Click here to switch to the map view.

The map label for this gene is aprN [H]

Identifier: 194335068

GI number: 194335068

Start: 2459098

End: 2460297

Strand: Reverse

Name: aprN [H]

Synonym: Paes_2280

Alternate gene names: 194335068

Gene position: 2460297-2459098 (Counterclockwise)

Preceding gene: 194335069

Following gene: 194335060

Centisome position: 97.91

GC content: 53.67

Gene sequence:

>1200_bases
ATGACAGTTGATGCAATCACCATCGTTGTGCGCGACTCTGTCACCGGGGATCCCGTTTCTGGAGCGATGGTCGTGCTCAA
TGGAGATGATCATTCGCTGACAAACAGCGATGGCGTTGCTCGATTCAGACAGCCTTGTTCACGTTCAGATGGTAAATTTC
ACGAATTTCTGATTTTTCGAGGAATGGAATTCAAGCCGTTTCTCCTGTCTGTTTCCCATACCCAACTTGTTGGCATCAGG
ACCTTCCCTGTCCTCCTCGAAGGCCAGCTTCCGCCTCCGGCTATGGGTATCGGATGGGGCTTGCAGGCGATCGGGGCAGA
TGTGTCTCCCCTGCCATCATTTGATGGAATCAAAGTAGGGATTCTTGATACAGGCATAGATTCCTCGCATCCCGGTCTCA
ATGTTATTGATGGATTCAATCAGGGAAATGATGCGTATCCGTTTTCCCGGCCTATTTCTCCGCACGGGACATTCACCGCC
GGATTGATTGGAGGCAAAAGAAGAGGAGATCAGGGTATTGTCGGTGTTGCACCGGGGGTTGATCTTCTCGATATTCGCGT
TTTCGATCCTGCAGGTATAGGTGCGACGAGAGACGTGATCTGCCAGGGCGTTCGTTGGGCGATTTCAAGGGGTGCACAGA
TACTCTATGTTGGATTGGGATCGGAAACGTACAGCGAAATGGAAGAACGGGTTTGGCGCGAAGCCTACGACGCGGGGATC
GTTGTTGTTGCTCCGGCTGGAAACACGATGAGTGACCACAAAGAGGTGCTCTATCCAGCCAGGTACGTTTCGACGATTTG
CGTGGCCGCCTGCGGCAGAAATACGGATGTTCCCCCGGATGAACAGCCCAGACTCTCCAAAGATGGCGCGTGGTTTCTTC
CTTCATTCAGCCGAAGAGGCCCTGAAGTCGATGTCGTCGCGCCGGGAGTTGCCGTCTGCTCCATTGTTCCCGGTCAACCC
GGCGGGAAGACACTCTACGATCACTGCTCCGGGACAAGCCAAGCCGCTGCTTTTGTTGCCGGTGTCGCAGCCAGGATACT
TGCAACGCATCCGGAGATAAAGAACGTGTGTCCGAAAGAAAAGACGGAGCTGGTGCGCGCTTTGCTTCGCACCAATGCAC
TACCGCTTGGTATTACTGGATCTGGCGCCGGATTGTGCCGTCTTGGCGGTAACACTACAAGCCCACTCATCATACAGTAG

Upstream 100 bases:

>100_bases
GGCGATCAGAATCGGGAGGGCGAATCGCAAAATTTGTCCATACTCTTCTCATCAGAGAGGACGGCCCTCTTGTCTTGACG
CAGCCATCAAAAGAGCGATA

Downstream 100 bases:

>100_bases
GGCACCGCTATTCATTTGTTGTGGGACCCGAATACTTCGTTAGTCGGTCCTCGAAATCCTCATCCCGACTTGTCGGGATT
CCGGTTTCTGTGCTCCGTCA

Product: peptidase S8/S53 subtilisin kexin sedolisin

Products: NA

Alternate protein names: Nattokinase [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTVDAITIVVRDSVTGDPVSGAMVVLNGDDHSLTNSDGVARFRQPCSRSDGKFHEFLIFRGMEFKPFLLSVSHTQLVGIR
TFPVLLEGQLPPPAMGIGWGLQAIGADVSPLPSFDGIKVGILDTGIDSSHPGLNVIDGFNQGNDAYPFSRPISPHGTFTA
GLIGGKRRGDQGIVGVAPGVDLLDIRVFDPAGIGATRDVICQGVRWAISRGAQILYVGLGSETYSEMEERVWREAYDAGI
VVVAPAGNTMSDHKEVLYPARYVSTICVAACGRNTDVPPDEQPRLSKDGAWFLPSFSRRGPEVDVVAPGVAVCSIVPGQP
GGKTLYDHCSGTSQAAAFVAGVAARILATHPEIKNVCPKEKTELVRALLRTNALPLGITGSGAGLCRLGGNTTSPLIIQ

Sequences:

>Translated_399_residues
MTVDAITIVVRDSVTGDPVSGAMVVLNGDDHSLTNSDGVARFRQPCSRSDGKFHEFLIFRGMEFKPFLLSVSHTQLVGIR
TFPVLLEGQLPPPAMGIGWGLQAIGADVSPLPSFDGIKVGILDTGIDSSHPGLNVIDGFNQGNDAYPFSRPISPHGTFTA
GLIGGKRRGDQGIVGVAPGVDLLDIRVFDPAGIGATRDVICQGVRWAISRGAQILYVGLGSETYSEMEERVWREAYDAGI
VVVAPAGNTMSDHKEVLYPARYVSTICVAACGRNTDVPPDEQPRLSKDGAWFLPSFSRRGPEVDVVAPGVAVCSIVPGQP
GGKTLYDHCSGTSQAAAFVAGVAARILATHPEIKNVCPKEKTELVRALLRTNALPLGITGSGAGLCRLGGNTTSPLIIQ
>Mature_398_residues
TVDAITIVVRDSVTGDPVSGAMVVLNGDDHSLTNSDGVARFRQPCSRSDGKFHEFLIFRGMEFKPFLLSVSHTQLVGIRT
FPVLLEGQLPPPAMGIGWGLQAIGADVSPLPSFDGIKVGILDTGIDSSHPGLNVIDGFNQGNDAYPFSRPISPHGTFTAG
LIGGKRRGDQGIVGVAPGVDLLDIRVFDPAGIGATRDVICQGVRWAISRGAQILYVGLGSETYSEMEERVWREAYDAGIV
VVAPAGNTMSDHKEVLYPARYVSTICVAACGRNTDVPPDEQPRLSKDGAWFLPSFSRRGPEVDVVAPGVAVCSIVPGQPG
GKTLYDHCSGTSQAAAFVAGVAARILATHPEIKNVCPKEKTELVRALLRTNALPLGITGSGAGLCRLGGNTTSPLIIQ

Specific function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Subtilisin NAT also has fibrinolytic activity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR009020
- InterPro:   IPR010259 [H]

Pfam domain/function: PF05922 Inhibitor_I9; PF00082 Peptidase_S8 [H]

EC number: =3.4.21.62 [H]

Molecular weight: Translated: 42066; Mature: 41934

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVDAITIVVRDSVTGDPVSGAMVVLNGDDHSLTNSDGVARFRQPCSRSDGKFHEFLIFR
CCCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHCCCCCCCCEEEEEEEC
GMEFKPFLLSVSHTQLVGIRTFPVLLEGQLPPPAMGIGWGLQAIGADVSPLPSFDGIKVG
CCCCCCEEEEECCCEEEEEEEEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEE
ILDTGIDSSHPGLNVIDGFNQGNDAYPFSRPISPHGTFTAGLIGGKRRGDQGIVGVAPGV
EEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEECCCC
DLLDIRVFDPAGIGATRDVICQGVRWAISRGAQILYVGLGSETYSEMEERVWREAYDAGI
EEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCCCE
VVVAPAGNTMSDHKEVLYPARYVSTICVAACGRNTDVPPDEQPRLSKDGAWFLPSFSRRG
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCHHCCC
PEVDVVAPGVAVCSIVPGQPGGKTLYDHCSGTSQAAAFVAGVAARILATHPEIKNVCPKE
CEEEEECCCEEEEEECCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCHH
KTELVRALLRTNALPLGITGSGAGLCRLGGNTTSPLIIQ
HHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCCEEEC
>Mature Secondary Structure 
TVDAITIVVRDSVTGDPVSGAMVVLNGDDHSLTNSDGVARFRQPCSRSDGKFHEFLIFR
CCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHCCCCCCCCEEEEEEEC
GMEFKPFLLSVSHTQLVGIRTFPVLLEGQLPPPAMGIGWGLQAIGADVSPLPSFDGIKVG
CCCCCCEEEEECCCEEEEEEEEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEE
ILDTGIDSSHPGLNVIDGFNQGNDAYPFSRPISPHGTFTAGLIGGKRRGDQGIVGVAPGV
EEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEECCCC
DLLDIRVFDPAGIGATRDVICQGVRWAISRGAQILYVGLGSETYSEMEERVWREAYDAGI
EEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCCCE
VVVAPAGNTMSDHKEVLYPARYVSTICVAACGRNTDVPPDEQPRLSKDGAWFLPSFSRRG
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCHHCCC
PEVDVVAPGVAVCSIVPGQPGGKTLYDHCSGTSQAAAFVAGVAARILATHPEIKNVCPKE
CEEEEECCCEEEEEECCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCHH
KTELVRALLRTNALPLGITGSGAGLCRLGGNTTSPLIIQ
HHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1369081 [H]