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The map label for this gene is potB

Identifier: 194734807

GI number: 194734807

Start: 1275828

End: 1276691

Strand: Reverse

Name: potB

Synonym: SeSA_A1304

Alternate gene names: 194734807

Gene position: 1276691-1275828 (Counterclockwise)

Preceding gene: 194736998

Following gene: 194734735

Centisome position: 27.11

GC content: 49.31

Gene sequence:

>864_bases
ATGAAGAACACAAGTAAATTCCAGAATGTAGTGATTGTCACTATCGTCGGTTGGCTTGTGTTGTTTGTTTTTCTGCCCAA
CCTGATGATCATTGGCACCAGCTTTCTGACCCGTGACGACGCCAGTTTCGTTAAGATGGTCTTTACGCTGGATAACTACG
CACGCCTGCTCGATCCGCTCTATTTTGAAGTGCTCCTGCACTCTCTGAATATGGCGCTGATCGCCACCCTCTCCTGCCTG
GTGCTGGGCTATCCTTTTGCCTGGTTTCTGGCGAAGTTACCGGAAAAGATACGTCCGTTATTGCTGTTTTTGCTGATTGT
TCCTTTCTGGACCAACTCACTGATCCGCATCTATGGGTTGAAAATATTCCTGAGCACTAAAGGTTATCTCAATGAATTCC
TGCTGTGGCTGGGCGTGATTGATACGCCGATTCGCATCATGTTCACCCCGAGCGCCGTTATTATCGGCCTGGTTTACATT
TTGCTGCCGTTTATGGTCATGCCGCTCTACTCCAGCATTGAGAAGCTCGACAAACCACTACTGGAGGCCGCACGCGATCT
CGGCGCCAGTAAAATGCAAACGTTCATCCGCATCATTATTCCATTAACCATGCCGGGCATTGTCGCCGGTTGCCTGCTCG
TGATGCTACCGGCAATGGGCCTGTTCTACGTCTCCGATTTAATGGGTGGCGCGAAAAACCTGCTGATTGGTAACGTCATT
AAGGTACAGTTCCTGAATATTCGCGACTGGCCGTTTGGCGCGGCTACCAGCATTACGCTGACCATTGTGATGGGACTCAT
GCTGTTGATTTACTGGCGGGCTTCCCGCTTATTGAATAAGAAGGTGAGTGATATAAGCGATTAA

Upstream 100 bases:

>100_bases
GTCAGCGAATTCTTCAACGAAGACGATCCTGACTTCGACCATTCACTTGACCAAAAAATGGCCATTAGTTGGGTAGAAAG
CTGGGAGGTCGTACTGGCTG

Downstream 100 bases:

>100_bases
TTGCGCAACGCTAACAAATCCATACGCATTCAGGCATGAAGTTTATTCAAGGGTAAACTTCATGCCTCCGGCATAAAAAA
CGCATGAAAGCAGTTGCCGC

Product: spermidine/putrescine ABC transporter membrane protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFVKMVFTLDNYARLLDPLYFEVLLHSLNMALIATLSCL
VLGYPFAWFLAKLPEKIRPLLLFLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
LLPFMVMPLYSSIEKLDKPLLEAARDLGASKMQTFIRIIIPLTMPGIVAGCLLVMLPAMGLFYVSDLMGGAKNLLIGNVI
KVQFLNIRDWPFGAATSITLTIVMGLMLLIYWRASRLLNKKVSDISD

Sequences:

>Translated_287_residues
MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFVKMVFTLDNYARLLDPLYFEVLLHSLNMALIATLSCL
VLGYPFAWFLAKLPEKIRPLLLFLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
LLPFMVMPLYSSIEKLDKPLLEAARDLGASKMQTFIRIIIPLTMPGIVAGCLLVMLPAMGLFYVSDLMGGAKNLLIGNVI
KVQFLNIRDWPFGAATSITLTIVMGLMLLIYWRASRLLNKKVSDISD
>Mature_287_residues
MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFVKMVFTLDNYARLLDPLYFEVLLHSLNMALIATLSCL
VLGYPFAWFLAKLPEKIRPLLLFLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
LLPFMVMPLYSSIEKLDKPLLEAARDLGASKMQTFIRIIIPLTMPGIVAGCLLVMLPAMGLFYVSDLMGGAKNLLIGNVI
KVQFLNIRDWPFGAATSITLTIVMGLMLLIYWRASRLLNKKVSDISD

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1787369, Length=273, Percent_Identity=96.7032967032967, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI1787080, Length=215, Percent_Identity=46.9767441860465, Blast_Score=201, Evalue=6e-53,
Organism=Escherichia coli, GI1787713, Length=266, Percent_Identity=29.3233082706767, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788764, Length=198, Percent_Identity=26.7676767676768, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786980, Length=185, Percent_Identity=30.2702702702703, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): POTB_SALTI (P0A2J8)

Other databases:

- EMBL:   AL627269
- EMBL:   AE014613
- RefSeq:   NP_455717.1
- RefSeq:   NP_805471.1
- ProteinModelPortal:   P0A2J8
- GeneID:   1071313
- GeneID:   1247668
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t1695
- KEGG:   sty:STY1265
- HOGENOM:   HBG685300
- OMA:   MIIATSF
- ProtClustDB:   PRK09497
- BioCyc:   SENT209261:T1695-MONOMER
- BioCyc:   SENT220341:STY1265-MONOMER
- InterPro:   IPR000515

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 32365; Mature: 32365

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1e4394e0)-; HASH(0x1b93c204)-; HASH(0x1e4cdb5c)-; HASH(0x1e341534)-; HASH(0x1e1ec4b4)-; HASH(0x1e53c768)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
>Mature Secondary Structure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EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504