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The map label for this gene is rluF [H]

Identifier: 194735254

GI number: 194735254

Start: 4281454

End: 4282323

Strand: Direct

Name: rluF [H]

Synonym: SeSA_A4405

Alternate gene names: 194735254

Gene position: 4281454-4282323 (Clockwise)

Preceding gene: 194735823

Following gene: 194737197

Centisome position: 90.92

GC content: 50.8

Gene sequence:

>870_bases
ATGCTGACTGACACATCTACCCGGCTTAATAAATATATCAGTGAAAGCGGCATCTGTTCGCGTCGCGAGGCGGACCGTTT
TATCGAACAGGGCAATGTTTTTATCAACGGCAAACGCGCCGCCATTGGCGATCAGGTGGTCGCCGGGGATGTTGTTAAAG
TAAATGGTCGGTTGATTGAGCCGCGAGAAGCGGATGATTTAGTGCTGATTGCGCTGAATAAACCGGTTGGCATTGTCAGT
ACCACTGAAGACGGCGAACGCGACAATATTGTCGATTTTGTGAACCACAGCAAACGTATCTTCCCGATTGGTCGTCTGGA
TAAAGACTCGCAGGGGCTGATATTTCTGACTAACCACGGCGATCTGGTCAATAAGATTCTGCGTGCCGGTAACGATCACG
AAAAAGAGTATCTGGTGACGGTGGATAAACCGATTACCGACGAGTTTATTCGCGGTATGGGCGCTGGCGTGCCGATTCTG
GGTACCGTCACCAAAAAATGTAAGGTGAAAAAAGAGGCGCCGTTTGTCTTTCGCATTACCCTGGTGCAGGGATTAAACCG
TCAGATCCGCCGTATGTGTGAATACTTTGGTTATGAAGTGACGAAACTGGAGCGTACGCGCATTATGAACGTCAGCCTGA
GCGGGATTCCGCTGGGCGAGTGGCGCGATCTGACGGACGATGAGCTGATCGATCTGTTTAAGTTGATTGAGCGTTCCTCT
TCCGAAGCCAAACCAAAGGCCAAAGCGAAGCCGAAGACCACGGGTATTAAGCGACCGGTCGTTGCGATCGAAAAAAGCAA
TGAGAAAGCGCGCCCGGCATCAAGCGGTAAGCGCTTTACCTCGCCGGGACGAAAGAAGAAAGGACGTTAA

Upstream 100 bases:

>100_bases
GACGAAGATGATCCGTATGCCGATTTTAAAGTTCCTGACGATTTGATGTGGTAAGTCACATTCCCGACGTTTATGTAGAA
AAATTTCTCAGAGAGTAAAT

Downstream 100 bases:

>100_bases
CGTCTGCCGCGCGTCGGCGTATTCGCCGACCAGGAGAATGACGCTTCCGTATCGGGTTTATACGCCTGCTTTTTCTTCAA
CTGGCGGGCTTTTTTCGCTT

Product: 23S rRNA pseudouridine synthase F

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MLTDTSTRLNKYISESGICSRREADRFIEQGNVFINGKRAAIGDQVVAGDVVKVNGRLIEPREADDLVLIALNKPVGIVS
TTEDGERDNIVDFVNHSKRIFPIGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEYFGYEVTKLERTRIMNVSLSGIPLGEWRDLTDDELIDLFKLIERSS
SEAKPKAKAKPKTTGIKRPVVAIEKSNEKARPASSGKRFTSPGRKKKGR

Sequences:

>Translated_289_residues
MLTDTSTRLNKYISESGICSRREADRFIEQGNVFINGKRAAIGDQVVAGDVVKVNGRLIEPREADDLVLIALNKPVGIVS
TTEDGERDNIVDFVNHSKRIFPIGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEYFGYEVTKLERTRIMNVSLSGIPLGEWRDLTDDELIDLFKLIERSS
SEAKPKAKAKPKTTGIKRPVVAIEKSNEKARPASSGKRFTSPGRKKKGR
>Mature_289_residues
MLTDTSTRLNKYISESGICSRREADRFIEQGNVFINGKRAAIGDQVVAGDVVKVNGRLIEPREADDLVLIALNKPVGIVS
TTEDGERDNIVDFVNHSKRIFPIGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEYFGYEVTKLERTRIMNVSLSGIPLGEWRDLTDDELIDLFKLIERSS
SEAKPKAKAKPKTTGIKRPVVAIEKSNEKARPASSGKRFTSPGRKKKGR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=290, Percent_Identity=91.3793103448276, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI1787524, Length=271, Percent_Identity=31.3653136531365, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1788510, Length=232, Percent_Identity=29.7413793103448, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI87081838, Length=178, Percent_Identity=29.2134831460674, Blast_Score=78, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 32307; Mature: 32307

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTDTSTRLNKYISESGICSRREADRFIEQGNVFINGKRAAIGDQVVAGDVVKVNGRLIE
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCCCCCEEEECCEEEECCEEEC
PREADDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRIFPIGRLDKDSQGLIFLTNHG
CCCCCCEEEEEECCCCEEEECCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCEEEEECCC
DLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPILGTVTKKCKVKKEAPFVFRIT
HHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEH
LVQGLNRQIRRMCEYFGYEVTKLERTRIMNVSLSGIPLGEWRDLTDDELIDLFKLIERSS
HHHHCCHHHHHHHHHHCCEEEEEHEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCC
SEAKPKAKAKPKTTGIKRPVVAIEKSNEKARPASSGKRFTSPGRKKKGR
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLTDTSTRLNKYISESGICSRREADRFIEQGNVFINGKRAAIGDQVVAGDVVKVNGRLIE
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCCCCCEEEECCEEEECCEEEC
PREADDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRIFPIGRLDKDSQGLIFLTNHG
CCCCCCEEEEEECCCCEEEECCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCEEEEECCC
DLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPILGTVTKKCKVKKEAPFVFRIT
HHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEH
LVQGLNRQIRRMCEYFGYEVTKLERTRIMNVSLSGIPLGEWRDLTDDELIDLFKLIERSS
HHHHCCHHHHHHHHHHCCEEEEEHEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCC
SEAKPKAKAKPKTTGIKRPVVAIEKSNEKARPASSGKRFTSPGRKKKGR
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]