGGCCTTTCATTGAAATAG
ACAGTGAACGCTATTTTTTGATGGCTGACTACGGATATCTTGACGATAATGGACGACTATTCTTGATGAATCGTAATAGT
GAAATAAAGTGTGAGCAAGACATTTATCATATTGAGGAACATTTACGTGCACTCCCCTGTATTACAGATGTGGCACTCAT
ACCAATAAGACAACAAAATAAAGATCATATCAGGTGCATATTTAGCGCTAAACATATAAATGAAGATGATGTTTCTTTTA
TTATGGATGAAATAAAAAATAAAATTAATCATATCGGGGTGACTGATTTCACCGCACATATGGTTGATAAAATACCTTAT
AGCCCGAGTGGGAAAGTCCGTTTCAGCGAGATAGTCCAAACTCTGACAGCAGCATAA

Upstream 100 bases:

>100_bases
AGCAAAGACGTTAGAAATAAAATAAATGAATGTAAACGGAATGGCTGCCTAATTATTGCTGCCTGATTATATAATTTATA
CTATCTAAGGAATATCTAAA

Downstream 100 bases:

>100_bases
GTATCCGATATCATAATTTCTTATATAGATATCAATATTAACGAATGGCTATTTTTGAAGTGTTGTGAAAATGTAAGATA
CAACATCCATGATAACTATC

Product: putative AMP-binding enzyme

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MITLRRLNEIAITRGNDICIIDKERQYTWYDIIRRTESRIVFLRRAFNPEQLRSVCYLSKNSVDLICWLAAFATLGIPAN
GLDYSLPIETLRGLLIKINPGLMLVSFSLYSPDELNKLHVRTITMLAVDAPTDPVIGSIGEFHHPELESLLATHIPAPFR
SVSLTSGTSSAPKIVLRYNSFDARRFDWFTQRFNFTHHDGFLLILPLYHAAGNGWARMFMGLGASLHLVDQDDESALIQA
LSLNSVKATVMTPNLVSRLTKLASETVLHHYLRWVLVGGSYFPVKSKIAAYTHLGHIFNEYYGCTETGVNVLSESSDMFE
CPGSVGRAFDGNKIRILDEDNVPLKAGNRGRIAVASYMLMDEYGDGSRPFIEIDSERYFLMADYGYLDDNGRLFLMNRNS
EIKCEQDIYHIEEHLRALPCITDVALIPIRQQNKDHIRCIFSAKHINEDDVSFIMDEIKNKINHIGVTDFTAHMVDKIPY
SPSGKVRFSEIVQTLTAA

Sequences:

>Translated_498_residues
MITLRRLNEIAITRGNDICIIDKERQYTWYDIIRRTESRIVFLRRAFNPEQLRSVCYLSKNSVDLICWLAAFATLGIPAN
GLDYSLPIETLRGLLIKINPGLMLVSFSLYSPDELNKLHVRTITMLAVDAPTDPVIGSIGEFHHPELESLLATHIPAPFR
SVSLTSGTSSAPKIVLRYNSFDARRFDWFTQRFNFTHHDGFLLILPLYHAAGNGWARMFMGLGASLHLVDQDDESALIQA
LSLNSVKATVMTPNLVSRLTKLASETVLHHYLRWVLVGGSYFPVKSKIAAYTHLGHIFNEYYGCTETGVNVLSESSDMFE
CPGSVGRAFDGNKIRILDEDNVPLKAGNRGRIAVASYMLMDEYGDGSRPFIEIDSERYFLMADYGYLDDNGRLFLMNRNS
EIKCEQDIYHIEEHLRALPCITDVALIPIRQQNKDHIRCIFSAKHINEDDVSFIMDEIKNKINHIGVTDFTAHMVDKIPY
SPSGKVRFSEIVQTLTAA
>Mature_498_residues
MITLRRLNEIAITRGNDICIIDKERQYTWYDIIRRTESRIVFLRRAFNPEQLRSVCYLSKNSVDLICWLAAFATLGIPAN
GLDYSLPIETLRGLLIKINPGLMLVSFSLYSPDELNKLHVRTITMLAVDAPTDPVIGSIGEFHHPELESLLATHIPAPFR
SVSLTSGTSSAPKIVLRYNSFDARRFDWFTQRFNFTHHDGFLLILPLYHAAGNGWARMFMGLGASLHLVDQDDESALIQA
LSLNSVKATVMTPNLVSRLTKLASETVLHHYLRWVLVGGSYFPVKSKIAAYTHLGHIFNEYYGCTETGVNVLSESSDMFE
CPGSVGRAFDGNKIRILDEDNVPLKAGNRGRIAVASYMLMDEYGDGSRPFIEIDSERYFLMADYGYLDDNGRLFLMNRNS
EIKCEQDIYHIEEHLRALPCITDVALIPIRQQNKDHIRCIFSAKHINEDDVSFIMDEIKNKINHIGVTDFTAHMVDKIPY
SPSGKVRFSEIVQTLTAA

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 56347; Mature: 56347

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLRRLNEIAITRGNDICIIDKERQYTWYDIIRRTESRIVFLRRAFNPEQLRSVCYLSK
CEEHHCCCEEEEECCCEEEEEECCCCCHHHHHHHHCCHHEEEEECCCCHHHHHHHHHCCC
NSVDLICWLAAFATLGIPANGLDYSLPIETLRGLLIKINPGLMLVSFSLYSPDELNKLHV
CCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEEECCCEEEEEEECCCCCCCCEEEE
RTITMLAVDAPTDPVIGSIGEFHHPELESLLATHIPAPFRSVSLTSGTSSAPKIVLRYNS
EEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEECC
FDARRFDWFTQRFNFTHHDGFLLILPLYHAAGNGWARMFMGLGASLHLVDQDDESALIQA
CCCHHHHHHHHHCCCEECCCEEEEEEEHHCCCCHHHHHHHHCCCEEEEECCCCHHHHHHH
LSLNSVKATVMTPNLVSRLTKLASETVLHHYLRWVLVGGSYFPVKSKIAAYTHLGHIFNE
HHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHH
YYGCTETGVNVLSESSDMFECPGSVGRAFDGNKIRILDEDNVPLKAGNRGRIAVASYMLM
HCCCCHHHHHHHCCCCCCEECCCHHCCCCCCCEEEEEECCCCCEECCCCCCEEEEEEEEH
DEYGDGSRPFIEIDSERYFLMADYGYLDDNGRLFLMNRNSEIKCEQDIYHIEEHLRALPC
HCCCCCCCCEEEECCCEEEEEEECCEECCCCEEEEEECCCCCEEHHHHHHHHHHHHHCCC
ITDVALIPIRQQNKDHIRCIFSAKHINEDDVSFIMDEIKNKINHIGVTDFTAHMVDKIPY
HHCEEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
SPSGKVRFSEIVQTLTAA
CCCCCEEHHHHHHHHHCC
>Mature Secondary Structure
MITLRRLNEIAITRGNDICIIDKERQYTWYDIIRRTESRIVFLRRAFNPEQLRSVCYLSK
CEEHHCCCEEEEECCCEEEEEECCCCCHHHHHHHHCCHHEEEEECCCCHHHHHHHHHCCC
NSVDLICWLAAFATLGIPANGLDYSLPIETLRGLLIKINPGLMLVSFSLYSPDELNKLHV
CCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEEECCCEEEEEEECCCCCCCCEEEE
RTITMLAVDAPTDPVIGSIGEFHHPELESLLATHIPAPFRSVSLTSGTSSAPKIVLRYNS
EEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEECC
FDARRFDWFTQRFNFTHHDGFLLILPLYHAAGNGWARMFMGLGASLHLVDQDDESALIQA
CCCHHHHHHHHHCCCEECCCEEEEEEEHHCCCCHHHHHHHHCCCEEEEECCCCHHHHHHH
LSLNSVKATVMTPNLVSRLTKLASETVLHHYLRWVLVGGSYFPVKSKIAAYTHLGHIFNE
HHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHH
YYGCTETGVNVLSESSDMFECPGSVGRAFDGNKIRILDEDNVPLKAGNRGRIAVASYMLM
HCCCCHHHHHHHCCCCCCEECCCHHCCCCCCCEEEEEECCCCCEECCCCCCEEEEEEEEH
DEYGDGSRPFIEIDSERYFLMADYGYLDDNGRLFLMNRNSEIKCEQDIYHIEEHLRALPC
HCCCCCCCCEEEECCCEEEEEEECCEECCCCEEEEEECCCCCEEHHHHHHHHHHHHHCCC
ITDVALIPIRQQNKDHIRCIFSAKHINEDDVSFIMDEIKNKINHIGVTDFTAHMVDKIPY
HHCEEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
SPSGKVRFSEIVQTLTAA
CCCCCEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]