Click here to switch to the map view.

The map label for this gene is thiC [H]

Identifier: 194734581

GI number: 194734581

Start: 4246493

End: 4248388

Strand: Reverse

Name: thiC [H]

Synonym: SeSA_A4374

Alternate gene names: 194734581

Gene position: 4248388-4246493 (Counterclockwise)

Preceding gene: 194736012

Following gene: 194733947

Centisome position: 90.22

GC content: 58.65

Gene sequence:

>1896_bases
ATGTCTACAACAACGTTAACCCGCCGCGAGCAGCGCGCTAAAGCCCAGCATTTTATCGATACGCTGGAAGGCACCGCATT
TCCCAACTCGAAACGCATCTACGTGACCGGTTCGCAGCATGATATTCGCGTACCGATGCGCGAAATTCAACTTAGCCCAA
CGCTCATCGGCGGCAGCAAAGACAACCCGCAGTTTGAAGAGAACGAAGCCGTGCCGGTGTACGACACCTCCGGCCCCTAT
GGCGATCCTGAGGTGGCGATTAACGTCCAGCAGGGTCTGGCGAAACTGCGCCAGCCATGGATTGACGCACGTAACGATAG
CGAAGAATTAGACGACCGTAGCTCGGCTTATACCAGAGAACGTCTGGCCGACGATGGCCTGGACGATCTGCGTTTTACCG
GCCTGCTGACGCCAAAACGCGCTAAAGCGGGCAAGCGCGTCACCCAGTTACACTACGCCCGCAACGGGATCGTCACTCCC
GAAATGGAGTTCATCGCCATCCGTGAAAATATGGGCCGCGAGCGCATTCGCAGTGAAGTACTGCGCCACCAGCATCCGGG
GATGAGCTTTGGCGCACGCCTGCCGGAAAACATCACCCCGGAATTCGTGCGTGATGAAGTCGCCGCCGGACGCGCCATCA
TCCCCGCCAACATCAACCACCCGGAATCGGAGCCGATGATTATTGGCCGCAACTTCCTGGTGAAGGTCAACGCCAATATC
GGCAACTCGGCGGTGACCTCCTCTATCGAAGAAGAGGTGGAAAAACTGGTGTGGGCGACGCGCTGGGGGGCGGATACGGT
AATGGACCTTTCCACCGGGCGCTATATTCACGAAACCCGTGAGTGGATCCTGCGTAATAGCCCAGTACCAATCGGCACGG
TGCCGATCTACCAGGCGCTGGAAAAGGTCAACGGGATCGCCGAGGATCTTACCTGGGAAGCCTTCCGCGACACGCTGCTG
GAGCAGGCCGAACAGGGCGTCGACTACTTCACCATTCACGCGGGCGTGCTGCTGCGTTACGTGCCGATGACCGCCAAGCG
CCTGACCGGCATTGTCTCGCGCGGCGGTTCGATCATGGCGAAGTGGTGTCTCTCCCATCACAAAGAGAACTTCCTGTTCG
AACATTTCCGCGAGATTTGCGAAATCTGCGCCGCCTACGACGTTTCCCTGTCGCTGGGCGACGGCCTGCGACCGGGATCG
ATTCAGGACGCGAACGACGAAGCGCAGTTCTCCGAGCTGCATACGCTGGGCGAGCTGACCAAAATCGCCTGGGAATACGA
CGTGCAGGTGATGATTGAAGGCCCGGGCCACGTGCCGATGCATATGATTCAGCGCAACATGACCGAAGAGCTGGAGCACT
GCCATGAAGCGCCGTTCTACACCTTAGGGCCGCTGACTACCGATATCGCGCCGGGCTATGACCACTTCACCTCCGGCATT
GGCGCGGCGATGATCGGCTGGTTCGGCTGTGCGATGCTGTGTTACGTGACGCCGAAAGAGCATCTCGGCCTGCCGAACAA
AGAAGATGTGAAGCAGGGGCTGATCACCTACAAGATCGCCGCCCACGCCGCTGACTTAGCCAAAGGGCATCCGGGCGCGC
AGATTCGCGATAACGCGATGTCGAAAGCGCGCTTTGAATTCCGCTGGGAGGATCAGTTTAACCTCGCGCTCGACCCGTTC
ACCGCCCGCGCCTGGCACGATGAAACCCTGCCACAGGAGTCCGGCAAAGTGGCGCACTTTTGCTCGATGTGCGGGCCGAA
ATTCTGCTCAATGAAAATCAGCCAGGAAGTTCGTGATTACGCCGCCGCGCAAACTATTGAAGTCGGGATGGCGGATATGT
CGGAAAACTTCCGCGCCAAAGGCGGCGAAATCTATCTCAAGCGGGAGGAAGTCTGA

Upstream 100 bases:

>100_bases
AGAGTAATTCTGCTTTATGCTGCTGCCTTTACGGGCGGCGCACATCCATTACTCCATCCGTCGTCTGACAAGCCACGTCC
TTAATTTTTTGGAATGAGCT

Downstream 100 bases:

>100_bases
TGTATCAGCCTGATTTCCCGACCGTACCGTTTCGCCTCGGGCTTTACCCGGTGGTGGACAGCGTGCAATGGATTGAACGC
CTGCTGGAGGCGGGCGTGCG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 631; Mature: 630

Protein sequence:

>631_residues
MSTTTLTRREQRAKAQHFIDTLEGTAFPNSKRIYVTGSQHDIRVPMREIQLSPTLIGGSKDNPQFEENEAVPVYDTSGPY
GDPEVAINVQQGLAKLRQPWIDARNDSEELDDRSSAYTRERLADDGLDDLRFTGLLTPKRAKAGKRVTQLHYARNGIVTP
EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITPEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANI
GNSAVTSSIEEEVEKLVWATRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLL
EQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHKENFLFEHFREICEICAAYDVSLSLGDGLRPGS
IQDANDEAQFSELHTLGELTKIAWEYDVQVMIEGPGHVPMHMIQRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTSGI
GAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPF
TARAWHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRAKGGEIYLKREEV

Sequences:

>Translated_631_residues
MSTTTLTRREQRAKAQHFIDTLEGTAFPNSKRIYVTGSQHDIRVPMREIQLSPTLIGGSKDNPQFEENEAVPVYDTSGPY
GDPEVAINVQQGLAKLRQPWIDARNDSEELDDRSSAYTRERLADDGLDDLRFTGLLTPKRAKAGKRVTQLHYARNGIVTP
EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITPEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANI
GNSAVTSSIEEEVEKLVWATRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLL
EQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHKENFLFEHFREICEICAAYDVSLSLGDGLRPGS
IQDANDEAQFSELHTLGELTKIAWEYDVQVMIEGPGHVPMHMIQRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTSGI
GAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPF
TARAWHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRAKGGEIYLKREEV
>Mature_630_residues
STTTLTRREQRAKAQHFIDTLEGTAFPNSKRIYVTGSQHDIRVPMREIQLSPTLIGGSKDNPQFEENEAVPVYDTSGPYG
DPEVAINVQQGLAKLRQPWIDARNDSEELDDRSSAYTRERLADDGLDDLRFTGLLTPKRAKAGKRVTQLHYARNGIVTPE
MEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITPEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANIG
NSAVTSSIEEEVEKLVWATRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLE
QAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHKENFLFEHFREICEICAAYDVSLSLGDGLRPGSI
QDANDEAQFSELHTLGELTKIAWEYDVQVMIEGPGHVPMHMIQRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTSGIG
AAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFT
ARAWHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRAKGGEIYLKREEV

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=630, Percent_Identity=93.1746031746032, Blast_Score=1247, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 70875; Mature: 70744

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTTLTRREQRAKAQHFIDTLEGTAFPNSKRIYVTGSQHDIRVPMREIQLSPTLIGGSK
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEECCHHHHEECCEEECCCC
DNPQFEENEAVPVYDTSGPYGDPEVAINVQQGLAKLRQPWIDARNDSEELDDRSSAYTRE
CCCCCCCCCCCEEEECCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RLADDGLDDLRFTGLLTPKRAKAGKRVTQLHYARNGIVTPEMEFIAIRENMGRERIRSEV
HHHCCCCCHHEEEECCCCHHHHHCCHHHHHHHHHCCCCCCCHHEEEEHHHCCHHHHHHHH
LRHQHPGMSFGARLPENITPEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANI
HHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEEECCC
GNSAVTSSIEEEVEKLVWATRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQAL
CCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHH
EKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHH
KWCLSHHKENFLFEHFREICEICAAYDVSLSLGDGLRPGSIQDANDEAQFSELHTLGELT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KIAWEYDVQVMIEGPGHVPMHMIQRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTSGI
HHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHH
GAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAM
HHHHHHHHHHHHHHEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
SKARFEFRWEDQFNLALDPFTARAWHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDY
HHCEEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
AAAQTIEVGMADMSENFRAKGGEIYLKREEV
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
>Mature Secondary Structure 
STTTLTRREQRAKAQHFIDTLEGTAFPNSKRIYVTGSQHDIRVPMREIQLSPTLIGGSK
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEECCHHHHEECCEEECCCC
DNPQFEENEAVPVYDTSGPYGDPEVAINVQQGLAKLRQPWIDARNDSEELDDRSSAYTRE
CCCCCCCCCCCEEEECCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RLADDGLDDLRFTGLLTPKRAKAGKRVTQLHYARNGIVTPEMEFIAIRENMGRERIRSEV
HHHCCCCCHHEEEECCCCHHHHHCCHHHHHHHHHCCCCCCCHHEEEEHHHCCHHHHHHHH
LRHQHPGMSFGARLPENITPEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANI
HHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEEECCC
GNSAVTSSIEEEVEKLVWATRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQAL
CCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHH
EKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHH
KWCLSHHKENFLFEHFREICEICAAYDVSLSLGDGLRPGSIQDANDEAQFSELHTLGELT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KIAWEYDVQVMIEGPGHVPMHMIQRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTSGI
HHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHH
GAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAM
HHHHHHHHHHHHHHEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
SKARFEFRWEDQFNLALDPFTARAWHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDY
HHCEEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
AAAQTIEVGMADMSENFRAKGGEIYLKREEV
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA