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The map label for this gene is nqrF [H]

Identifier: 194334924

GI number: 194334924

Start: 2309066

End: 2310295

Strand: Reverse

Name: nqrF [H]

Synonym: Paes_2133

Alternate gene names: 194334924

Gene position: 2310295-2309066 (Counterclockwise)

Preceding gene: 194334925

Following gene: 194334920

Centisome position: 91.94

GC content: 50.0

Gene sequence:

>1230_bases
ATGCTTGTCACTCTATCGGCTGTCCTTGTCTTTGTCGGCGTGATCGTTCTGCTGGTCTATCTGCTTAACATCGCTGCCCG
AAAACTTCTCCCGCAGGGCCGGGTGACCATTTCCATCAACGATGACGATGACAAAGAATTTTCGGTCACTCCCGGAAGGA
CTCTGCTGTCGACCTTGTCTGATGAGAAGATTTTCATTCCTTCAGCATGCGGCGGCGGCGGGACCTGCGGTATGTGCAAA
TGCAAAATTACCGAAGGTGGAGGACAGGTGCTTCCGACAGAACTGAACCATGTGTCGCGCGCTGAGGTCAAGGAAGATGT
TCGTCTCTCATGTCAGGTCAAGGTTCGGGAGAGCATGAAGGTCTATCTGCCGGAAGAGATCTTTTCTATCCGGCAATGGG
AGTGCACGGTTCTTTCAAACAACAATGTCGCTACCTTTATCAAGGAGCTTGTGCTTGAACTTCCTTCTGATGAAACGCTT
GGTTTCAAAGCGGGAGGCTATATACAGATCGATATTCCCGCCTATCGCCAGATACCCTTTTCAGATTTTGATATCGGCAG
CGAGTACCGGTCTGACTGGGATCATTTCAATATGTGGAATCTCAATGCCACGAATGGAGACTCGACCTTTCGCGCATATT
CCATGGCCAGCCATCCTGCAGAGGGCAACGTTGTCAAGCTGAATGTCCGCATTGCCACGCCACCGCCGAAATTATTCGAC
AGCGCACCTCCAGGGGTGGGATCATCCTATATTTTCAATCTGAAGCCCGGAGACAAGGTCCGCATTTCCGGTCCCTATGG
TGAGTTTTTTATCAAGGATACTGATCGGGAAATGGTCTATATCGGTGGCGGAGCCGGTATGGCGCCTATGCGTTCTCATC
TGTTTCATCTTTTCAGGACCCTGAAAACAACAAGAAAGGTATCGTTCTGGTATGGTGCGCGTTCCCGCAAAGAGATGTTC
TACGATGATGAGTTTCTTGCCATAGCTTCGGAGTTTCCCAATTTCAGCTATAATGTGGCGCTTTCAGACCCGCAGCCCGA
GGACAACTGGGAAGGGCCGACCGGGTTTATTCACAATGTGCTCTATGAGAACTATCTGAAAAACCATGAGGAGCCGGAAG
AGATTGAATACTATATGTGCGGTCCTCCGGTGATGATTTCCTCCGTTGAAAAGATGCTCTACAATCTCGGCGTTGAGAAG
GAGATGATCGCCTACGACGAGTTCTCGTGA

Upstream 100 bases:

>100_bases
GGGGCTTCGCGGTCTGGGGATCGCCATGCTGATTACCGGTCTTATCTCGATGGCGTATATGTCGTTTTCGGGAATAAACT
TGTAACGTTTCGAACAACTT

Downstream 100 bases:

>100_bases
CTGGTGTGGAGTTCTCGAGTGCCCGGTGCTGAGATTTGGTGCAGGGCGGGATGTGATTGTTCCTACCGTATGGCTTTGTA
TTCGCTAATCCTGTGCCGGA

Product: Na(+)-translocating NADH-quinone reductase subunit F

Products: @TETR01txt*tetrahydropteridine!; NO3-; NAD(P); Proton [C]

Alternate protein names: Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MLVTLSAVLVFVGVIVLLVYLLNIAARKLLPQGRVTISINDDDDKEFSVTPGRTLLSTLSDEKIFIPSACGGGGTCGMCK
CKITEGGGQVLPTELNHVSRAEVKEDVRLSCQVKVRESMKVYLPEEIFSIRQWECTVLSNNNVATFIKELVLELPSDETL
GFKAGGYIQIDIPAYRQIPFSDFDIGSEYRSDWDHFNMWNLNATNGDSTFRAYSMASHPAEGNVVKLNVRIATPPPKLFD
SAPPGVGSSYIFNLKPGDKVRISGPYGEFFIKDTDREMVYIGGGAGMAPMRSHLFHLFRTLKTTRKVSFWYGARSRKEMF
YDDEFLAIASEFPNFSYNVALSDPQPEDNWEGPTGFIHNVLYENYLKNHEEPEEIEYYMCGPPVMISSVEKMLYNLGVEK
EMIAYDEFS

Sequences:

>Translated_409_residues
MLVTLSAVLVFVGVIVLLVYLLNIAARKLLPQGRVTISINDDDDKEFSVTPGRTLLSTLSDEKIFIPSACGGGGTCGMCK
CKITEGGGQVLPTELNHVSRAEVKEDVRLSCQVKVRESMKVYLPEEIFSIRQWECTVLSNNNVATFIKELVLELPSDETL
GFKAGGYIQIDIPAYRQIPFSDFDIGSEYRSDWDHFNMWNLNATNGDSTFRAYSMASHPAEGNVVKLNVRIATPPPKLFD
SAPPGVGSSYIFNLKPGDKVRISGPYGEFFIKDTDREMVYIGGGAGMAPMRSHLFHLFRTLKTTRKVSFWYGARSRKEMF
YDDEFLAIASEFPNFSYNVALSDPQPEDNWEGPTGFIHNVLYENYLKNHEEPEEIEYYMCGPPVMISSVEKMLYNLGVEK
EMIAYDEFS
>Mature_409_residues
MLVTLSAVLVFVGVIVLLVYLLNIAARKLLPQGRVTISINDDDDKEFSVTPGRTLLSTLSDEKIFIPSACGGGGTCGMCK
CKITEGGGQVLPTELNHVSRAEVKEDVRLSCQVKVRESMKVYLPEEIFSIRQWECTVLSNNNVATFIKELVLELPSDETL
GFKAGGYIQIDIPAYRQIPFSDFDIGSEYRSDWDHFNMWNLNATNGDSTFRAYSMASHPAEGNVVKLNVRIATPPPKLFD
SAPPGVGSSYIFNLKPGDKVRISGPYGEFFIKDTDREMVYIGGGAGMAPMRSHLFHLFRTLKTTRKVSFWYGARSRKEMF
YDDEFLAIASEFPNFSYNVALSDPQPEDNWEGPTGFIHNVLYENYLKNHEEPEEIEYYMCGPPVMISSVEKMLYNLGVEK
EMIAYDEFS

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. The first step is catalyzed by nqrF, which accepts electrons from NADH and reduces

COG id: COG2871

COG function: function code C; Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrF

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322146, Length=165, Percent_Identity=29.0909090909091, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR010205
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.34 [C]

Molecular weight: Translated: 45967; Mature: 45967

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVTLSAVLVFVGVIVLLVYLLNIAARKLLPQGRVTISINDDDDKEFSVTPGRTLLSTLS
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCHHHHHHCC
DEKIFIPSACGGGGTCGMCKCKITEGGGQVLPTELNHVSRAEVKEDVRLSCQVKVRESMK
CCEEEEECCCCCCCCCCEEEEEEECCCCEECCHHHCHHHHHHHHHCCEEEEEEEEECCEE
VYLPEEIFSIRQWECTVLSNNNVATFIKELVLELPSDETLGFKAGGYIQIDIPAYRQIPF
EECCHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCEECCCEEEEECCHHHCCCC
SDFDIGSEYRSDWDHFNMWNLNATNGDSTFRAYSMASHPAEGNVVKLNVRIATPPPKLFD
CCCCCCCHHHCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCEEEEEEEEECCCHHHHC
SAPPGVGSSYIFNLKPGDKVRISGPYGEFFIKDTDREMVYIGGGAGMAPMRSHLFHLFRT
CCCCCCCCCEEEEECCCCEEEEECCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHH
LKTTRKVSFWYGARSRKEMFYDDEFLAIASEFPNFSYNVALSDPQPEDNWEGPTGFIHNV
HHHHHEEEEEECCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHH
LYENYLKNHEEPEEIEYYMCGPPVMISSVEKMLYNLGVEKEMIAYDEFS
HHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHEEECCCCC
>Mature Secondary Structure
MLVTLSAVLVFVGVIVLLVYLLNIAARKLLPQGRVTISINDDDDKEFSVTPGRTLLSTLS
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCHHHHHHCC
DEKIFIPSACGGGGTCGMCKCKITEGGGQVLPTELNHVSRAEVKEDVRLSCQVKVRESMK
CCEEEEECCCCCCCCCCEEEEEEECCCCEECCHHHCHHHHHHHHHCCEEEEEEEEECCEE
VYLPEEIFSIRQWECTVLSNNNVATFIKELVLELPSDETLGFKAGGYIQIDIPAYRQIPF
EECCHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCEECCCEEEEECCHHHCCCC
SDFDIGSEYRSDWDHFNMWNLNATNGDSTFRAYSMASHPAEGNVVKLNVRIATPPPKLFD
CCCCCCCHHHCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCEEEEEEEEECCCHHHHC
SAPPGVGSSYIFNLKPGDKVRISGPYGEFFIKDTDREMVYIGGGAGMAPMRSHLFHLFRT
CCCCCCCCCEEEEECCCCEEEEECCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHH
LKTTRKVSFWYGARSRKEMFYDDEFLAIASEFPNFSYNVALSDPQPEDNWEGPTGFIHNV
HHHHHEEEEEECCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHH
LYENYLKNHEEPEEIEYYMCGPPVMISSVEKMLYNLGVEKEMIAYDEFS
HHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dihydropteridine; 2 nitric oxide; O2; NAD(P)H [C]

Specific reaction: NAD(P)H + dihydropteridine = NAD(P)+ @TETR01.txt*tetrahydropteridine! 2 nitric oxide + 2 O2 + NAD(P)H = 2 NO3- + NAD(P) + Proton [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA