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The map label for this gene is hisF [H]

Identifier: 194735173

GI number: 194735173

Start: 2174647

End: 2175423

Strand: Direct

Name: hisF [H]

Synonym: SeSA_A2305

Alternate gene names: 194735173

Gene position: 2174647-2175423 (Clockwise)

Preceding gene: 194737880

Following gene: 194734563

Centisome position: 46.18

GC content: 54.95

Gene sequence:

>777_bases
ATGCTGGCAAAACGTATAATTCCGTGTCTGGATGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGA
GATCATTGGCGATATCGTTCCGCTGGCCAAACGCTATGCCGACGAAGGCGCGGACGAACTGGTGTTCTATGACATTACCG
CCTCCAGCGATGGCCGCGTAGTCGATAAAAGCTGGGTGGCGCGCGTTGCCGAGGTGATCGACATTCCGTTTTGTGTAGCG
GGCGGTATCCGGTCAATTGACGACGCCGCCAAAATTCTTTCTTTCGGGGCGGATAAGATCTCTATCAACTCCCCAGCGCT
GGCTGACCCAACGCTGATTACCCGTCTGGCTGACCGTTTTGGCGTACAGTGCATTGTCGTCGGGATTGATACCTGGTTTG
ACGACGCCACGGGGAAATATCATGTTAACCAGTATACCGGCGATGAAAACCGTACCCGCGTGACGCAGTGGGAGACGCTG
GATTGGGTGCAAGTGGTACAACAGCGCGGCGCGGGGGAAATCGTCCTGAATATGATGAACCAGGACGGCGTGCGTAACGG
TTATGATCTGACGCAGTTGAAAAAAGTCCGTGACGTTTGCCGCGTGCCGCTGATCGCCTCCGGCGGCGCGGGCACGATGG
AACACTTTCTTGAGGCATTCCGTGATGCCGATGTCGACGGCGCGCTTGCCGCCTCCGTTTTTCACAAGCAAATCATCAAT
ATTGGCGAATTAAAAGCGTACCTGGCAGGCCAGGGCGTGGAGATCAGGATATGTTAA

Upstream 100 bases:

>100_bases
ATATCGATGATATTGCCGCCCTGCGCGGCACCGGCGTGCGCGGCGTGATTGTCGGACGCGCGCTGTTGGAAGGGAAATTT
ACCGTTAAGGAGGCCATCCA

Downstream 100 bases:

>100_bases
CAGAGCAACAACGCCGCGAGCTGGACTGGGAAAAAACCGATGGCCTGATGCCAGCCATCGTGCAACATGCAGTATCCGGC
GAAGTATTGATGCTGGGCTA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYADEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIRSIDDAAKILSFGADKISINSPALADPTLITRLADRFGVQCIVVGIDTWFDDATGKYHVNQYTGDENRTRVTQWETL
DWVQVVQQRGAGEIVLNMMNQDGVRNGYDLTQLKKVRDVCRVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
IGELKAYLAGQGVEIRIC

Sequences:

>Translated_258_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYADEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIRSIDDAAKILSFGADKISINSPALADPTLITRLADRFGVQCIVVGIDTWFDDATGKYHVNQYTGDENRTRVTQWETL
DWVQVVQQRGAGEIVLNMMNQDGVRNGYDLTQLKKVRDVCRVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
IGELKAYLAGQGVEIRIC
>Mature_258_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYADEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIRSIDDAAKILSFGADKISINSPALADPTLITRLADRFGVQCIVVGIDTWFDDATGKYHVNQYTGDENRTRVTQWETL
DWVQVVQQRGAGEIVLNMMNQDGVRNGYDLTQLKKVRDVCRVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
IGELKAYLAGQGVEIRIC

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=94.1860465116279, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI87082028, Length=236, Percent_Identity=25, Blast_Score=66, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319725, Length=320, Percent_Identity=30, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 28339; Mature: 28339

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYADEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCE
VDKSWVARVAEVIDIPFCVAGGIRSIDDAAKILSFGADKISINSPALADPTLITRLADRF
ECHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDTWFDDATGKYHVNQYTGDENRTRVTQWETLDWVQVVQQRGAGEIVLNMMN
CCEEEEEEEECCCCCCCCEEEEEEECCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEECC
QDGVRNGYDLTQLKKVRDVCRVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IGELKAYLAGQGVEIRIC
HHHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYADEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCE
VDKSWVARVAEVIDIPFCVAGGIRSIDDAAKILSFGADKISINSPALADPTLITRLADRF
ECHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDTWFDDATGKYHVNQYTGDENRTRVTQWETLDWVQVVQQRGAGEIVLNMMN
CCEEEEEEEECCCCCCCCEEEEEEECCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEECC
QDGVRNGYDLTQLKKVRDVCRVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IGELKAYLAGQGVEIRIC
HHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA