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The map label for this gene is mak [H]

Identifier: 194335111

GI number: 194335111

Start: 2508218

End: 2509114

Strand: Reverse

Name: mak [H]

Synonym: Paes_2333

Alternate gene names: 194335111

Gene position: 2509114-2508218 (Counterclockwise)

Preceding gene: 194335112

Following gene: 194335110

Centisome position: 99.85

GC content: 56.63

Gene sequence:

>897_bases
ATGAAGCGGTTATGGGGTATCGATCTCGGGGGAACAAAGATCGAAGGCGTTGTGCTGGATGAAGCCCTTCGTCCGCTGGT
CAGGATCCGTCTTGCGACAGAAGCATGCAACGGCTACCGTCATGTCCTGCAGCGTATCGTCGAGCTTATCGATCGTATGG
CTCTTCAGGCGGGCACATCTCCGCCATCCACAATAGGGATCGGTACGCCGGGTTCGATCGATGTGAGAACGGGGGTGATC
AAAAACTCCAACACGCTCTGCCTGAACGGCAAACCGTTTCGCTCGGATATCGAGGCTTTGCTTGAGCGGCAGGTTTTGGT
TGACAATGACGCCAACTGTTTTGCGCTTGCCGAGTACCGGATGGGGTCAGGAAAGATGCTCAGCGACAGCGCAACGGTTT
TCGGTATCATTCTCGGTACCGGTGTCGGGGGAGGGATTGTCTGCGGCGGAAGGCTGCATCATGGAGCGCAAGGTATTGCC
GGAGAGTGGGGGCACAATGAGCTGATTCCGGGAGGAGAACCCTGCTACTGCGGCAGACGGGGCTGTGTTGAAACGGTGAT
TTCAGGCCCTGCTCTCGAACGCTACTACTCGAACCTCACCGGACAAGAGAAGGCTCTTGCAGCAATAGCCTCTGGCGATG
ACGATGCCTCGAGGCGCACCATAGCGCGTCTTCAGGAGTATTTCGGCAAGGCGCTTGCAGGTGTTATCAACGTGCTTGAT
CCCGATATGGTTATTATCGGCGGAGGGGTCGGTAACATCGACGCGCTCTATAGCCAAAGCGTCCGATACAGGATAGCGTC
GCACGTGTTTAACGGATCATTCGATACGCCTGTCGTCCGCCCCCGGCTTGGCGACAGTGCCGGAGTTTTCGGGGCAGCAT
TGCTGACAGCGTACTAA

Upstream 100 bases:

>100_bases
TAGCGTGTTCAAGTGCCATCTATCTCTGTTTTGCATGGGTGATCAAAAACGTCATACAGGTCAACGGGATGAAAAACATG
ACCTGAGAAGGAGGATGCAG

Downstream 100 bases:

>100_bases
CAGGAAAAAAGAAGAATAATGACATTACGTGACATCTACCGTAAGGGAGAGCTGGTCGAACTTACGATTGCAGATATAGC
AGAAAAAGATCAGTGTTTCG

Product: ROK family protein

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MKRLWGIDLGGTKIEGVVLDEALRPLVRIRLATEACNGYRHVLQRIVELIDRMALQAGTSPPSTIGIGTPGSIDVRTGVI
KNSNTLCLNGKPFRSDIEALLERQVLVDNDANCFALAEYRMGSGKMLSDSATVFGIILGTGVGGGIVCGGRLHHGAQGIA
GEWGHNELIPGGEPCYCGRRGCVETVISGPALERYYSNLTGQEKALAAIASGDDDASRRTIARLQEYFGKALAGVINVLD
PDMVIIGGGVGNIDALYSQSVRYRIASHVFNGSFDTPVVRPRLGDSAGVFGAALLTAY

Sequences:

>Translated_298_residues
MKRLWGIDLGGTKIEGVVLDEALRPLVRIRLATEACNGYRHVLQRIVELIDRMALQAGTSPPSTIGIGTPGSIDVRTGVI
KNSNTLCLNGKPFRSDIEALLERQVLVDNDANCFALAEYRMGSGKMLSDSATVFGIILGTGVGGGIVCGGRLHHGAQGIA
GEWGHNELIPGGEPCYCGRRGCVETVISGPALERYYSNLTGQEKALAAIASGDDDASRRTIARLQEYFGKALAGVINVLD
PDMVIIGGGVGNIDALYSQSVRYRIASHVFNGSFDTPVVRPRLGDSAGVFGAALLTAY
>Mature_298_residues
MKRLWGIDLGGTKIEGVVLDEALRPLVRIRLATEACNGYRHVLQRIVELIDRMALQAGTSPPSTIGIGTPGSIDVRTGVI
KNSNTLCLNGKPFRSDIEALLERQVLVDNDANCFALAEYRMGSGKMLSDSATVFGIILGTGVGGGIVCGGRLHHGAQGIA
GEWGHNELIPGGEPCYCGRRGCVETVISGPALERYYSNLTGQEKALAAIASGDDDASRRTIARLQEYFGKALAGVINVLD
PDMVIIGGGVGNIDALYSQSVRYRIASHVFNGSFDTPVVRPRLGDSAGVFGAALLTAY

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI298566325, Length=271, Percent_Identity=28.0442804428044, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI298566315, Length=271, Percent_Identity=28.0442804428044, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4885285, Length=271, Percent_Identity=28.0442804428044, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI190014632, Length=271, Percent_Identity=28.0442804428044, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI87081733, Length=302, Percent_Identity=43.3774834437086, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1787363, Length=304, Percent_Identity=32.5657894736842, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1786891, Length=268, Percent_Identity=26.865671641791, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787878, Length=250, Percent_Identity=26, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790522, Length=129, Percent_Identity=36.4341085271318, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 31532; Mature: 31532

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLWGIDLGGTKIEGVVLDEALRPLVRIRLATEACNGYRHVLQRIVELIDRMALQAGTS
CCCEEECCCCCCEEEHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PPSTIGIGTPGSIDVRTGVIKNSNTLCLNGKPFRSDIEALLERQVLVDNDANCFALAEYR
CCCEEECCCCCCEEEEEEEEECCCEEEECCCCCHHHHHHHHHHHHEECCCCCEEEEEEEE
MGSGKMLSDSATVFGIILGTGVGGGIVCGGRLHHGAQGIAGEWGHNELIPGGEPCYCGRR
CCCCCEECCCCCEEEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
GCVETVISGPALERYYSNLTGQEKALAAIASGDDDASRRTIARLQEYFGKALAGVINVLD
HHHHHHHCCHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PDMVIIGGGVGNIDALYSQSVRYRIASHVFNGSFDTPVVRPRLGDSAGVFGAALLTAY
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKRLWGIDLGGTKIEGVVLDEALRPLVRIRLATEACNGYRHVLQRIVELIDRMALQAGTS
CCCEEECCCCCCEEEHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PPSTIGIGTPGSIDVRTGVIKNSNTLCLNGKPFRSDIEALLERQVLVDNDANCFALAEYR
CCCEEECCCCCCEEEEEEEEECCCEEEECCCCCHHHHHHHHHHHHEECCCCCEEEEEEEE
MGSGKMLSDSATVFGIILGTGVGGGIVCGGRLHHGAQGIAGEWGHNELIPGGEPCYCGRR
CCCCCEECCCCCEEEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
GCVETVISGPALERYYSNLTGQEKALAAIASGDDDASRRTIARLQEYFGKALAGVINVLD
HHHHHHHCCHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PDMVIIGGGVGNIDALYSQSVRYRIASHVFNGSFDTPVVRPRLGDSAGVFGAALLTAY
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]