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The map label for this gene is pduF

Identifier: 194735513

GI number: 194735513

Start: 2095056

End: 2095850

Strand: Reverse

Name: pduF

Synonym: SeSA_A2207

Alternate gene names: 194735513

Gene position: 2095850-2095056 (Counterclockwise)

Preceding gene: 194737526

Following gene: 194736794

Centisome position: 44.51

GC content: 50.69

Gene sequence:

>795_bases
ATGAATGATTCTCTAAAGGCGCAATGTGGTGCGGAATTTTTAGGCACCGGACTCTTTTTGTTTTTTGGTATCGGTTGTTT
GAGTGCGCTCAAAGTCGCAGGCGCCAGCCTCGGATTATGGGAGATTTGCATTATTTGGGGGCTTGGAATTTCACTGGCCG
TTTACCTTACGGCCGGAATCTCCGGCGGACATCTTAACCCTGCGGTGACGATTGCGCTGTGGCTGTTTGCCTGCTTTCCC
AAACAGAAAGTATTGCCTTATATTATTGCCCAGTTTGCCGGCGCTTTTGGCGGCGCTCTGCTGGCGTATGTGCTTTATAG
TAGCCTTTTTACTGAATTTGAGACTGCGCATCATATGGTGCGCGGTAGCGTCGAAAGTTTGCAATTAGCCAGCATTTTCA
GTACCTATCCGGCCGCAGCGCTAAATGTATGGCAGGCTGCGTTGGTGGAAGTGGTCATTACGTCGATTCTGATGGGCATG
ATCATGGCATTGACGGATGATGGCAATGGCATTCCTAAAGGGCCGCTTGCGCCTTTGCTTATTGGGATATTGGTTGCCGT
TATCGGCGCGTCTACTGGACCCCTTACCGGTTTTGCAATGAACCCGGCGCGTGATTTCGGTCCCAAACTGTTCACATGGT
TAGCGGGGTGGGGGAACATGGCGATGAGCGGTGGGCGAGAGATCCCTTATTTTATCGTGCCGATTGTCGCGCCTGTTATC
GGCGCCTGTGCCGGAGCAGCCATATATCGCTATTTTATTGGTAAGAATTTACCTTGTAACCGCTGTGAACTGTAA

Upstream 100 bases:

>100_bases
CAATTTACAGGCGGCACTTTTTATAGTGAGGTTAGCTGTACTCTACTGCCAGCCGAAACTTGCGCTGGCCAGGACACGAA
CTTTGCTCAGAAGGTGTCAC

Downstream 100 bases:

>100_bases
GAAAATGCGAACCAGATTCACAGCAATGATGTATCGTAAAAACAGGTGAGATAATTTCGTGATCTGCTTTCACGAACTGA
TCAAAACGTCTCCCAGCAAT

Product: propanediol diffusion facilitator

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MNDSLKAQCGAEFLGTGLFLFFGIGCLSALKVAGASLGLWEICIIWGLGISLAVYLTAGISGGHLNPAVTIALWLFACFP
KQKVLPYIIAQFAGAFGGALLAYVLYSSLFTEFETAHHMVRGSVESLQLASIFSTYPAAALNVWQAALVEVVITSILMGM
IMALTDDGNGIPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKLFTWLAGWGNMAMSGGREIPYFIVPIVAPVI
GACAGAAIYRYFIGKNLPCNRCEL

Sequences:

>Translated_264_residues
MNDSLKAQCGAEFLGTGLFLFFGIGCLSALKVAGASLGLWEICIIWGLGISLAVYLTAGISGGHLNPAVTIALWLFACFP
KQKVLPYIIAQFAGAFGGALLAYVLYSSLFTEFETAHHMVRGSVESLQLASIFSTYPAAALNVWQAALVEVVITSILMGM
IMALTDDGNGIPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKLFTWLAGWGNMAMSGGREIPYFIVPIVAPVI
GACAGAAIYRYFIGKNLPCNRCEL
>Mature_264_residues
MNDSLKAQCGAEFLGTGLFLFFGIGCLSALKVAGASLGLWEICIIWGLGISLAVYLTAGISGGHLNPAVTIALWLFACFP
KQKVLPYIIAQFAGAFGGALLAYVLYSSLFTEFETAHHMVRGSVESLQLASIFSTYPAAALNVWQAALVEVVITSILMGM
IMALTDDGNGIPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKLFTWLAGWGNMAMSGGREIPYFIVPIVAPVI
GACAGAAIYRYFIGKNLPCNRCEL

Specific function: May facilitate the diffusion of propanediol

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family

Homologues:

Organism=Homo sapiens, GI157266307, Length=260, Percent_Identity=37.6923076923077, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI4502187, Length=257, Percent_Identity=36.1867704280156, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI22538420, Length=256, Percent_Identity=35.546875, Blast_Score=143, Evalue=1e-34,
Organism=Homo sapiens, GI4826645, Length=266, Percent_Identity=36.4661654135338, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI310133356, Length=218, Percent_Identity=32.1100917431193, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI310114181, Length=218, Percent_Identity=32.1100917431193, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI45446752, Length=202, Percent_Identity=35.1485148514851, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI4502183, Length=246, Percent_Identity=32.520325203252, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4502179, Length=244, Percent_Identity=31.9672131147541, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI86792455, Length=202, Percent_Identity=33.6633663366337, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI6912506, Length=247, Percent_Identity=30.3643724696356, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1790362, Length=262, Percent_Identity=68.3206106870229, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI1787101, Length=220, Percent_Identity=31.3636363636364, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71992966, Length=245, Percent_Identity=37.9591836734694, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71994009, Length=252, Percent_Identity=36.1111111111111, Blast_Score=141, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17544068, Length=246, Percent_Identity=39.0243902439024, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32564052, Length=252, Percent_Identity=34.9206349206349, Blast_Score=127, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17533613, Length=252, Percent_Identity=34.9206349206349, Blast_Score=127, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17531429, Length=254, Percent_Identity=31.8897637795276, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17531431, Length=254, Percent_Identity=31.8897637795276, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71992961, Length=245, Percent_Identity=35.1020408163265, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71993722, Length=253, Percent_Identity=27.6679841897233, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17558372, Length=260, Percent_Identity=27.3076923076923, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI212646268, Length=236, Percent_Identity=30.0847457627119, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=212, Percent_Identity=41.5094339622642, Blast_Score=143, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6322985, Length=233, Percent_Identity=30.9012875536481, Blast_Score=89, Evalue=5e-19,
Organism=Drosophila melanogaster, GI45550503, Length=248, Percent_Identity=26.2096774193548, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24762344, Length=249, Percent_Identity=28.9156626506024, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24762346, Length=249, Percent_Identity=28.9156626506024, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24762348, Length=249, Percent_Identity=28.9156626506024, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI20130305, Length=249, Percent_Identity=28.9156626506024, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24652747, Length=208, Percent_Identity=28.3653846153846, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI45551084, Length=208, Percent_Identity=28.3653846153846, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDUF_SALTY (P37451)

Other databases:

- EMBL:   AF026270
- EMBL:   AE006468
- RefSeq:   NP_460982.1
- ProteinModelPortal:   P37451
- SMR:   P37451
- GeneID:   1253558
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2037
- NMPDR:   fig|99287.1.peg.1962
- HOGENOM:   HBG705794
- OMA:   EVVITSM
- ProtClustDB:   CLSK2393520
- BioCyc:   STYP99287:STM2037-MONOMER
- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357
- PANTHER:   PTHR19139
- PRINTS:   PR00783
- TIGRFAMs:   TIGR00861

Pfam domain/function: PF00230 MIP

EC number: NA

Molecular weight: Translated: 27744; Mature: 27744

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00221 MIP; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1f99f22c)-; HASH(0x1f935440)-; HASH(0x1f84be70)-; HASH(0x1f8ea254)-; HASH(0x1fda0dec)-; HASH(0x1f987048)-;

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCCCCCCCCCCH
>Mature Secondary Structure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HHHHHHHHHHHHHCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8071226; 9352910; 11677609