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The map label for this gene is murD [H]

Identifier: 194335053

GI number: 194335053

Start: 2443226

End: 2444635

Strand: Reverse

Name: murD [H]

Synonym: Paes_2265

Alternate gene names: 194335053

Gene position: 2444635-2443226 (Counterclockwise)

Preceding gene: 194335054

Following gene: 194335052

Centisome position: 97.28

GC content: 56.67

Gene sequence:

>1410_bases
ATGCATACTGCTCCGCTACAGATCGAGGGTGCCAGGGTAAGCGTCATCGGCGCCCGGCGCAGCGGCATCGCAGCTGCTCT
GCTCATGCGTGAACAGGGTGCGTCGGTGTTTGTCAGCGAGCGGGGCGCTCTCGTGCCCGGCGATAGGGAGTTGCTTGCTG
AAAAAGGGATCGATGCCGAGCAGGGCGGACATACCGGGAGGGTGTATGATGCTGATTTCTGTATCGTCAGTCCGGGAGTG
CCGCTTACCGCTCCGGTGATGCAGTTTCTGCGCGCACGCGATATCGCCGTTTACAGCGAGATCGAGGCGGCTTCATGGTT
TTGCCCTGCACGCATCATTGCCGTGACCGGTACGGATGGAAAGACAACAACGGCAACGTTGATCGCGGCTATCTGTGCCG
ACCACGCTCTTGCAGCAGGGTATCGTGTTTTCAGCGTCGGCAATATCGGCGTTCCCTTTTCTTCCATGGTCGGCTCGATG
GCTTCCGGGGACATCGCCGTCGTTGAAGTGAGCAGCTACCAGCTCGAAGGGTGCACCTGGTTTCGGCCGGATATCGCTTT
GATGACCAATATTACTCCGGACCATCTCGATCGTTATGACGGCAGCATGAGGCTCTATGCAGAAGCTAAATACAGAATTT
TTTCACGCCAGAGGCAAGACGATACCCTGATTGTGAACGCTGATGATCCGATACTCCGACAACGGTTCGAAGAACGGCTG
GGCGTTGTGCCTCAACTGGCGGCGTTCGGTCTGGATGCCGGGCGTACCCGGGCGTTTGCCGCCTCAGGAGCGGTGACAGA
AGGCGGATGGATCACTCTGTTCAAGTCTGGAAGCCTTGATGAGCGGGTTATTGCTCATGAGGATCTCCTCAAGCGCGGGT
TCATGGGGAGGCATAATCTTTCAAATGCGCTTGCTGCAGCGCTGGCCGCTGACGCTGCCGGGGTTCCGCTCTCGTCGATA
AAACGGGCGCTGAAGGCTTTCGGGGGCGTCGAGCATCGTCAGGAGTATGTGTGCGAAATTGATGGGGTTATCTGTATCAA
CGATTCCAAAGCCACCAATCTCAATGCAATGCGTCAGGCTCTGGAAGCGGTGAGCGGACCCGTTGTGCTTATTGCCGGAG
GCCGAGACAAAGATGGCGACTACCGTGAACTTCGTGATCTTGTCGCCTCGAAGGTTGAGATGCTTGTTGCTCTGGGGGAG
GCGGCAGATCGTTTTACCGGTGCATACGGCGAAAGTGTTCCTGCTGTTACGGCCGGATCGCTTCGGGAGGCGGTTCGGCT
GGGGCTTCAGCATGCCCGGCGTGGTTCGACACTGCTTTTTTCCCCTGGCTGTTCGAGTTTTGATATGTTTGATAATTTTG
AGCATCGTGGAGCGATGTTTAAATCAATTCTCAGGGAGTCGACCCTATGA

Upstream 100 bases:

>100_bases
TTCAGCTCAAAGGATGGGCTGAGGAGAAAATCGTCATTCGTTTCTGGATCATCACCCTGTTGTTTTTTCTCACCAGTCTT
ATGACGCTGAAACTGCGCTG

Downstream 100 bases:

>100_bases
GCCACAGCGATACTGCATATGGCGCAGGGCATCTGCCGCTTCAGGGTGGAGCAGAGGAGCCGCTGATCGGGAGAAATATC
GCGGGAAAACTGCTGCTGTT

Product: UDP-N-acetylmuramoylalanine/D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MHTAPLQIEGARVSVIGARRSGIAAALLMREQGASVFVSERGALVPGDRELLAEKGIDAEQGGHTGRVYDADFCIVSPGV
PLTAPVMQFLRARDIAVYSEIEAASWFCPARIIAVTGTDGKTTTATLIAAICADHALAAGYRVFSVGNIGVPFSSMVGSM
ASGDIAVVEVSSYQLEGCTWFRPDIALMTNITPDHLDRYDGSMRLYAEAKYRIFSRQRQDDTLIVNADDPILRQRFEERL
GVVPQLAAFGLDAGRTRAFAASGAVTEGGWITLFKSGSLDERVIAHEDLLKRGFMGRHNLSNALAAALAADAAGVPLSSI
KRALKAFGGVEHRQEYVCEIDGVICINDSKATNLNAMRQALEAVSGPVVLIAGGRDKDGDYRELRDLVASKVEMLVALGE
AADRFTGAYGESVPAVTAGSLREAVRLGLQHARRGSTLLFSPGCSSFDMFDNFEHRGAMFKSILRESTL

Sequences:

>Translated_469_residues
MHTAPLQIEGARVSVIGARRSGIAAALLMREQGASVFVSERGALVPGDRELLAEKGIDAEQGGHTGRVYDADFCIVSPGV
PLTAPVMQFLRARDIAVYSEIEAASWFCPARIIAVTGTDGKTTTATLIAAICADHALAAGYRVFSVGNIGVPFSSMVGSM
ASGDIAVVEVSSYQLEGCTWFRPDIALMTNITPDHLDRYDGSMRLYAEAKYRIFSRQRQDDTLIVNADDPILRQRFEERL
GVVPQLAAFGLDAGRTRAFAASGAVTEGGWITLFKSGSLDERVIAHEDLLKRGFMGRHNLSNALAAALAADAAGVPLSSI
KRALKAFGGVEHRQEYVCEIDGVICINDSKATNLNAMRQALEAVSGPVVLIAGGRDKDGDYRELRDLVASKVEMLVALGE
AADRFTGAYGESVPAVTAGSLREAVRLGLQHARRGSTLLFSPGCSSFDMFDNFEHRGAMFKSILRESTL
>Mature_469_residues
MHTAPLQIEGARVSVIGARRSGIAAALLMREQGASVFVSERGALVPGDRELLAEKGIDAEQGGHTGRVYDADFCIVSPGV
PLTAPVMQFLRARDIAVYSEIEAASWFCPARIIAVTGTDGKTTTATLIAAICADHALAAGYRVFSVGNIGVPFSSMVGSM
ASGDIAVVEVSSYQLEGCTWFRPDIALMTNITPDHLDRYDGSMRLYAEAKYRIFSRQRQDDTLIVNADDPILRQRFEERL
GVVPQLAAFGLDAGRTRAFAASGAVTEGGWITLFKSGSLDERVIAHEDLLKRGFMGRHNLSNALAAALAADAAGVPLSSI
KRALKAFGGVEHRQEYVCEIDGVICINDSKATNLNAMRQALEAVSGPVVLIAGGRDKDGDYRELRDLVASKVEMLVALGE
AADRFTGAYGESVPAVTAGSLREAVRLGLQHARRGSTLLFSPGCSSFDMFDNFEHRGAMFKSILRESTL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=465, Percent_Identity=30.752688172043, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI1786274, Length=317, Percent_Identity=28.391167192429, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 50250; Mature: 50250

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTAPLQIEGARVSVIGARRSGIAAALLMREQGASVFVSERGALVPGDRELLAEKGIDAE
CCCCCEEECCCEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHCCCCHH
QGGHTGRVYDADFCIVSPGVPLTAPVMQFLRARDIAVYSEIEAASWFCPARIIAVTGTDG
CCCCCCCEEECCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEECCCC
KTTTATLIAAICADHALAAGYRVFSVGNIGVPFSSMVGSMASGDIAVVEVSSYQLEGCTW
CHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHCCCCCCCEEEEEECCEEECCEEE
FRPDIALMTNITPDHLDRYDGSMRLYAEAKYRIFSRQRQDDTLIVNADDPILRQRFEERL
ECCCEEEEECCCHHHHHHCCCCEEEEEEHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHH
GVVPQLAAFGLDAGRTRAFAASGAVTEGGWITLFKSGSLDERVIAHEDLLKRGFMGRHNL
CCCHHHHHHCCCCCCCEEEEECCCEECCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCH
SNALAAALAADAAGVPLSSIKRALKAFGGVEHRQEYVCEIDGVICINDSKATNLNAMRQA
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHEEECCEEEECCCCCCCHHHHHHH
LEAVSGPVVLIAGGRDKDGDYRELRDLVASKVEMLVALGEAADRFTGAYGESVPAVTAGS
HHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
LREAVRLGLQHARRGSTLLFSPGCSSFDMFDNFEHRGAMFKSILRESTL
HHHHHHHHHHHHCCCCEEEECCCCCCCCHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHTAPLQIEGARVSVIGARRSGIAAALLMREQGASVFVSERGALVPGDRELLAEKGIDAE
CCCCCEEECCCEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHCCCCHH
QGGHTGRVYDADFCIVSPGVPLTAPVMQFLRARDIAVYSEIEAASWFCPARIIAVTGTDG
CCCCCCCEEECCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEECCCC
KTTTATLIAAICADHALAAGYRVFSVGNIGVPFSSMVGSMASGDIAVVEVSSYQLEGCTW
CHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHCCCCCCCEEEEEECCEEECCEEE
FRPDIALMTNITPDHLDRYDGSMRLYAEAKYRIFSRQRQDDTLIVNADDPILRQRFEERL
ECCCEEEEECCCHHHHHHCCCCEEEEEEHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHH
GVVPQLAAFGLDAGRTRAFAASGAVTEGGWITLFKSGSLDERVIAHEDLLKRGFMGRHNL
CCCHHHHHHCCCCCCCEEEEECCCEECCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCH
SNALAAALAADAAGVPLSSIKRALKAFGGVEHRQEYVCEIDGVICINDSKATNLNAMRQA
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHEEECCEEEECCCCCCCHHHHHHH
LEAVSGPVVLIAGGRDKDGDYRELRDLVASKVEMLVALGEAADRFTGAYGESVPAVTAGS
HHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
LREAVRLGLQHARRGSTLLFSPGCSSFDMFDNFEHRGAMFKSILRESTL
HHHHHHHHHHHHCCCCEEEECCCCCCCCHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA