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The map label for this gene is lpxH [H]

Identifier: 194334795

GI number: 194334795

Start: 2194246

End: 2194983

Strand: Reverse

Name: lpxH [H]

Synonym: Paes_1996

Alternate gene names: 194334795

Gene position: 2194983-2194246 (Counterclockwise)

Preceding gene: 194334796

Following gene: 194334794

Centisome position: 87.35

GC content: 49.19

Gene sequence:

>738_bases
ATGTCAAAAAGCTTTTTTATCAGCGACATTCATCTCGGGCTTCAGGATGAAGGCGCCGAAAAAGAGAAAATCAGGCATCT
GGAAAAGCTGTTCACCATGGTTGCTTCAGATGGCGCAGCCCTCTATATGGTGGGTGATATTCTTGATTACTGGATGGAGT
TCCGCCATGTTATTCCCAGATATTTCTCTTCATTCCTCTGCCTGCTGCAAGAACTTTCGCGCCATGATGTCCGACTCTAC
TGGTTTTCAGGGAACCACGATTTTACACTCGGCCGCTTTTTTACTGACGAGTATGGTGTCAGGTGTCTCTACGGGATGCA
CGAGATGCTCATTGACGAGCGCAGGTTTGTAATCGGTCATGGTGACGGGCTGGACCGCAGCGATATCGGCTACCGTCTTT
TTGTCGCGATGATCCGCAACCGTTTCAATCAGCGGCTGCTTTCGTCGCTGCAGCCGGATCTGGCCATAGCCATTATGCGT
CGATTCTCCCGCATGAGTCGTGATCGCAACCCCGGAGAGAATGGATACGAATCCGATTTTTTGCAGCAATATGCCGATGC
GCTGGTCAGGCAAAAAGATTTTGATTATTTTGTCTGCGGTCACAGCCATGCGCTGAAAAAAAAACTGCTCGCCAACGCGC
GCAGCCACTATATCAATCTTGGTACCTGGATACACGGAAGCTATCCCTACGCGATCTTTGACAATGGGGTTTTTTCCCTG
CAGGAACTCTCAATTTAA

Upstream 100 bases:

>100_bases
GGCATGAGCCCGAAAGAGGTTGATAATATTCTTATACCCGTGGCCGTCGTCTTTCTGGCGATGTTCGTGTGAGCAACCAG
CCATAGATCTGCGCTCAACC

Downstream 100 bases:

>100_bases
ATACGAGAACATCTCAACATGAGTGCATCGAAACAGCTATTGAAACTGGGTTTACCCAAAGGAAGTTTGCAGGATTCCAC
ACTTGATTTGTTTGCAAAAG

Product: metallophosphoesterase

Products: 2,3-bis(3-hydroxymyristoyl)-beta-D-glucosaminyl 1; UMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSKSFFISDIHLGLQDEGAEKEKIRHLEKLFTMVASDGAALYMVGDILDYWMEFRHVIPRYFSSFLCLLQELSRHDVRLY
WFSGNHDFTLGRFFTDEYGVRCLYGMHEMLIDERRFVIGHGDGLDRSDIGYRLFVAMIRNRFNQRLLSSLQPDLAIAIMR
RFSRMSRDRNPGENGYESDFLQQYADALVRQKDFDYFVCGHSHALKKKLLANARSHYINLGTWIHGSYPYAIFDNGVFSL
QELSI

Sequences:

>Translated_245_residues
MSKSFFISDIHLGLQDEGAEKEKIRHLEKLFTMVASDGAALYMVGDILDYWMEFRHVIPRYFSSFLCLLQELSRHDVRLY
WFSGNHDFTLGRFFTDEYGVRCLYGMHEMLIDERRFVIGHGDGLDRSDIGYRLFVAMIRNRFNQRLLSSLQPDLAIAIMR
RFSRMSRDRNPGENGYESDFLQQYADALVRQKDFDYFVCGHSHALKKKLLANARSHYINLGTWIHGSYPYAIFDNGVFSL
QELSI
>Mature_244_residues
SKSFFISDIHLGLQDEGAEKEKIRHLEKLFTMVASDGAALYMVGDILDYWMEFRHVIPRYFSSFLCLLQELSRHDVRLYW
FSGNHDFTLGRFFTDEYGVRCLYGMHEMLIDERRFVIGHGDGLDRSDIGYRLFVAMIRNRFNQRLLSSLQPDLAIAIMRR
FSRMSRDRNPGENGYESDFLQQYADALVRQKDFDYFVCGHSHALKKKLLANARSHYINLGTWIHGSYPYAIFDNGVFSLQ
ELSI

Specific function: Catalyzes the hydrolysis of the pyrophosphate bond of UDP-2,3-diacylglucosamine to yield 2,3-diacylglucosamine 1- phosphate (lipid X) and UMP [H]

COG id: COG2908

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lpxH family [H]

Homologues:

Organism=Escherichia coli, GI1786735, Length=222, Percent_Identity=26.1261261261261, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR010138 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 28728; Mature: 28597

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSFFISDIHLGLQDEGAEKEKIRHLEKLFTMVASDGAALYMVGDILDYWMEFRHVIPR
CCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHH
YFSSFLCLLQELSRHDVRLYWFSGNHDFTLGRFFTDEYGVRCLYGMHEMLIDERRFVIGH
HHHHHHHHHHHHCCCCEEEEEEECCCCEEEHHEECHHHCCHHHHHHHHHHHHCCCEEEEC
GDGLDRSDIGYRLFVAMIRNRFNQRLLSSLQPDLAIAIMRRFSRMSRDRNPGENGYESDF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LQQYADALVRQKDFDYFVCGHSHALKKKLLANARSHYINLGTWIHGSYPYAIFDNGVFSL
HHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHEEEECEEEECCCCEEEECCCCCCH
QELSI
HHCCC
>Mature Secondary Structure 
SKSFFISDIHLGLQDEGAEKEKIRHLEKLFTMVASDGAALYMVGDILDYWMEFRHVIPR
CCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHH
YFSSFLCLLQELSRHDVRLYWFSGNHDFTLGRFFTDEYGVRCLYGMHEMLIDERRFVIGH
HHHHHHHHHHHHCCCCEEEEEEECCCCEEEHHEECHHHCCHHHHHHHHHHHHCCCEEEEC
GDGLDRSDIGYRLFVAMIRNRFNQRLLSSLQPDLAIAIMRRFSRMSRDRNPGENGYESDF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LQQYADALVRQKDFDYFVCGHSHALKKKLLANARSHYINLGTWIHGSYPYAIFDNGVFSL
HHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHEEEECEEEECCCCEEEECCCCCCH
QELSI
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; UDP-2,3-bis(3-hydroxymyristoyl)glucosamine [C]

Specific reaction: H2O + UDP-2,3-bis(3-hydroxymyristoyl)glucosamine = 2,3-bis(3-hydroxymyristoyl)-beta-D-glucosaminyl 1 + UMP [C]

General reaction: Lipid A biosynthesis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA