Click here to switch to the map view.

The map label for this gene is metB [C]

Identifier: 194735233

GI number: 194735233

Start: 3692645

End: 3692785

Strand: Direct

Name: metB [C]

Synonym: SeSA_A3822

Alternate gene names: 194735233

Gene position: 3692645-3692785 (Clockwise)

Preceding gene: 194737071

Following gene: 194735868

Centisome position: 78.42

GC content: 47.52

Gene sequence:

>141_bases
ATGACATATTCGCCTTTTGCGCCAGAGGAATGGCTAAAAGCAGGTATTACCGACGAACTTATCCGTCTTTCAGCGGGACC
TGAAGATCCCGACGATATTATTCATGACCTTGAACGCGCCAGCAGAAAAGCAGCATTCTGA

Upstream 100 bases:

>100_bases
GCATTTCTGAGGCTGGCAGTCAGCCGCTCAATTCTGTAAATTATGCCTCCTCGCGGCCAGTCCCGGTGATACCGAAACCC
TCGTTCAGCATCCTGCGTCT

Downstream 100 bases:

>100_bases
CGATTGCTCGGCGAACCGCTGTCACCGGTTTGCCGCATCGGGTATCAAGGCACTATAGCCTGGTTATAGGCAGCATTCTT
TATGAGAGAGCTGCGTATAT

Product: hypothetical protein

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: None

Number of amino acids: Translated: 46; Mature: 45

Protein sequence:

>46_residues
MTYSPFAPEEWLKAGITDELIRLSAGPEDPDDIIHDLERASRKAAF

Sequences:

>Translated_46_residues
MTYSPFAPEEWLKAGITDELIRLSAGPEDPDDIIHDLERASRKAAF
>Mature_45_residues
TYSPFAPEEWLKAGITDELIRLSAGPEDPDDIIHDLERASRKAAF

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 5174; Mature: 5043

Theoretical pI: Translated: 4.21; Mature: 4.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYSPFAPEEWLKAGITDELIRLSAGPEDPDDIIHDLERASRKAAF
CCCCCCCCHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TYSPFAPEEWLKAGITDELIRLSAGPEDPDDIIHDLERASRKAAF
CCCCCCCHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA