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The map label for this gene is cof [H]

Identifier: 194734264

GI number: 194734264

Start: 522357

End: 523175

Strand: Direct

Name: cof [H]

Synonym: SeSA_A0516

Alternate gene names: 194734264

Gene position: 522357-523175 (Clockwise)

Preceding gene: 194734324

Following gene: 194734165

Centisome position: 11.09

GC content: 54.21

Gene sequence:

>819_bases
ATGGCCCGTCTGGCAGCATTTGATATGGATGGCACGTTATTAATGCCCGATCACCATTTGGGTAGAGAAACGATCGCCAC
GCTGGCGCGTCTGCGCGAACGCGATATCACTCTGACATTCGCTACGGGTCGTCATGTGCTGGAGATGCGGCATATTCTGG
GGACGCTTTCACTGGACGCTTATCTTATTACCGGTAACGGGACGCGAATTCACTCGCTGGAAGGCGACGTGCTGCATCGT
CAGGATCTCGATCCTCAGGTTGCCGATACGGTGATGCATCATGCCTGGGATACCCGAGCCAGTATGCATGTGTTTAATGA
CAACGGCTGGTTTACAGGCCAGGAGATCCCCGCGCTACTCCAGGCGCACGTCTACAGCGGCTTTCGTTATCAGGTTATCA
ATATAAAAAGCATTCCGGCCCATCAGGTGACGAAGATCTGTTTTTGCGGCGATCATGACGATCTGATCCGTTTGCGCATT
CAGCTCAATGAGGCGATGGAAGAACGCGCTCACCTCTGTTTCTCTGCCGTCGACTGCCTGGAAGTGCTACCGCTGGGGTG
CAATAAAGGATCGGCGCTGGCGGTATTGAGCAATCATTTAGGGCTTTCACTGGCAGATTGCATGGCGTTTGGCGACGCGA
TGAACGATCGGGAAATGCTGGGCAGCGTCGGGCGGGGGCTCATTATGGGCAATGCCATGCCGCAGCTTATCGCCGCGCTG
CCACACCTTGCGGTTATCGGACATTGTGGTAATCAAGCGGTATCACACTTTTTGACGCATTGGCTGGACAATCCGCATCT
ACCTTATTCCCCCGAATGA

Upstream 100 bases:

>100_bases
TAAAACCCGGAACACTTCCTTTTATCTGTTCACTATTACTTCCGTATATCTCAGGTAATACTGAACAACAAGTTAACCGC
GTCACAGTGTGGAGAAAGAG

Downstream 100 bases:

>100_bases
GAGATCTCTTCCAGCAAGCCAGACATTCAGTCTGGCTTTTTTTTGTTTCTAGTCGCCGGTAAGTAATTTTGCGATGGCCG
CTGACCACGGCGTCAAATCG

Product: protein cof

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MARLAAFDMDGTLLMPDHHLGRETIATLARLRERDITLTFATGRHVLEMRHILGTLSLDAYLITGNGTRIHSLEGDVLHR
QDLDPQVADTVMHHAWDTRASMHVFNDNGWFTGQEIPALLQAHVYSGFRYQVINIKSIPAHQVTKICFCGDHDDLIRLRI
QLNEAMEERAHLCFSAVDCLEVLPLGCNKGSALAVLSNHLGLSLADCMAFGDAMNDREMLGSVGRGLIMGNAMPQLIAAL
PHLAVIGHCGNQAVSHFLTHWLDNPHLPYSPE

Sequences:

>Translated_272_residues
MARLAAFDMDGTLLMPDHHLGRETIATLARLRERDITLTFATGRHVLEMRHILGTLSLDAYLITGNGTRIHSLEGDVLHR
QDLDPQVADTVMHHAWDTRASMHVFNDNGWFTGQEIPALLQAHVYSGFRYQVINIKSIPAHQVTKICFCGDHDDLIRLRI
QLNEAMEERAHLCFSAVDCLEVLPLGCNKGSALAVLSNHLGLSLADCMAFGDAMNDREMLGSVGRGLIMGNAMPQLIAAL
PHLAVIGHCGNQAVSHFLTHWLDNPHLPYSPE
>Mature_271_residues
ARLAAFDMDGTLLMPDHHLGRETIATLARLRERDITLTFATGRHVLEMRHILGTLSLDAYLITGNGTRIHSLEGDVLHRQ
DLDPQVADTVMHHAWDTRASMHVFNDNGWFTGQEIPALLQAHVYSGFRYQVINIKSIPAHQVTKICFCGDHDDLIRLRIQ
LNEAMEERAHLCFSAVDCLEVLPLGCNKGSALAVLSNHLGLSLADCMAFGDAMNDREMLGSVGRGLIMGNAMPQLIAALP
HLAVIGHCGNQAVSHFLTHWLDNPHLPYSPE

Specific function: Catalyzes the hydrolysis of 4-amino-2-methyl-5- hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2- methyl-5-hydroxymethylpyrimidine phosphate (HMP-P) [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081741, Length=272, Percent_Identity=80.5147058823529, Blast_Score=463, Evalue=1e-132,
Organism=Escherichia coli, GI48994981, Length=260, Percent_Identity=37.3076923076923, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1786982, Length=278, Percent_Identity=28.0575539568345, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF08282 Hydrolase_3 [H]

EC number: NA

Molecular weight: Translated: 30105; Mature: 29974

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS01228 COF_1 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLAAFDMDGTLLMPDHHLGRETIATLARLRERDITLTFATGRHVLEMRHILGTLSLDA
CCCEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHCEEE
YLITGNGTRIHSLEGDVLHRQDLDPQVADTVMHHAWDTRASMHVFNDNGWFTGQEIPALL
EEEECCCCEEEECCCCCHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEECCHHHHHHH
QAHVYSGFRYQVINIKSIPAHQVTKICFCGDHDDLIRLRIQLNEAMEERAHLCFSAVDCL
HHHHHCCCEEEEEEECCCCHHHHEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHH
EVLPLGCNKGSALAVLSNHLGLSLADCMAFGDAMNDREMLGSVGRGLIMGNAMPQLIAAL
HHCCCCCCCCCEEEEEHHCCCCHHHHHHHHHCCCCHHHHHHHHCCCEEECCHHHHHHHHH
PHLAVIGHCGNQAVSHFLTHWLDNPHLPYSPE
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ARLAAFDMDGTLLMPDHHLGRETIATLARLRERDITLTFATGRHVLEMRHILGTLSLDA
CCEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHCEEE
YLITGNGTRIHSLEGDVLHRQDLDPQVADTVMHHAWDTRASMHVFNDNGWFTGQEIPALL
EEEECCCCEEEECCCCCHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEECCHHHHHHH
QAHVYSGFRYQVINIKSIPAHQVTKICFCGDHDDLIRLRIQLNEAMEERAHLCFSAVDCL
HHHHHCCCEEEEEEECCCCHHHHEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHH
EVLPLGCNKGSALAVLSNHLGLSLADCMAFGDAMNDREMLGSVGRGLIMGNAMPQLIAAL
HHCCCCCCCCCEEEEEHHCCCCHHHHHHHHHCCCCHHHHHHHHCCCEEECCHHHHHHHHH
PHLAVIGHCGNQAVSHFLTHWLDNPHLPYSPE
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA