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The map label for this gene is rfbJ [H]

Identifier: 194734104

GI number: 194734104

Start: 2191541

End: 2192440

Strand: Reverse

Name: rfbJ [H]

Synonym: SeSA_A2322

Alternate gene names: 194734104

Gene position: 2192440-2191541 (Counterclockwise)

Preceding gene: 194734744

Following gene: 194738297

Centisome position: 46.56

GC content: 31.78

Gene sequence:

>900_bases
ATGACCTTTTTGAAAGAATATGTAATTGTCAGTGGGGCTTCCGGCTTTATTGGTAAGCATTTACTCGAAGCTCTAAAAAA
ATCGGGGATTTCAGTTGTCGCAATCACTCGAGATGTAATAAAAAATAATAGTAATGCATTAGCTAATGTTAGATGGTGCT
GTTGGGATAATATCGAATTATTAGTGGAGGAGTTATCAATTGATTCTGCATTAATTGGTATCATTCATTTGGCAACAGAA
TATGGGCATAAAACATCATCTCTCATAAATATTGAAGATGCAAATGTTATAAAACCATTAAAGCTTCTTGATTTGGCAAT
AAAATATCGGGCGAATATCTTTTTAAATACAGATAGTTTTTTTGCCAAGAAAGATTTTAATTATCAACATATGCGGCCTT
ATATAATTACTAAAAGACACTTTGATGAAATTGGGCATTATTATGCTAATATGCATGACATTTCATTTGTAAACATGCGA
TTAGAGCATGTATATGGGCCTGGGGATGGTGAAAATAAATTTATTCCATACATTATCGACTGCTTAAATAAAAAACAGAG
TTGTGTGAAATGTACAACAGGCGAACAGATAAGAGACTTTATTTTTGTAGATGATGTGGTAAATGCTTATTTAACTATAT
TAGAAAATAGAAAAAAAGTACCTTCATATACTGAGTATCAAGTTGGAACTGGTGCTGGGGTAAGTTTGAAGGATTTTCTG
GTTTATTTGCAAAATACTATGATGCCAGGTTCATCGAGTATATTTGAATTCGGTGCGATAGAGCAAAGAGATAATGAAAT
AATGTTCTCTGTTGCAAATAATAAAAATTTAAAAGCAATGGGCTGGAAACCAAATTTCGATTATAAAAAAGGAATTGAAG
AACTACTGAAACGGTTATGA

Upstream 100 bases:

>100_bases
TCCAGGCTTGACTACAGAGCATTTAGATTATGTAGTTAGCAAGTTTGAAGAGTTCTTTGGTTTAAATTTCTAATTTAATC
TATTCTATCTGGTGATTGCG

Downstream 100 bases:

>100_bases
GATTTTCATGATCTTTTAATAAATAAATCGTTAACAAATTAGTCGCGTTATGTTGTAAAAACTAAGTCGTTTAATTGCAT
AGTGAAAGTTCAATTGTTAA

Product: CDP-abequose synthase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: O4 antigen [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTFLKEYVIVSGASGFIGKHLLEALKKSGISVVAITRDVIKNNSNALANVRWCCWDNIELLVEELSIDSALIGIIHLATE
YGHKTSSLINIEDANVIKPLKLLDLAIKYRANIFLNTDSFFAKKDFNYQHMRPYIITKRHFDEIGHYYANMHDISFVNMR
LEHVYGPGDGENKFIPYIIDCLNKKQSCVKCTTGEQIRDFIFVDDVVNAYLTILENRKKVPSYTEYQVGTGAGVSLKDFL
VYLQNTMMPGSSSIFEFGAIEQRDNEIMFSVANNKNLKAMGWKPNFDYKKGIEELLKRL

Sequences:

>Translated_299_residues
MTFLKEYVIVSGASGFIGKHLLEALKKSGISVVAITRDVIKNNSNALANVRWCCWDNIELLVEELSIDSALIGIIHLATE
YGHKTSSLINIEDANVIKPLKLLDLAIKYRANIFLNTDSFFAKKDFNYQHMRPYIITKRHFDEIGHYYANMHDISFVNMR
LEHVYGPGDGENKFIPYIIDCLNKKQSCVKCTTGEQIRDFIFVDDVVNAYLTILENRKKVPSYTEYQVGTGAGVSLKDFL
VYLQNTMMPGSSSIFEFGAIEQRDNEIMFSVANNKNLKAMGWKPNFDYKKGIEELLKRL
>Mature_298_residues
TFLKEYVIVSGASGFIGKHLLEALKKSGISVVAITRDVIKNNSNALANVRWCCWDNIELLVEELSIDSALIGIIHLATEY
GHKTSSLINIEDANVIKPLKLLDLAIKYRANIFLNTDSFFAKKDFNYQHMRPYIITKRHFDEIGHYYANMHDISFVNMRL
EHVYGPGDGENKFIPYIIDCLNKKQSCVKCTTGEQIRDFIFVDDVVNAYLTILENRKKVPSYTEYQVGTGAGVSLKDFLV
YLQNTMMPGSSSIFEFGAIEQRDNEIMFSVANNKNLKAMGWKPNFDYKKGIEELLKRL

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI7657641, Length=321, Percent_Identity=24.9221183800623, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 34121; Mature: 33990

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFLKEYVIVSGASGFIGKHLLEALKKSGISVVAITRDVIKNNSNALANVRWCCWDNIEL
CCHHHCCCEEECCCHHHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCEEEEEEEEECCHHH
LVEELSIDSALIGIIHLATEYGHKTSSLINIEDANVIKPLKLLDLAIKYRANIFLNTDSF
HHHHHCCCHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHEEEEEEEECCCC
FAKKDFNYQHMRPYIITKRHFDEIGHYYANMHDISFVNMRLEHVYGPGDGENKFIPYIID
EEECCCCHHHCCCEEEEHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCCCCHHHHHH
CLNKKQSCVKCTTGEQIRDFIFVDDVVNAYLTILENRKKVPSYTEYQVGTGAGVSLKDFL
HHCCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCHHHHH
VYLQNTMMPGSSSIFEFGAIEQRDNEIMFSVANNKNLKAMGWKPNFDYKKGIEELLKRL
HHHHHHCCCCCCCCEECCCCCCCCCEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHC
>Mature Secondary Structure 
TFLKEYVIVSGASGFIGKHLLEALKKSGISVVAITRDVIKNNSNALANVRWCCWDNIEL
CHHHCCCEEECCCHHHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCEEEEEEEEECCHHH
LVEELSIDSALIGIIHLATEYGHKTSSLINIEDANVIKPLKLLDLAIKYRANIFLNTDSF
HHHHHCCCHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHEEEEEEEECCCC
FAKKDFNYQHMRPYIITKRHFDEIGHYYANMHDISFVNMRLEHVYGPGDGENKFIPYIID
EEECCCCHHHCCCEEEEHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCCCCHHHHHH
CLNKKQSCVKCTTGEQIRDFIFVDDVVNAYLTILENRKKVPSYTEYQVGTGAGVSLKDFL
HHCCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCHHHHH
VYLQNTMMPGSSSIFEFGAIEQRDNEIMFSVANNKNLKAMGWKPNFDYKKGIEELLKRL
HHHHHHCCCCCCCCEECCCCCCCCCEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2793832; 1710759; 11677609 [H]