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The map label for this gene is sifA [H]

Identifier: 194734056

GI number: 194734056

Start: 1274489

End: 1275499

Strand: Reverse

Name: sifA [H]

Synonym: SeSA_A1302

Alternate gene names: 194734056

Gene position: 1275499-1274489 (Counterclockwise)

Preceding gene: 194734735

Following gene: 194737971

Centisome position: 27.09

GC content: 40.16

Gene sequence:

>1011_bases
ATGCCGATTACTATAGGGAATGGTTTTTTAAAAAGTGAAATCCTTACCAACTCCCCAAGGAATACGAAAGAAGCATGGTG
GAAAGTTTTATGGGAAAAAATTAAAGACTTCTTTTTTTCTACTGGCAAAGCAAAAGCGGACAGTTGTCTACATGAGATGT
TGTTTGCCGATCGCACCCCCACACGAGAGCGGCTTACAGAGATTTTTTTTGAGTTAAAAGAGTTAGCCTGCGCATCACAA
AGAGATAGATTTCAGGTTTATAATCCTAATGAAAATGATGACACTATTATTCTTCGCATCATGGATCAAAACGAAGAGAA
CGAATTGTTACGTATCACTCAAAATACCGATGCCTTTACCTGTGAAGTCATGGGGAATGCTTATTTTTTAATAAAAGATC
GCCCCGATATTTTAAAACCGCATCCACAAATGACGGCCATGATTAATAGAAGATATAGCGAAATCGTAGATTACCCCCTC
CCTTCGACATTATGTCTCAATCCTGCTGGCGCGCCGACATTATCGGTTCCATTAGACAACATAAAGGGGTATTTATATTC
TGAATGGAGAAAAGGAAATTTAGATGAGTGGAAAACGCAAGAAAAGGTAACCTACCTGGCAGCGAAAATTCAGTCTGGGA
TTGAAAAGACAACGCGCATTTTACAGCATGCGAATATATCCGAAAGTACTCAGCAAAACGCATTTTTAGAAACAATGACG
ATGTGTGGCTTAAAACAGATTGAAATACCACCACCGCATACCCACATACCTATTGAAAAAATGGTAGAAGAGGTTTTACT
GGCGGATAAGACGTTTCAGGCGTTCCTTGTAACGGATCCCAGCGCCAGCCTAAGCATGTTAGCTGAGATAATCGAAACCC
TCTCTGATCAGGTTTTTCACGCCATTTTTAGAATAGACCCCCAGGCTATACAAAAAATGGCGGAAGAACAGTTAACCACG
CTACACGTTCGCTCAGAACAACAAAGCGGCTGTTTATGTTGTTTTTTATAA

Upstream 100 bases:

>100_bases
TTAAAAATTATATTACATCCGATGCGCCCGCAGTAGAGATAGAAAGGGCCGATTTAATCAATTATGTAGTCATTTTTACT
CCAGTATAAGTGAGATTAAT

Downstream 100 bases:

>100_bases
AATCAGACGACGCTTTCTCAAACGTCACGTTCAGGGTTTACTCACGTACTCCATGTTTGCCTACAGAGTAACCTCTTGCC
TGGCGCAATAATGCCGCATA

Product: secreted effector protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MPITIGNGFLKSEILTNSPRNTKEAWWKVLWEKIKDFFFSTGKAKADSCLHEMLFADRTPTRERLTEIFFELKELACASQ
RDRFQVYNPNENDDTIILRIMDQNEENELLRITQNTDAFTCEVMGNAYFLIKDRPDILKPHPQMTAMINRRYSEIVDYPL
PSTLCLNPAGAPTLSVPLDNIKGYLYSEWRKGNLDEWKTQEKVTYLAAKIQSGIEKTTRILQHANISESTQQNAFLETMT
MCGLKQIEIPPPHTHIPIEKMVEEVLLADKTFQAFLVTDPSASLSMLAEIIETLSDQVFHAIFRIDPQAIQKMAEEQLTT
LHVRSEQQSGCLCCFL

Sequences:

>Translated_336_residues
MPITIGNGFLKSEILTNSPRNTKEAWWKVLWEKIKDFFFSTGKAKADSCLHEMLFADRTPTRERLTEIFFELKELACASQ
RDRFQVYNPNENDDTIILRIMDQNEENELLRITQNTDAFTCEVMGNAYFLIKDRPDILKPHPQMTAMINRRYSEIVDYPL
PSTLCLNPAGAPTLSVPLDNIKGYLYSEWRKGNLDEWKTQEKVTYLAAKIQSGIEKTTRILQHANISESTQQNAFLETMT
MCGLKQIEIPPPHTHIPIEKMVEEVLLADKTFQAFLVTDPSASLSMLAEIIETLSDQVFHAIFRIDPQAIQKMAEEQLTT
LHVRSEQQSGCLCCFL
>Mature_335_residues
PITIGNGFLKSEILTNSPRNTKEAWWKVLWEKIKDFFFSTGKAKADSCLHEMLFADRTPTRERLTEIFFELKELACASQR
DRFQVYNPNENDDTIILRIMDQNEENELLRITQNTDAFTCEVMGNAYFLIKDRPDILKPHPQMTAMINRRYSEIVDYPLP
STLCLNPAGAPTLSVPLDNIKGYLYSEWRKGNLDEWKTQEKVTYLAAKIQSGIEKTTRILQHANISESTQQNAFLETMTM
CGLKQIEIPPPHTHIPIEKMVEEVLLADKTFQAFLVTDPSASLSMLAEIIETLSDQVFHAIFRIDPQAIQKMAEEQLTTL
HVRSEQQSGCLCCFL

Specific function: Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is required for endosomal tubulation and formation of Salmonella- induced filaments (Sifs), which are filamentous structures containing l

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Host cytoplasm. Host cell membrane. Note=Secreted via type III secretion system 2 (SPI-2 TTSS), and delivered into the host cytoplasm. It associates with membranes and colocalizes with microtubules along the length of Sifs in infected cells [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Sif family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010637 [H]

Pfam domain/function: PF06767 Sif [H]

EC number: NA

Molecular weight: Translated: 38587; Mature: 38456

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPITIGNGFLKSEILTNSPRNTKEAWWKVLWEKIKDFFFSTGKAKADSCLHEMLFADRTP
CCEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
TRERLTEIFFELKELACASQRDRFQVYNPNENDDTIILRIMDQNEENELLRITQNTDAFT
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEECCCCCCCEEEEECCCCEEE
CEVMGNAYFLIKDRPDILKPHPQMTAMINRRYSEIVDYPLPSTLCLNPAGAPTLSVPLDN
EEEECCEEEEEECCCHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEECCHHH
IKGYLYSEWRKGNLDEWKTQEKVTYLAAKIQSGIEKTTRILQHANISESTQQNAFLETMT
HCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
MCGLKQIEIPPPHTHIPIEKMVEEVLLADKTFQAFLVTDPSASLSMLAEIIETLSDQVFH
HCCCEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHH
AIFRIDPQAIQKMAEEQLTTLHVRSEQQSGCLCCFL
HHHHCCHHHHHHHHHHHHHEEEEECCCCCCCEEEEC
>Mature Secondary Structure 
PITIGNGFLKSEILTNSPRNTKEAWWKVLWEKIKDFFFSTGKAKADSCLHEMLFADRTP
CEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
TRERLTEIFFELKELACASQRDRFQVYNPNENDDTIILRIMDQNEENELLRITQNTDAFT
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEECCCCCCCEEEEECCCCEEE
CEVMGNAYFLIKDRPDILKPHPQMTAMINRRYSEIVDYPLPSTLCLNPAGAPTLSVPLDN
EEEECCEEEEEECCCHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEECCHHH
IKGYLYSEWRKGNLDEWKTQEKVTYLAAKIQSGIEKTTRILQHANISESTQQNAFLETMT
HCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
MCGLKQIEIPPPHTHIPIEKMVEEVLLADKTFQAFLVTDPSASLSMLAEIIETLSDQVFH
HCCCEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHH
AIFRIDPQAIQKMAEEQLTTLHVRSEQQSGCLCCFL
HHHHCCHHHHHHHHHHHHHEEEEECCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8861213; 11677609; 10861017 [H]