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The map label for this gene is ybfF [H]

Identifier: 194735571

GI number: 194735571

Start: 824002

End: 824772

Strand: Reverse

Name: ybfF [H]

Synonym: SeSA_A0853

Alternate gene names: 194735571

Gene position: 824772-824002 (Counterclockwise)

Preceding gene: 194736782

Following gene: 194736295

Centisome position: 17.51

GC content: 56.16

Gene sequence:

>771_bases
ATGAAATTAAACATCCGAGCGCAATCTGCACAAAACCTGCACAATAATTCCCCCATCGTTCTCGTTCATGGCCTGTTTGG
CAGCCTGGACAACCTCGGCGTACTGGCGCGCGATCTGGTGACCGATCACGACATTATTCAGGTCGATATGCGCAATCATG
GTTTATCGCCGCGCGATCCGGTCATGGATTACCCGACAATGGCCCAGGATCTTCTGGATACGCTTGACGCGCAGCAGATT
GAAAAAGCGACGTTTATTGGTCATTCCATGGGTGGAAAAGCGGTAATGGCGCTGACGGCGCTGGCGCCCGATCGCATCGA
TCGTCTGGTCGCGATTGATATCGCACCCGTGGATTATCACGTTCGCCGCCACGATCGCATTTTCGCGGCTATTAACGCCG
TCAGCGAATCCGACGCGACATCCCGCCAACAGGCGGCAGGGATTATGCGCCAGCACCTCAACGAAGAAGGCGTGATCCAG
TTTCTGTTAAAATCCTGGGCTGAAGGCGAATGGCGTTTTAATGTTCCGGTATTATGGGAACAGTATCCGCATATCGTCGG
CTGGGAAACCATTCCGCCCTGGGAACATCCGGCGCTGTTTATTCCAGGCGGTAACTCGCCCTACGTGACCGAAGCGTACC
GCGACGCGCTGCTGGCGCAATTCCCTCTGGCGCGCGCGCATGTGATCGCCGGCGCGGGCCACTGGGTACATGCTGAAAAA
CCAGAGGCGGTTCTGCGCGCCATCCGCCGTTATCTGCACGATAAACGCTAA

Upstream 100 bases:

>100_bases
CTGACAAAAAAACTCGGAGTAAACCTTAGAGGTTGCAGCCCAGTGGCAGAATGGTCAACATAATACCCTAACCCGACAGA
GCGAAAAAAAGAACTTTGCC

Downstream 100 bases:

>100_bases
CTGATTAAAAACTCATCGCCTGTGCGCGATTACGCGCGCGGGCGTTGGCGTCGCTTCCTCGCTGATGTATGATGGCGCGC
TATCCATCCGGGCATAAGCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MKLNIRAQSAQNLHNNSPIVLVHGLFGSLDNLGVLARDLVTDHDIIQVDMRNHGLSPRDPVMDYPTMAQDLLDTLDAQQI
EKATFIGHSMGGKAVMALTALAPDRIDRLVAIDIAPVDYHVRRHDRIFAAINAVSESDATSRQQAAGIMRQHLNEEGVIQ
FLLKSWAEGEWRFNVPVLWEQYPHIVGWETIPPWEHPALFIPGGNSPYVTEAYRDALLAQFPLARAHVIAGAGHWVHAEK
PEAVLRAIRRYLHDKR

Sequences:

>Translated_256_residues
MKLNIRAQSAQNLHNNSPIVLVHGLFGSLDNLGVLARDLVTDHDIIQVDMRNHGLSPRDPVMDYPTMAQDLLDTLDAQQI
EKATFIGHSMGGKAVMALTALAPDRIDRLVAIDIAPVDYHVRRHDRIFAAINAVSESDATSRQQAAGIMRQHLNEEGVIQ
FLLKSWAEGEWRFNVPVLWEQYPHIVGWETIPPWEHPALFIPGGNSPYVTEAYRDALLAQFPLARAHVIAGAGHWVHAEK
PEAVLRAIRRYLHDKR
>Mature_256_residues
MKLNIRAQSAQNLHNNSPIVLVHGLFGSLDNLGVLARDLVTDHDIIQVDMRNHGLSPRDPVMDYPTMAQDLLDTLDAQQI
EKATFIGHSMGGKAVMALTALAPDRIDRLVAIDIAPVDYHVRRHDRIFAAINAVSESDATSRQQAAGIMRQHLNEEGVIQ
FLLKSWAEGEWRFNVPVLWEQYPHIVGWETIPPWEHPALFIPGGNSPYVTEAYRDALLAQFPLARAHVIAGAGHWVHAEK
PEAVLRAIRRYLHDKR

Specific function: Displays esterase activity toward palmitoyl-CoA, malonyl-CoA and pNP-butyrate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200012, Length=246, Percent_Identity=34.5528455284553, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI23200008, Length=246, Percent_Identity=34.5528455284553, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI223941839, Length=237, Percent_Identity=30.379746835443, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1786902, Length=254, Percent_Identity=89.3700787401575, Blast_Score=471, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17508535, Length=264, Percent_Identity=32.9545454545455, Blast_Score=133, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17507063, Length=252, Percent_Identity=26.5873015873016, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321468, Length=261, Percent_Identity=31.4176245210728, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24640348, Length=270, Percent_Identity=27.7777777777778, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24646055, Length=274, Percent_Identity=26.6423357664234, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 28737; Mature: 28737

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNIRAQSAQNLHNNSPIVLVHGLFGSLDNLGVLARDLVTDHDIIQVDMRNHGLSPRDP
CEEEEECHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEHHHCCCCCCCC
VMDYPTMAQDLLDTLDAQQIEKATFIGHSMGGKAVMALTALAPDRIDRLVAIDIAPVDYH
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHCEEEEEEEECCCHH
VRRHDRIFAAINAVSESDATSRQQAAGIMRQHLNEEGVIQFLLKSWAEGEWRFNVPVLWE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCCHHH
QYPHIVGWETIPPWEHPALFIPGGNSPYVTEAYRDALLAQFPLARAHVIAGAGHWVHAEK
CCCCEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHEEECCCCEEECCC
PEAVLRAIRRYLHDKR
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLNIRAQSAQNLHNNSPIVLVHGLFGSLDNLGVLARDLVTDHDIIQVDMRNHGLSPRDP
CEEEEECHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEHHHCCCCCCCC
VMDYPTMAQDLLDTLDAQQIEKATFIGHSMGGKAVMALTALAPDRIDRLVAIDIAPVDYH
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHCEEEEEEEECCCHH
VRRHDRIFAAINAVSESDATSRQQAAGIMRQHLNEEGVIQFLLKSWAEGEWRFNVPVLWE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCCHHH
QYPHIVGWETIPPWEHPALFIPGGNSPYVTEAYRDALLAQFPLARAHVIAGAGHWVHAEK
CCCCEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHEEECCCCEEECCC
PEAVLRAIRRYLHDKR
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]