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The map label for this gene is dacD [H]

Identifier: 194734763

GI number: 194734763

Start: 2115750

End: 2116922

Strand: Reverse

Name: dacD [H]

Synonym: SeSA_A2232

Alternate gene names: 194734763

Gene position: 2116922-2115750 (Counterclockwise)

Preceding gene: 194735263

Following gene: 194734276

Centisome position: 44.95

GC content: 52.34

Gene sequence:

>1173_bases
ATGCTGTTGAAACGCCGTTTGTTTATTGCCGCTTCTTTATTCGCAATGCACCTTTCTCCTGCGCTTGCGGCCGATGCCGT
AAGTTTTGCTCCCCAACCGCCAGCCATTGATGCAGGCGCCTGGGTGTTGATGGATTACACTACGGGACAGGTTCTTACCG
CCGGTAATGAACACCAGCAACGTAATCCCGCCAGCCTTACCAAGCTAATGACGGGCTACGTCGTCGATCGCGCCATCGAT
AGTCATCGTATCAGCCCTGACGATATCGTCACCGTTGGGCGCGACGCCTGGGCGAAGGATAATCCGGTGTTTGTCGGCTC
CTCGTTGATGTTTTTAAAAGAAGGGGATCGCGTGTCGGTACGCGATTTAAGCCGTGGCTTAATTGTGGATTCCGGTAACG
ATGCCTGTGTGGCGCTGGCGGATTATATCGCGGGCGGGCAGCCGCAGTTTGTGGCGATGATGAACAGCTATGTGAAAAAA
CTCAATTTACAGGATACCCATTTTGAAACCGTCCACGGTCTGGATGCGCCGGGACAACATAGCTCCGCGTATGACCTGGC
TGTACTCTCCCGGGCGATTATTCACGGCGAACCTGAATTTTATCACATGTACAGCGAGAAAAGCCTGACCTGGAACGGCA
TCACGCAGCAGAACCGCAACGGCTTGTTATGGGATAAGACCATGCATATTGATGGGCTTAAAACCGGACACACATCGGGG
GCGGGATTTAATCTTATCGCATCGGCGGTGGACGGGCAGCGTCGACTTATCGCTGTTGTGATGGGAGCGAAAAGTTCGAA
AGGCCGTGAAGAACAGGCGCGAAAACTGTTGCAGTGGGGCCTGCAAAACTTTGCGACGGTGCAGATTTTGCACAGCGGTA
AAAAAGTGGGCAGCGAGCGAATCTGGTATGGAGACAAAGAAAAGATTGCGCTGGGTACGGAACAGGATTTCTGGATGGCG
CTGCCTAAAGCGGAAATTCCGCATATCAAAGCAAAATATGTGCTGGATAGAAAAGAGCTGGAGGCGCCAATCGCCGCTCA
TCAACAGGTAGGTGAAATAGAGCTCTACGATCGGGATAAACTGATCGCTCAGTGGCCGCTGGTCACTCTGGAATCGGTGG
GTAAAGGCGGTATGTTCTCTCGTCTGAGCGACTATTTTCAGCATAAAGCGTAA

Upstream 100 bases:

>100_bases
CAAGAAAATGTGCCTTGTGTCACATTTTCTTGCAATTTATCCATGTTTGTTTAACTGAACTTCCGTAAAAAGAACGGCAA
ATAGAGACCATCCTGAGGAC

Downstream 100 bases:

>100_bases
GCCGTTGTCACATACCGTTCACCGTCATCTGAGGTGTCAAAAGGGCGGGATTGCGAGGTTGCTCACAAAATGATCACGCT
GTCCTTGTGACCGTAAAATG

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6B; PBP-6B [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MLLKRRLFIAASLFAMHLSPALAADAVSFAPQPPAIDAGAWVLMDYTTGQVLTAGNEHQQRNPASLTKLMTGYVVDRAID
SHRISPDDIVTVGRDAWAKDNPVFVGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQPQFVAMMNSYVKK
LNLQDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRNGLLWDKTMHIDGLKTGHTSG
AGFNLIASAVDGQRRLIAVVMGAKSSKGREEQARKLLQWGLQNFATVQILHSGKKVGSERIWYGDKEKIALGTEQDFWMA
LPKAEIPHIKAKYVLDRKELEAPIAAHQQVGEIELYDRDKLIAQWPLVTLESVGKGGMFSRLSDYFQHKA

Sequences:

>Translated_390_residues
MLLKRRLFIAASLFAMHLSPALAADAVSFAPQPPAIDAGAWVLMDYTTGQVLTAGNEHQQRNPASLTKLMTGYVVDRAID
SHRISPDDIVTVGRDAWAKDNPVFVGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQPQFVAMMNSYVKK
LNLQDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRNGLLWDKTMHIDGLKTGHTSG
AGFNLIASAVDGQRRLIAVVMGAKSSKGREEQARKLLQWGLQNFATVQILHSGKKVGSERIWYGDKEKIALGTEQDFWMA
LPKAEIPHIKAKYVLDRKELEAPIAAHQQVGEIELYDRDKLIAQWPLVTLESVGKGGMFSRLSDYFQHKA
>Mature_390_residues
MLLKRRLFIAASLFAMHLSPALAADAVSFAPQPPAIDAGAWVLMDYTTGQVLTAGNEHQQRNPASLTKLMTGYVVDRAID
SHRISPDDIVTVGRDAWAKDNPVFVGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQPQFVAMMNSYVKK
LNLQDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRNGLLWDKTMHIDGLKTGHTSG
AGFNLIASAVDGQRRLIAVVMGAKSSKGREEQARKLLQWGLQNFATVQILHSGKKVGSERIWYGDKEKIALGTEQDFWMA
LPKAEIPHIKAKYVLDRKELEAPIAAHQQVGEIELYDRDKLIAQWPLVTLESVGKGGMFSRLSDYFQHKA

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Note=N-terminal lies in the periplasmic space (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=388, Percent_Identity=87.1134020618557, Blast_Score=713, Evalue=0.0,
Organism=Escherichia coli, GI1786851, Length=387, Percent_Identity=48.062015503876, Blast_Score=390, Evalue=1e-110,
Organism=Escherichia coli, GI1787062, Length=385, Percent_Identity=47.5324675324675, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI87082054, Length=274, Percent_Identity=26.6423357664234, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43083; Mature: 43083

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKRRLFIAASLFAMHLSPALAADAVSFAPQPPAIDAGAWVLMDYTTGQVLTAGNEHQQ
CCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEECCCCCCC
RNPASLTKLMTGYVVDRAIDSHRISPDDIVTVGRDAWAKDNPVFVGSSLMFLKEGDRVSV
CCHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCCCCCCCEEECCEEEEEECCCEEEH
RDLSRGLIVDSGNDACVALADYIAGGQPQFVAMMNSYVKKLNLQDTHFETVHGLDAPGQH
HHCCCCEEEECCCCCEEEEEHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCC
SSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRNGLLWDKTMHIDGLKTGHTSG
CCHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCCCCCEEEEEEEECCCCCCCCCC
AGFNLIASAVDGQRRLIAVVMGAKSSKGREEQARKLLQWGLQNFATVQILHSGKKVGSER
CCHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCHHCCCCC
IWYGDKEKIALGTEQDFWMALPKAEIPHIKAKYVLDRKELEAPIAAHQQVGEIELYDRDK
EEECCCCEEEECCCCCCEEECCCCCCCCCCHHEEECHHHHCCCHHHHHCCCCEEEECCCC
LIAQWPLVTLESVGKGGMFSRLSDYFQHKA
EEECCCCHHHHHCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLLKRRLFIAASLFAMHLSPALAADAVSFAPQPPAIDAGAWVLMDYTTGQVLTAGNEHQQ
CCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEECCCCCCC
RNPASLTKLMTGYVVDRAIDSHRISPDDIVTVGRDAWAKDNPVFVGSSLMFLKEGDRVSV
CCHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCCCCCCCEEECCEEEEEECCCEEEH
RDLSRGLIVDSGNDACVALADYIAGGQPQFVAMMNSYVKKLNLQDTHFETVHGLDAPGQH
HHCCCCEEEECCCCCEEEEEHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCC
SSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRNGLLWDKTMHIDGLKTGHTSG
CCHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCCCCCEEEEEEEECCCCCCCCCC
AGFNLIASAVDGQRRLIAVVMGAKSSKGREEQARKLLQWGLQNFATVQILHSGKKVGSER
CCHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCHHCCCCC
IWYGDKEKIALGTEQDFWMALPKAEIPHIKAKYVLDRKELEAPIAAHQQVGEIELYDRDK
EEECCCCEEEECCCCCCEEECCCCCCCCCCHHEEECHHHHCCCHHHHHCCCCEEEECCCC
LIAQWPLVTLESVGKGGMFSRLSDYFQHKA
EEECCCCHHHHHCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7737516; 11677609; 7751291 [H]