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The map label for this gene is wcaF [H]

Identifier: 194735057

GI number: 194735057

Start: 2215292

End: 2215846

Strand: Reverse

Name: wcaF [H]

Synonym: SeSA_A2343

Alternate gene names: 194735057

Gene position: 2215846-2215292 (Counterclockwise)

Preceding gene: 194734994

Following gene: 194738147

Centisome position: 47.05

GC content: 51.17

Gene sequence:

>555_bases
ATGCAGGATCTTAGCGGCTTTACGGTGCCGAAGGGGTTCCGCGGCGCTAATGCGCTAAAAGTACAATTATGGTGGGCGGT
ACAGGCGACATTATTCGCCGGGTCGCCGCAAATATTGTATCGCTGGCGAGCCTTTTTGCTGCGTCTGTTTGGCGCCAAAA
TTGGAAAGAATGTGGTTATTCGACCGTCAGTAAAAATTACCTATCCGTGGAAATTAACCGTCGGCGATTATGCCTGGGTA
GGCGACGACGCGGTGTTATATACGTTGGGTGAAATTAATATTGGCGCACATGCGGTCATTTCACAAAAAGGGTATTTGTG
TACCGGTAGCCATGATTATACCAGCGCCCATTTCGATATTAACGCCGCGCCGATTGTTATTGGCGAAAAATGTTGGCTGG
CAACCGATGTTTTTGTCGCGCCCGGCGTGATGATAGGTCATGGCACCGTCGTCGGCGCGCGCAGCAGCGTATTTAAATCA
TTACCGGCAAACGCGATTTGTCGGGGCAATCCCGCAGTGGTAACGCGCCAGCGCGTTCAGAAAGTTACTCCCTAA

Upstream 100 bases:

>100_bases
TGTGCCAGGGTTTTGGGCGCAATTATCCTATTTATTACGTCTGAAAACGACGGGTAAAACAAAAGCGCGATATAACAAAG
TCTAATCACCGGGAGTTATT

Downstream 100 bases:

>100_bases
CGGGACTATTTGAGGAAATGAAAATGTCAAAAGTCGCTCTCATTACTGGCGTAACCGGACAGGATGGTTCTTACCTGGCA
GAATTTCTGCTGGAAAAAGG

Product: putative colanic acid biosynthesis acetyltransferase WcaF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MQDLSGFTVPKGFRGANALKVQLWWAVQATLFAGSPQILYRWRAFLLRLFGAKIGKNVVIRPSVKITYPWKLTVGDYAWV
GDDAVLYTLGEINIGAHAVISQKGYLCTGSHDYTSAHFDINAAPIVIGEKCWLATDVFVAPGVMIGHGTVVGARSSVFKS
LPANAICRGNPAVVTRQRVQKVTP

Sequences:

>Translated_184_residues
MQDLSGFTVPKGFRGANALKVQLWWAVQATLFAGSPQILYRWRAFLLRLFGAKIGKNVVIRPSVKITYPWKLTVGDYAWV
GDDAVLYTLGEINIGAHAVISQKGYLCTGSHDYTSAHFDINAAPIVIGEKCWLATDVFVAPGVMIGHGTVVGARSSVFKS
LPANAICRGNPAVVTRQRVQKVTP
>Mature_184_residues
MQDLSGFTVPKGFRGANALKVQLWWAVQATLFAGSPQILYRWRAFLLRLFGAKIGKNVVIRPSVKITYPWKLTVGDYAWV
GDDAVLYTLGEINIGAHAVISQKGYLCTGSHDYTSAHFDINAAPIVIGEKCWLATDVFVAPGVMIGHGTVVGARSSVFKS
LPANAICRGNPAVVTRQRVQKVTP

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1788367, Length=180, Percent_Identity=86.6666666666667, Blast_Score=328, Evalue=2e-91,
Organism=Escherichia coli, GI1786537, Length=138, Percent_Identity=28.2608695652174, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.- [C]

Molecular weight: Translated: 19987; Mature: 19987

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDLSGFTVPKGFRGANALKVQLWWAVQATLFAGSPQILYRWRAFLLRLFGAKIGKNVVI
CCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEE
RPSVKITYPWKLTVGDYAWVGDDAVLYTLGEINIGAHAVISQKGYLCTGSHDYTSAHFDI
CCCEEEEEEEEEEECCEEEECCCEEEEEEECCCCCCEEEEECCCEEEECCCCCEEEEEEC
NAAPIVIGEKCWLATDVFVAPGVMIGHGTVVGARSSVFKSLPANAICRGNPAVVTRQRVQ
CCCCEEEECCEEEEEEHHHCCCEEEECCEEEECHHHHHHHCCCCEEECCCCCEEEHHHHH
KVTP
CCCC
>Mature Secondary Structure
MQDLSGFTVPKGFRGANALKVQLWWAVQATLFAGSPQILYRWRAFLLRLFGAKIGKNVVI
CCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEE
RPSVKITYPWKLTVGDYAWVGDDAVLYTLGEINIGAHAVISQKGYLCTGSHDYTSAHFDI
CCCEEEEEEEEEEECCEEEECCCEEEEEEECCCCCCEEEEECCCEEEECCCCCEEEEEEC
NAAPIVIGEKCWLATDVFVAPGVMIGHGTVVGARSSVFKSLPANAICRGNPAVVTRQRVQ
CCCCEEEECCEEEEEEHHHCCCEEEECCEEEECHHHHHHHCCCCEEECCCCCEEEHHHHH
KVTP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]