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The map label for this gene is nemA [H]

Identifier: 194334682

GI number: 194334682

Start: 2057050

End: 2058129

Strand: Reverse

Name: nemA [H]

Synonym: Paes_1883

Alternate gene names: 194334682

Gene position: 2058129-2057050 (Counterclockwise)

Preceding gene: 194334683

Following gene: 194334674

Centisome position: 81.9

GC content: 54.17

Gene sequence:

>1080_bases
ATGACTATGCTTTTTTCCCCTGCCTCTCTTGGAACGCTTACCCTGCAGAACCATATTGTCATGGCGCCCATGACGCGCAG
CCGTGCCACTGGTAATGTCGCCAATGCGCTTATGCGTGAGTATTATGCAGCACGAAGTTCTGCCGGTCTCATCATAACCG
AGGGGACATCTCCCTCGCCAGACGGCCTTGGCTATCCCAGAATTCCAGGTATATTTTCCGGAGCCCAGATCGAAGGATGG
AAATCCATTACGGAGGCGGTGCATCAGCGGGATGGAAAAATTTTCATGCAGCTCATGCATTGCGGTCGAATTGCTCATCC
TCTGAACATGCCTGATGGGGCGGAAATTATCGCCCCTTCCGCAGTGGCTGCTCAGGGGACGATGTATACCGATACGGACG
GATTGCAGCCGTATCCCGTTCCGAGAGCTATGAGCGAGGAAGATATCGCCATCGCAAAAGCCGGGTACGTCGATGCCTCT
CTGCATGCCATGGAGGCCGGTTTCGACGGTGTCGAACTGCATGGCGCCAACGGTTACCTGCTCGAACAGTTTTTCAGGCC
GAATACCAATCTCAGAACAGACTGTTACGGCGGTTCGGTGGAAAATCGGGCCCGTTTCATGCTGGAAGTGCTCGATGAGG
TGATCGCTGCTGTTGGTGACCAACGGGTGGGTATTCGTCTTTCGCCGTTCGGCGTCTTCAATGATATGCCGCTCTATGAG
GCAATGGAGGAAGAGTATCGCTGGCTTGCCGGGGCTATCGCCAGGGTCGCCCCGCTCTACATTCATCTTGTCGATCACTC
CTCTATGGGAGCACCGACGGTGCCTGATTCGATCAAGGAATCATTTCGCAAAAGCTTCAAGGCAACGCTTATTCTTTCCG
GCGGTTATGATGCAGCGCGTGCAGCAAGAGATCTCGATGCCCGAAAGTGCGATCTGGTTGCCGTTGGGAGAGCGTTTATC
GCTAATCCTGATCTCGTCGAACGCTGGCATGCGGGAGCTCCTCTCAATACCCCTGATCCCGATACGTTTTATTCAGCAGG
ACCGAAAGGATACATCGATTATCCGGTTCTGGAGGGGTAG

Upstream 100 bases:

>100_bases
AGCAGCACATACGCTATATTGCAGCATTGACCAGGATTTGCCTGAACAGGCGATAACGGGCTATGCACCATGCCTTGATG
ATGCAAAGGAGGTTTCTCTG

Downstream 100 bases:

>100_bases
TTGTATACAAAAGATATTTCAGCAGGTTATTGCGAGTCGTTTGTAGTAGCCGCGGTCAATTTTTTCAGTTCCGGGCAAGA
GATGCCATGAGGTGATTCTT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MTMLFSPASLGTLTLQNHIVMAPMTRSRATGNVANALMREYYAARSSAGLIITEGTSPSPDGLGYPRIPGIFSGAQIEGW
KSITEAVHQRDGKIFMQLMHCGRIAHPLNMPDGAEIIAPSAVAAQGTMYTDTDGLQPYPVPRAMSEEDIAIAKAGYVDAS
LHAMEAGFDGVELHGANGYLLEQFFRPNTNLRTDCYGGSVENRARFMLEVLDEVIAAVGDQRVGIRLSPFGVFNDMPLYE
AMEEEYRWLAGAIARVAPLYIHLVDHSSMGAPTVPDSIKESFRKSFKATLILSGGYDAARAARDLDARKCDLVAVGRAFI
ANPDLVERWHAGAPLNTPDPDTFYSAGPKGYIDYPVLEG

Sequences:

>Translated_359_residues
MTMLFSPASLGTLTLQNHIVMAPMTRSRATGNVANALMREYYAARSSAGLIITEGTSPSPDGLGYPRIPGIFSGAQIEGW
KSITEAVHQRDGKIFMQLMHCGRIAHPLNMPDGAEIIAPSAVAAQGTMYTDTDGLQPYPVPRAMSEEDIAIAKAGYVDAS
LHAMEAGFDGVELHGANGYLLEQFFRPNTNLRTDCYGGSVENRARFMLEVLDEVIAAVGDQRVGIRLSPFGVFNDMPLYE
AMEEEYRWLAGAIARVAPLYIHLVDHSSMGAPTVPDSIKESFRKSFKATLILSGGYDAARAARDLDARKCDLVAVGRAFI
ANPDLVERWHAGAPLNTPDPDTFYSAGPKGYIDYPVLEG
>Mature_358_residues
TMLFSPASLGTLTLQNHIVMAPMTRSRATGNVANALMREYYAARSSAGLIITEGTSPSPDGLGYPRIPGIFSGAQIEGWK
SITEAVHQRDGKIFMQLMHCGRIAHPLNMPDGAEIIAPSAVAAQGTMYTDTDGLQPYPVPRAMSEEDIAIAKAGYVDASL
HAMEAGFDGVELHGANGYLLEQFFRPNTNLRTDCYGGSVENRARFMLEVLDEVIAAVGDQRVGIRLSPFGVFNDMPLYEA
MEEEYRWLAGAIARVAPLYIHLVDHSSMGAPTVPDSIKESFRKSFKATLILSGGYDAARAARDLDARKCDLVAVGRAFIA
NPDLVERWHAGAPLNTPDPDTFYSAGPKGYIDYPVLEG

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=360, Percent_Identity=41.9444444444444, Blast_Score=258, Evalue=5e-70,
Organism=Escherichia coli, GI1789463, Length=339, Percent_Identity=28.9085545722714, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI72001454, Length=261, Percent_Identity=29.5019157088123, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17559804, Length=267, Percent_Identity=29.2134831460674, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17559802, Length=276, Percent_Identity=30.4347826086957, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17540738, Length=351, Percent_Identity=28.7749287749288, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17565138, Length=301, Percent_Identity=29.9003322259136, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=259, Percent_Identity=28.1853281853282, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321973, Length=378, Percent_Identity=38.3597883597884, Blast_Score=218, Evalue=9e-58,
Organism=Saccharomyces cerevisiae, GI6325086, Length=381, Percent_Identity=36.2204724409449, Blast_Score=206, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38906; Mature: 38774

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMLFSPASLGTLTLQNHIVMAPMTRSRATGNVANALMREYYAARSSAGLIITEGTSPSP
CCEEECCCCCCEEEECCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCC
DGLGYPRIPGIFSGAQIEGWKSITEAVHQRDGKIFMQLMHCGRIAHPLNMPDGAEIIAPS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCEECCC
AVAAQGTMYTDTDGLQPYPVPRAMSEEDIAIAKAGYVDASLHAMEAGFDGVELHGANGYL
HHHHCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCEEEECCCCHH
LEQFFRPNTNLRTDCYGGSVENRARFMLEVLDEVIAAVGDQRVGIRLSPFGVFNDMPLYE
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHH
AMEEEYRWLAGAIARVAPLYIHLVDHSSMGAPTVPDSIKESFRKSFKATLILSGGYDAAR
HHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCHHHHHHHHHCCEEEEEEECCCHHHH
AARDLDARKCDLVAVGRAFIANPDLVERWHAGAPLNTPDPDTFYSAGPKGYIDYPVLEG
HHHHCCCHHCCEEEECHHHHCCCHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TMLFSPASLGTLTLQNHIVMAPMTRSRATGNVANALMREYYAARSSAGLIITEGTSPSP
CEEECCCCCCEEEECCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCC
DGLGYPRIPGIFSGAQIEGWKSITEAVHQRDGKIFMQLMHCGRIAHPLNMPDGAEIIAPS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCEECCC
AVAAQGTMYTDTDGLQPYPVPRAMSEEDIAIAKAGYVDASLHAMEAGFDGVELHGANGYL
HHHHCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCEEEECCCCHH
LEQFFRPNTNLRTDCYGGSVENRARFMLEVLDEVIAAVGDQRVGIRLSPFGVFNDMPLYE
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHH
AMEEEYRWLAGAIARVAPLYIHLVDHSSMGAPTVPDSIKESFRKSFKATLILSGGYDAAR
HHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCHHHHHHHHHCCEEEEEEECCCHHHH
AARDLDARKCDLVAVGRAFIANPDLVERWHAGAPLNTPDPDTFYSAGPKGYIDYPVLEG
HHHHCCCHHCCEEEECHHHHCCCHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]