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The map label for this gene is leuO [H]

Identifier: 194734337

GI number: 194734337

Start: 33368

End: 34372

Strand: Direct

Name: leuO [H]

Synonym: SeSA_A0033

Alternate gene names: 194734337

Gene position: 33368-34372 (Clockwise)

Preceding gene: 194734689

Following gene: 194735377

Centisome position: 0.71

GC content: 38.91

Gene sequence:

>1005_bases
ATGCGGCCAATAAAAAATGCTAAAAAAATTGACTACAATCTGATCAAAGTATTCGATACGGTTATTACTGAAGGAAATGC
AACCAGGGCGGCGAGGAAACTGGATGTCACGCCTGCGGCGATCTCTCAGGCTCTTCTTCGCTTACAAAATCTTTATGGCG
AAGAGTTATTTATCAGAACCCGCAAAGGATTAGTCCCGTCCAGCAAAGGTAAATCGCTCCACCAGGTATTTCGCCAGGCA
ATTGAATCTATAGAAAACACACTTTGCGATAAAACAGATGCTCAGGAGAGTAATGAACTCATTGTTCTGGGGAGTGATAT
CACTGAAAATTACTATTTTCCGGCGTTGCTGGATATGATGTTGGTGAATCGATATATTATTAAACACTATGCGATTAAAA
AAACAGGGGAATACTCGCCAGCCTCCATGCTGACGCATGGTTATGCGGATGTCATCATGGGAATTCTGGATATTAAGAGT
GAGATGATCGAAAGTTATCTTATTGATAATTTATCTGATTTTGTTTGTGTTTGTGGTGAAAAAAGTCCATTGGTTGGGCT
TGAAAAAATTTCTTTATATAATTTTTATGCTGCCAGACATGCTGTCTATCATTCAGATATGTTCTCTTCTTTCACCGCTG
ATAGCATTGATTTATTCAAGAGCAGTACGCCTTATGCGGGGCGCAGGGAAATAGGTTATTATAGTGATTCACTATTTGGA
GTTATCGGTGTTGTTGAGAAAAGCGATATGGTTGCGATTTTGCCAGGAAAGATTGCTACTTATTTTAGAGATGTACGGCG
TTATAATATAAAAATACTACGTATGCCTGATGAAATAATTTTTCGTACGTTACCCGTTTATGCTTATCTGGCTACAAACA
GCATCCATTATAAAAATGCCAAAAAACTGATATCAACATTTCAGTCGACCTTTCTTTTTAGCCAGGAAAAGCAGCCTGAC
GCTTTGGTTGAAGGAAGCACATCCTTATGCGATTTGTCGGTTTAA

Upstream 100 bases:

>100_bases
ACAGGTAGAGGAAATGATTTACTTATTGAAATAAGTGTGGTGCAAGGTTAATCTGCGCTTTAAATGATGGTGCCAGATTA
TACCAGCCAGGAAAACCTAT

Downstream 100 bases:

>100_bases
TATCAAATTATCGTATTGAATAAATAACCATTATGACCATGGTCAGTAAAGCGAAAAGTAATGCCATATATTTATACCCT
TCTTTTTTTAAAGAAGAGGG

Product: putative transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MRPIKNAKKIDYNLIKVFDTVITEGNATRAARKLDVTPAAISQALLRLQNLYGEELFIRTRKGLVPSSKGKSLHQVFRQA
IESIENTLCDKTDAQESNELIVLGSDITENYYFPALLDMMLVNRYIIKHYAIKKTGEYSPASMLTHGYADVIMGILDIKS
EMIESYLIDNLSDFVCVCGEKSPLVGLEKISLYNFYAARHAVYHSDMFSSFTADSIDLFKSSTPYAGRREIGYYSDSLFG
VIGVVEKSDMVAILPGKIATYFRDVRRYNIKILRMPDEIIFRTLPVYAYLATNSIHYKNAKKLISTFQSTFLFSQEKQPD
ALVEGSTSLCDLSV

Sequences:

>Translated_334_residues
MRPIKNAKKIDYNLIKVFDTVITEGNATRAARKLDVTPAAISQALLRLQNLYGEELFIRTRKGLVPSSKGKSLHQVFRQA
IESIENTLCDKTDAQESNELIVLGSDITENYYFPALLDMMLVNRYIIKHYAIKKTGEYSPASMLTHGYADVIMGILDIKS
EMIESYLIDNLSDFVCVCGEKSPLVGLEKISLYNFYAARHAVYHSDMFSSFTADSIDLFKSSTPYAGRREIGYYSDSLFG
VIGVVEKSDMVAILPGKIATYFRDVRRYNIKILRMPDEIIFRTLPVYAYLATNSIHYKNAKKLISTFQSTFLFSQEKQPD
ALVEGSTSLCDLSV
>Mature_334_residues
MRPIKNAKKIDYNLIKVFDTVITEGNATRAARKLDVTPAAISQALLRLQNLYGEELFIRTRKGLVPSSKGKSLHQVFRQA
IESIENTLCDKTDAQESNELIVLGSDITENYYFPALLDMMLVNRYIIKHYAIKKTGEYSPASMLTHGYADVIMGILDIKS
EMIESYLIDNLSDFVCVCGEKSPLVGLEKISLYNFYAARHAVYHSDMFSSFTADSIDLFKSSTPYAGRREIGYYSDSLFG
VIGVVEKSDMVAILPGKIATYFRDVRRYNIKILRMPDEIIFRTLPVYAYLATNSIHYKNAKKLISTFQSTFLFSQEKQPD
ALVEGSTSLCDLSV

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=280, Percent_Identity=24.2857142857143, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI87081766, Length=264, Percent_Identity=24.6212121212121, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786820, Length=271, Percent_Identity=21.7712177121771, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 37718; Mature: 37718

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPIKNAKKIDYNLIKVFDTVITEGNATRAARKLDVTPAAISQALLRLQNLYGEELFIRT
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEH
RKGLVPSSKGKSLHQVFRQAIESIENTLCDKTDAQESNELIVLGSDITENYYFPALLDMM
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHH
LVNRYIIKHYAIKKTGEYSPASMLTHGYADVIMGILDIKSEMIESYLIDNLSDFVCVCGE
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCC
KSPLVGLEKISLYNFYAARHAVYHSDMFSSFTADSIDLFKSSTPYAGRREIGYYSDSLFG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCHHHHHH
VIGVVEKSDMVAILPGKIATYFRDVRRYNIKILRMPDEIIFRTLPVYAYLATNSIHYKNA
HHHEECCCCEEEEECHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHEEEECCCEEHHHH
KKLISTFQSTFLFSQEKQPDALVEGSTSLCDLSV
HHHHHHHHHHHHHCCCCCCCHHHCCCCCEEECCC
>Mature Secondary Structure
MRPIKNAKKIDYNLIKVFDTVITEGNATRAARKLDVTPAAISQALLRLQNLYGEELFIRT
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEH
RKGLVPSSKGKSLHQVFRQAIESIENTLCDKTDAQESNELIVLGSDITENYYFPALLDMM
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHH
LVNRYIIKHYAIKKTGEYSPASMLTHGYADVIMGILDIKSEMIESYLIDNLSDFVCVCGE
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCC
KSPLVGLEKISLYNFYAARHAVYHSDMFSSFTADSIDLFKSSTPYAGRREIGYYSDSLFG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCHHHHHH
VIGVVEKSDMVAILPGKIATYFRDVRRYNIKILRMPDEIIFRTLPVYAYLATNSIHYKNA
HHHEECCCCEEEEECHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHEEEECCCEEHHHH
KKLISTFQSTFLFSQEKQPDALVEGSTSLCDLSV
HHHHHHHHHHHHHCCCCCCCHHHCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3519576 [H]