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The map label for this gene is bioD [H]

Identifier: 194334975

GI number: 194334975

Start: 2359335

End: 2360018

Strand: Reverse

Name: bioD [H]

Synonym: Paes_2186

Alternate gene names: 194334975

Gene position: 2360018-2359335 (Counterclockwise)

Preceding gene: 194334976

Following gene: 194334974

Centisome position: 93.92

GC content: 54.24

Gene sequence:

>684_bases
ATGATCATAGCGGTCAGTGGTATCGATACAGGGATAGGAAAGACCATCGCGACAGGCTTGCTTGCCAGGGCTCTGCTGCT
GAAGGGAAGGAGTGTGATAACGCAAAAGGCTGTCCAGACCGGATGCGAGGCACTATCGGACGATATTGTGGTGCATAGGG
AGCTGATGGGGATTGGTTTGCAGGAGGTTGATCGAGAATCGCTGACCTGTCCACTGGTTTTTGCCTATCCGGCCTCTCCT
CACCTTGCGGCAGCAATGGAGCATCGCCGTATAGATCCGGAGGTGATTGCAAAAGCATCGAAAGCGCTGCAGCGCCACTA
TCAGATTATTCTGCTTGAAGGGGCGGGCGGACTTATGGTGCCGCTCAATGACGAGCTGCTGTTTGTCGATTACCTGGCAA
GGGAGGCTGTTCCGCTTATTCTTGTGACCTCTTCGCGACTTGGCAGCATCAATCATACGCTTCTCTCGCTGGAGGCGTGC
CGCCATAGAAAGATTGTGCTCAGGGGTCTTTTGTATAATCGATTCGGCGGGGAAGAGGATGCCGCGATTGCTGTCGATAC
CCGTCGTTTTCTGGAACGGAGCCTTTCCCTGTATGGCTATCGATGTCCTGTCGTCGATCTGCCGGATGCTGCATCGGATT
TGGGCAAATCAGGACTTGAGGCTCTTGCCGCCCTTGTTGGGTGA

Upstream 100 bases:

>100_bases
TCAGGAAATGCCCTGCTTCGGCTGGCGTTGAAGCGACGTGCTGCTGATGTCGCCATGCCGGGTTCAGAAATTCAATAAAT
CAACAGTTCAACATGTTTCA

Downstream 100 bases:

>100_bases
TGTGCGCCGGGGAATCGGGTTGAACACAACTGCTGAAAAAAGAGTGCTGATATGAAGGGACACATTGATACAGATTTTGA
CAGGGGGCATATCTGGCACC

Product: dethiobiotin synthase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MIIAVSGIDTGIGKTIATGLLARALLLKGRSVITQKAVQTGCEALSDDIVVHRELMGIGLQEVDRESLTCPLVFAYPASP
HLAAAMEHRRIDPEVIAKASKALQRHYQIILLEGAGGLMVPLNDELLFVDYLAREAVPLILVTSSRLGSINHTLLSLEAC
RHRKIVLRGLLYNRFGGEEDAAIAVDTRRFLERSLSLYGYRCPVVDLPDAASDLGKSGLEALAALVG

Sequences:

>Translated_227_residues
MIIAVSGIDTGIGKTIATGLLARALLLKGRSVITQKAVQTGCEALSDDIVVHRELMGIGLQEVDRESLTCPLVFAYPASP
HLAAAMEHRRIDPEVIAKASKALQRHYQIILLEGAGGLMVPLNDELLFVDYLAREAVPLILVTSSRLGSINHTLLSLEAC
RHRKIVLRGLLYNRFGGEEDAAIAVDTRRFLERSLSLYGYRCPVVDLPDAASDLGKSGLEALAALVG
>Mature_227_residues
MIIAVSGIDTGIGKTIATGLLARALLLKGRSVITQKAVQTGCEALSDDIVVHRELMGIGLQEVDRESLTCPLVFAYPASP
HLAAAMEHRRIDPEVIAKASKALQRHYQIILLEGAGGLMVPLNDELLFVDYLAREAVPLILVTSSRLGSINHTLLSLEAC
RHRKIVLRGLLYNRFGGEEDAAIAVDTRRFLERSLSLYGYRCPVVDLPDAASDLGKSGLEALAALVG

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=174, Percent_Identity=33.9080459770115, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 24478; Mature: 24478

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIAVSGIDTGIGKTIATGLLARALLLKGRSVITQKAVQTGCEALSDDIVVHRELMGIGL
CEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
QEVDRESLTCPLVFAYPASPHLAAAMEHRRIDPEVIAKASKALQRHYQIILLEGAGGLMV
HHHCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHCEEEEEEECCCCEEE
PLNDELLFVDYLAREAVPLILVTSSRLGSINHTLLSLEACRHRKIVLRGLLYNRFGGEED
ECCCCEEHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AAIAVDTRRFLERSLSLYGYRCPVVDLPDAASDLGKSGLEALAALVG
CEEEHHHHHHHHHHHHHCCEECCEEECCCHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MIIAVSGIDTGIGKTIATGLLARALLLKGRSVITQKAVQTGCEALSDDIVVHRELMGIGL
CEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
QEVDRESLTCPLVFAYPASPHLAAAMEHRRIDPEVIAKASKALQRHYQIILLEGAGGLMV
HHHCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHCEEEEEEECCCCEEE
PLNDELLFVDYLAREAVPLILVTSSRLGSINHTLLSLEACRHRKIVLRGLLYNRFGGEED
ECCCCEEHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AAIAVDTRRFLERSLSLYGYRCPVVDLPDAASDLGKSGLEALAALVG
CEEEHHHHHHHHHHHHHCCEECCEEECCCHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]