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The map label for this gene is ybbB [H]

Identifier: 194734279

GI number: 194734279

Start: 581924

End: 583009

Strand: Reverse

Name: ybbB [H]

Synonym: SeSA_A0577

Alternate gene names: 194734279

Gene position: 583009-581924 (Counterclockwise)

Preceding gene: 194736649

Following gene: 194738202

Centisome position: 12.38

GC content: 55.99

Gene sequence:

>1086_bases
ATGAACGATGGAACGGACTATCGCGCGCTCCTCCTTGCCGATACGCCTTTAATTGATGTACGCGCGCCGATTGAGTTTGA
ACAGGGCGCCATGCCGGGCGCCATCAACCTGCCATTGATGATGGACGACGAGCGAGCCGCCGTCGGCACCTGCTACAAAC
GTCAGGGCGCGGATGCGGCGTTAGCGTTGGGGCATCGGCTGGTTTGCGGCGACATTCGCCAGCAGCGGCTTGAGGCCTGG
AAAGCCGCGTATCAACGTTTCCCCAATGGGTATCTGTGCTGCGCGCGCGGCGGTCAGCGGTCACATATCGTACAACGTTG
GCTGCAAGAAACAGGGATTGACTGCCCGCTTATTGAAGGCGGCTATAAAGCGCTGCGCCAGACAGCAATTCAGGCGACCT
GGCAACTGGCGCAAAAACCGATACTTTTAATTGGCGGCTGTACGGGCAGCGGCAAAACGCAACTGGTGCGACAACAGCCC
AACGGCGTGGATCTGGAAGGTCTCGCGCGTCATCGCGGCTCCTCTTTTGGCCGCACGCTGAAACCCCAACTCAGCCAGGC
CAGTTTTGAAAACAAACTTGCCGTTGAATTGCTGAAAATTAACGCGCGTCAGACGCTAAAAAGGTGGGTACTGGAAGATG
AAGGGCGAACGATCGGCGCCAACCATCTGCCTGAGTGTTTACGCGAAAGAATGGTGCAGGCCCCTATTGCGGTAGTGGAA
GATCCGTTTTCGCTTCGTCTGGAACGACTGCGTGAAGAGTATTTCATCCGTATGCATCACGACTTCACCCACGCCTACGG
CGATGAAGCCGGTTGGCAGGCGTATAGTGAATATCTTCATCATGGACTGTTCGCTATTCGCCGCCGTCTGGGGTTGCAAC
GCTTTGCCGAACTGACGGACACGCTGGATAGGGCGCTGGCGGAGCAATTATCCAGCGGCAGCACCGACGGCCATATGGCA
TGGCTGGTTCCGCTACTTAACGAATATTACGACCCAATGTATCGCTATCAACTGGAGAAAAAAGCCGCGAATATCGTTTT
TCGCGGCACATGGCAAGACGTTGCCAACTGGCTAAAAGCGCAATAG

Upstream 100 bases:

>100_bases
AATAACACATAAAATGCCGGGAGAATGATCTTCCCCTTTCCCGGCTCCTCCCTCAGCGCGTAAAATCTCGCTATTGCTGT
TTTGTTTTTCGGGATTACAC

Downstream 100 bases:

>100_bases
CTTATTTTTATTGCCGGCTGACGCTACATTGAGCCGGCTTAGTGTCTGTCGCGCTTGTCGGCACGTTTTGCCAGCCAGTC
GCCCAGCATCTGAACGACCT

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MNDGTDYRALLLADTPLIDVRAPIEFEQGAMPGAINLPLMMDDERAAVGTCYKRQGADAALALGHRLVCGDIRQQRLEAW
KAAYQRFPNGYLCCARGGQRSHIVQRWLQETGIDCPLIEGGYKALRQTAIQATWQLAQKPILLIGGCTGSGKTQLVRQQP
NGVDLEGLARHRGSSFGRTLKPQLSQASFENKLAVELLKINARQTLKRWVLEDEGRTIGANHLPECLRERMVQAPIAVVE
DPFSLRLERLREEYFIRMHHDFTHAYGDEAGWQAYSEYLHHGLFAIRRRLGLQRFAELTDTLDRALAEQLSSGSTDGHMA
WLVPLLNEYYDPMYRYQLEKKAANIVFRGTWQDVANWLKAQ

Sequences:

>Translated_361_residues
MNDGTDYRALLLADTPLIDVRAPIEFEQGAMPGAINLPLMMDDERAAVGTCYKRQGADAALALGHRLVCGDIRQQRLEAW
KAAYQRFPNGYLCCARGGQRSHIVQRWLQETGIDCPLIEGGYKALRQTAIQATWQLAQKPILLIGGCTGSGKTQLVRQQP
NGVDLEGLARHRGSSFGRTLKPQLSQASFENKLAVELLKINARQTLKRWVLEDEGRTIGANHLPECLRERMVQAPIAVVE
DPFSLRLERLREEYFIRMHHDFTHAYGDEAGWQAYSEYLHHGLFAIRRRLGLQRFAELTDTLDRALAEQLSSGSTDGHMA
WLVPLLNEYYDPMYRYQLEKKAANIVFRGTWQDVANWLKAQ
>Mature_361_residues
MNDGTDYRALLLADTPLIDVRAPIEFEQGAMPGAINLPLMMDDERAAVGTCYKRQGADAALALGHRLVCGDIRQQRLEAW
KAAYQRFPNGYLCCARGGQRSHIVQRWLQETGIDCPLIEGGYKALRQTAIQATWQLAQKPILLIGGCTGSGKTQLVRQQP
NGVDLEGLARHRGSSFGRTLKPQLSQASFENKLAVELLKINARQTLKRWVLEDEGRTIGANHLPECLRERMVQAPIAVVE
DPFSLRLERLREEYFIRMHHDFTHAYGDEAGWQAYSEYLHHGLFAIRRRLGLQRFAELTDTLDRALAEQLSSGSTDGHMA
WLVPLLNEYYDPMYRYQLEKKAANIVFRGTWQDVANWLKAQ

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=356, Percent_Identity=78.6516853932584, Blast_Score=591, Evalue=1e-170,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: NA

Molecular weight: Translated: 40919; Mature: 40919

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDGTDYRALLLADTPLIDVRAPIEFEQGAMPGAINLPLMMDDERAAVGTCYKRQGADAA
CCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCCCHH
LALGHRLVCGDIRQQRLEAWKAAYQRFPNGYLCCARGGQRSHIVQRWLQETGIDCPLIEG
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEHH
GYKALRQTAIQATWQLAQKPILLIGGCTGSGKTQLVRQQPNGVDLEGLARHRGSSFGRTL
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHCCCCCCCCHHHHHHHCCCCCCCCC
KPQLSQASFENKLAVELLKINARQTLKRWVLEDEGRTIGANHLPECLRERMVQAPIAVVE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHCCHHHHC
DPFSLRLERLREEYFIRMHHDFTHAYGDEAGWQAYSEYLHHGLFAIRRRLGLQRFAELTD
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLDRALAEQLSSGSTDGHMAWLVPLLNEYYDPMYRYQLEKKAANIVFRGTWQDVANWLKA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCEEEECCHHHHHHHHCC
Q
C
>Mature Secondary Structure
MNDGTDYRALLLADTPLIDVRAPIEFEQGAMPGAINLPLMMDDERAAVGTCYKRQGADAA
CCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCCCHH
LALGHRLVCGDIRQQRLEAWKAAYQRFPNGYLCCARGGQRSHIVQRWLQETGIDCPLIEG
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEHH
GYKALRQTAIQATWQLAQKPILLIGGCTGSGKTQLVRQQPNGVDLEGLARHRGSSFGRTL
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHCCCCCCCCHHHHHHHCCCCCCCCC
KPQLSQASFENKLAVELLKINARQTLKRWVLEDEGRTIGANHLPECLRERMVQAPIAVVE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHCCHHHHC
DPFSLRLERLREEYFIRMHHDFTHAYGDEAGWQAYSEYLHHGLFAIRRRLGLQRFAELTD
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLDRALAEQLSSGSTDGHMAWLVPLLNEYYDPMYRYQLEKKAANIVFRGTWQDVANWLKA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCEEEECCHHHHHHHHCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA