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The map label for this gene is folC [H]

Identifier: 194734517

GI number: 194734517

Start: 2480452

End: 2481720

Strand: Reverse

Name: folC [H]

Synonym: SeSA_A2595

Alternate gene names: 194734517

Gene position: 2481720-2480452 (Counterclockwise)

Preceding gene: 194734039

Following gene: 194734609

Centisome position: 52.7

GC content: 58.47

Gene sequence:

>1269_bases
ATGAAAAACAAATCTATACCTCAAGCTGCGTCGCCCCTGGCCTCGTGGCTTTCTTATCTGGAAAACCTGCACAGCAAGAG
TATCGATTTAGGCCTTGAGCGCGTAAGCCAGGTGGCAGCGCGTCTGGACATCCTCAAGCCTGCGCCGTTCGTTTTTACCG
TTGCGGGAACCAACGGCAAAGGTACGACCTGTCGCACGCTGGAGTCGGTGTTAATCGCGGCGGGATACCGTGTGGGCGTC
TATAGTTCGCCTCATCTGGTACGTTACACCGAGCGCGTGCGCGTGCAGGGGAAAGAATTAGCGGAATCCGCGCATACCGC
GTCCTTTGCGGAAATAGAAACGGCGCGCGGCGATATTTCATTGACCTATTTTGAATATGGTACGTTGTCGGCATTGTGGC
TGTTTAAACAGGCTAACCTTGACGTGGTGATTCTGGAGGTCGGGTTGGGCGGGCGCCTGGACGCCACGAACATTGTGGAT
GCCGATGTAGCGGTAATTACCAGCATCGCGCTTGACCATACTGACTGGCTGGGGCCGGATCGCGAAAGTATCGGCCGCGA
GAAAGCCGGGATTTTTCGCGCGGAAAAACCCGCTATTGTCGGCGAACCGGAAATGCCGGCAACTATCGCGGATGTCGCGC
AGGAGACCGGCGCGCTGTTGCGGCGTCGCGGCGTTGACTGGCGTTATGAGGTGACAGCGACCCACTGGGCGTTTACCGAT
GGCGACGGTACGCTTGCCGGACTGCCGTTGCCGCAGGTTCCCCAGCCGAATGCCGCCACCGCCCTGGCCGCGCTGCGCGC
CAGCAGGTTGAACATTGACGAGCAGGCGATTCGCGACGGTATTGCGCAGGCTACCTTGCCGGGACGTTTTCAAATCGTGA
GTGAGTCGCCGCGCGTTATCTTTGATGTCGCCCATAATCCCCACGCGGCGGAATACCTGACGGGTCGTCTGAAAATGTTG
CCGAAACGCGGGCGCGTGCTTGCGGTGATTGGTATGCTGCATGATAAAGATATCGCCGGCACGTTAGCCTGGTTGAAAAG
CGTGGTTGACGACTGGTATTGTGCTCCGTTAGAGGGGCCGCGCGGCGCGACGGCGGAACAACTTCTGGAACATCTGGGGA
AAGGGAATGTCTATGATAGCGTCGCGCAGGCATGGCAGGCCGCTATCGACGCGGCGCAGCCAGAAGATACCGTGCTGGTG
TGCGGATCGTTCCACACCGTCGCACATGTTATGGAAGTGATAGACGCGGGGAGAATCGGTGGCGAGTAA

Upstream 100 bases:

>100_bases
CCTCCGGCGCCGGATCAGGAGTCTGAGGCCTGATAACTGAAAAGGGCAGGGCCGCAGGCTCTGCCCTTTTGCTTTTCACC
GTAAAGAAGCAGCGGAATCA

Downstream 100 bases:

>100_bases
GTTTCAGAATCGTTTAGTCGGCACGATTGTGTTAGTCGCGCTGGGGGTTATTGTGCTTCCCGGTCTGCTGGACGGACAGA
AAAAGCATTATCAGGATGAA

Product: bifunctional folylpolyglutamate synthase/ dihydrofolate synthase

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKNKSIPQAASPLASWLSYLENLHSKSIDLGLERVSQVAARLDILKPAPFVFTVAGTNGKGTTCRTLESVLIAAGYRVGV
YSSPHLVRYTERVRVQGKELAESAHTASFAEIETARGDISLTYFEYGTLSALWLFKQANLDVVILEVGLGGRLDATNIVD
ADVAVITSIALDHTDWLGPDRESIGREKAGIFRAEKPAIVGEPEMPATIADVAQETGALLRRRGVDWRYEVTATHWAFTD
GDGTLAGLPLPQVPQPNAATALAALRASRLNIDEQAIRDGIAQATLPGRFQIVSESPRVIFDVAHNPHAAEYLTGRLKML
PKRGRVLAVIGMLHDKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGKGNVYDSVAQAWQAAIDAAQPEDTVLV
CGSFHTVAHVMEVIDAGRIGGE

Sequences:

>Translated_422_residues
MKNKSIPQAASPLASWLSYLENLHSKSIDLGLERVSQVAARLDILKPAPFVFTVAGTNGKGTTCRTLESVLIAAGYRVGV
YSSPHLVRYTERVRVQGKELAESAHTASFAEIETARGDISLTYFEYGTLSALWLFKQANLDVVILEVGLGGRLDATNIVD
ADVAVITSIALDHTDWLGPDRESIGREKAGIFRAEKPAIVGEPEMPATIADVAQETGALLRRRGVDWRYEVTATHWAFTD
GDGTLAGLPLPQVPQPNAATALAALRASRLNIDEQAIRDGIAQATLPGRFQIVSESPRVIFDVAHNPHAAEYLTGRLKML
PKRGRVLAVIGMLHDKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGKGNVYDSVAQAWQAAIDAAQPEDTVLV
CGSFHTVAHVMEVIDAGRIGGE
>Mature_422_residues
MKNKSIPQAASPLASWLSYLENLHSKSIDLGLERVSQVAARLDILKPAPFVFTVAGTNGKGTTCRTLESVLIAAGYRVGV
YSSPHLVRYTERVRVQGKELAESAHTASFAEIETARGDISLTYFEYGTLSALWLFKQANLDVVILEVGLGGRLDATNIVD
ADVAVITSIALDHTDWLGPDRESIGREKAGIFRAEKPAIVGEPEMPATIADVAQETGALLRRRGVDWRYEVTATHWAFTD
GDGTLAGLPLPQVPQPNAATALAALRASRLNIDEQAIRDGIAQATLPGRFQIVSESPRVIFDVAHNPHAAEYLTGRLKML
PKRGRVLAVIGMLHDKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGKGNVYDSVAQAWQAAIDAAQPEDTVLV
CGSFHTVAHVMEVIDAGRIGGE

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=205, Percent_Identity=36.5853658536585, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI66932990, Length=205, Percent_Identity=36.5853658536585, Blast_Score=117, Evalue=3e-26,
Organism=Escherichia coli, GI1788654, Length=422, Percent_Identity=86.9668246445498, Blast_Score=728, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17553148, Length=440, Percent_Identity=27.5, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71984923, Length=441, Percent_Identity=27.6643990929705, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17553150, Length=441, Percent_Identity=27.437641723356, Blast_Score=119, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6323760, Length=352, Percent_Identity=28.6931818181818, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324815, Length=322, Percent_Identity=27.0186335403727, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24641571, Length=295, Percent_Identity=30.1694915254237, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24581568, Length=200, Percent_Identity=36, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 45511; Mature: 45511

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKSIPQAASPLASWLSYLENLHSKSIDLGLERVSQVAARLDILKPAPFVFTVAGTNGK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
GTTCRTLESVLIAAGYRVGVYSSPHLVRYTERVRVQGKELAESAHTASFAEIETARGDIS
CCHHHHHHHHHHHCCCEEECCCCCCCEEHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEE
LTYFEYGTLSALWLFKQANLDVVILEVGLGGRLDATNIVDADVAVITSIALDHTDWLGPD
EEEEECCCHHHHEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
RESIGREKAGIFRAEKPAIVGEPEMPATIADVAQETGALLRRRGVDWRYEVTATHWAFTD
HHHHCCHHHCCEECCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEC
GDGTLAGLPLPQVPQPNAATALAALRASRLNIDEQAIRDGIAQATLPGRFQIVSESPRVI
CCCCEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCHHHCCCCCCEEEECCCCCEE
FDVAHNPHAAEYLTGRLKMLPKRGRVLAVIGMLHDKDIAGTLAWLKSVVDDWYCAPLEGP
EEECCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEECCCCCC
RGATAEQLLEHLGKGNVYDSVAQAWQAAIDAAQPEDTVLVCGSFHTVAHVMEVIDAGRIG
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHCCCCC
GE
CC
>Mature Secondary Structure
MKNKSIPQAASPLASWLSYLENLHSKSIDLGLERVSQVAARLDILKPAPFVFTVAGTNGK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
GTTCRTLESVLIAAGYRVGVYSSPHLVRYTERVRVQGKELAESAHTASFAEIETARGDIS
CCHHHHHHHHHHHCCCEEECCCCCCCEEHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEE
LTYFEYGTLSALWLFKQANLDVVILEVGLGGRLDATNIVDADVAVITSIALDHTDWLGPD
EEEEECCCHHHHEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
RESIGREKAGIFRAEKPAIVGEPEMPATIADVAQETGALLRRRGVDWRYEVTATHWAFTD
HHHHCCHHHCCEECCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEC
GDGTLAGLPLPQVPQPNAATALAALRASRLNIDEQAIRDGIAQATLPGRFQIVSESPRVI
CCCCEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCHHHCCCCCCEEEECCCCCEE
FDVAHNPHAAEYLTGRLKMLPKRGRVLAVIGMLHDKDIAGTLAWLKSVVDDWYCAPLEGP
EEECCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEECCCCCC
RGATAEQLLEHLGKGNVYDSVAQAWQAAIDAAQPEDTVLVCGSFHTVAHVMEVIDAGRIG
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHCCCCC
GE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]