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The map label for this gene is pcrA [H]

Identifier: 194334487

GI number: 194334487

Start: 1833561

End: 1835792

Strand: Reverse

Name: pcrA [H]

Synonym: Paes_1682

Alternate gene names: 194334487

Gene position: 1835792-1833561 (Counterclockwise)

Preceding gene: 194334488

Following gene: 194334486

Centisome position: 73.05

GC content: 50.27

Gene sequence:

>2232_bases
TTGAGTGATATTCTTCAGCAGTTGAACGATGTGCAGCGCCAGGCCGTTCAGACGACATCCGGGCCGGTTATGGTTCTTGC
CGGTGCCGGGTCCGGCAAGACACGGGTTATTACCTACAGGATCGCCTATCTTATCGAGCAGGAGCGGGTTGAGCCGCACC
AGATACTTGCCTTGACCTTTACCAACAAGGCGGCAAAAGAGATGCGGCACAGAATCGATCAACTGCTCCATCAGGGCAGT
ACCCGGGGATTATGGATCGGGACATTTCACTCTGTTTTTGCCCGTATTCTGCGAAACTATATCCATCTTATCGGCTATAA
CTCCAATTTTTCGATATACGATTCGGATGACAGCAAAAGCCTGGTGAAGCAGGTGATGAAAGAGTTGAATATCGATACTG
ATTCGGTTCCGGTCAATACCATACAGGGTATTATCAGCCGGGCAAAGAACAATTTTATTCTTCCTCCGCAGTTTCAGCGC
GACGCTGCTGATTACAACCAGCAGAAAGCGGCAAAGGTGTATGAGGTGTATACGCGAAAGCTGAAGGAAAACAATGCTCT
TGATTTCGATGATCTTCTCATCAAGCCGCTGGAGTTGTTTGTTGAAAGTCCTCAGCTTCTTGAGCAGCTGCAGGACTATT
TCCGATATATTCTCATAGACGAGTACCAGGACACCAACCGGGCGCAGTACCTTGTTGCCAAACAGTTGGCATCGAAACAC
CGCAATATTTTTGTCGTGGGAGATGATGCCCAGTCAATTTATTCCTGGCGTGGCGCCGATATCACCAACATTCTGAACTT
CCAGCAGGATTACCCCGATTCGTCGGTGTTCAAGCTGGTCGAGAACTATCGCAGTACGCAGACGATTCTCAAGGCTGCCA
ACGGCATCATCAGAAAAAACCGCCGACAGATTGAAAAAGAGCTTGTCTCCCACAAAAGCACCGGCGAGCCTCTGACCCTG
ATCGAATCGTTCAACGAGAAACGCGAGGCGGACAAGGTTGGGGAGTATATCCGTTCCCTGAAATTGTCGAAGGACTACGA
ATACAGGGATTTTGCAGTCTTTTACCGCACCAATGCCCAGTCGAGGGTCCTTGAGGATGTGTTGCGGCTGCATCGTCTTC
CCTACAGAATTTTCGGTAGTGTATCCTTCTATAAGCGCAAGGAGATCAAGGACGCTATAGCGTACCTCCGCTTTATTCTC
AATGACCACGATAACGAGTCGCTGCTGCGCGTTATTAATTTCCCTCCGCGCAAGATTGGAGATGTCAGCATCAACAAACT
GCGTGATTTTGCCGAATCGAGGAACCTGTCGCTCTACGGTGCTATCCGTCATGCTGCCGAGGGGGGCTTTCAGGCAAGGT
TGCTCAACGCACTCAATGCGTTCAGCTCGATTATCGAGCAGCTTAAAGAGATTTCCCGGGAAGGGTCTGTCTATGACGTG
CTCAACTCTTTGTACGAGTTGACAGGGATTCCTTTACTGCTACGGGCTGAAAATACGCCCGAGGCGATGGCGCGGTATGA
AAATCTTCAGGAGTTGCTGTCGATGGCCCGTGATTTTTCAGACCATCATCAGGGGGCAAACGGCTTGAATGACTTTATGA
ACGATATTGCTCTTGCAACGGATTCCGATGAGATACAGGAATCTGATAACTATGTTTCACTCATGACCGTTCACGCAGCC
AAGGGGCTGGAGTTTCCCGTAGTATTTATTACCGGTCTTGAGGAAAAGCTCTTTCCTCTCAATTACTATGAGCAGGAGGA
ACTGGAGGAGGAGAGGCGCTTGTTTTATGTTGCCTTGACCCGCGCTCAGGAGAAACTGTATTTGTCCTGGTCCAAGAGCC
GTTACCATTATGGTCAGCCGCAGGGCTGCATTCGTTCGATGTTTATCGATGAGGTTGATAGCGATATTGTCGAGTCTGAA
GGGGGGCGGCTTCTCTCGGAGTCCCGCATGGAGAGAGAGCGGCCGCGTCAGGGCGTTCAACAGGCCGGGCGTTTCAATGG
CCGTTCTTCCGTTCCGTCATCATTCCATGAGCGACCTCGTGGTGAGGGGGCCGGGGTGAAAAAAACTTCGGACATGAAAG
CCGGAACGAGGGTTCATCATGCTATGTTCGGGCCTGGCGTGGTTATGGCGGTTCAGGGTAGCGGTTCTCGTCAGAAAGCA
CAGATCAAGTTCAGGAATGCCGGCGAAAAAACCCTTATGGTTCAGTACGCTAATCTTACAATTGTTTCATAG

Upstream 100 bases:

>100_bases
AGAGGTCCCCTTGAGTTGTTGAGCTGGTTTGCAGAAACGGAAAATTATACGAAATTCTACAGGTCCGAATTTTCAAACCA
CTATTTTCCATGATTGTACT

Downstream 100 bases:

>100_bases
CTGTCGTTCGTCCGCGAGGTCGTTCGGCCTGTCATAAACATTCTGGAATATGAAAATCCTTTTTGGTGTTCAGGGAACTG
GTAACGGCCATATCAGCCGA

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 743; Mature: 742

Protein sequence:

>743_residues
MSDILQQLNDVQRQAVQTTSGPVMVLAGAGSGKTRVITYRIAYLIEQERVEPHQILALTFTNKAAKEMRHRIDQLLHQGS
TRGLWIGTFHSVFARILRNYIHLIGYNSNFSIYDSDDSKSLVKQVMKELNIDTDSVPVNTIQGIISRAKNNFILPPQFQR
DAADYNQQKAAKVYEVYTRKLKENNALDFDDLLIKPLELFVESPQLLEQLQDYFRYILIDEYQDTNRAQYLVAKQLASKH
RNIFVVGDDAQSIYSWRGADITNILNFQQDYPDSSVFKLVENYRSTQTILKAANGIIRKNRRQIEKELVSHKSTGEPLTL
IESFNEKREADKVGEYIRSLKLSKDYEYRDFAVFYRTNAQSRVLEDVLRLHRLPYRIFGSVSFYKRKEIKDAIAYLRFIL
NDHDNESLLRVINFPPRKIGDVSINKLRDFAESRNLSLYGAIRHAAEGGFQARLLNALNAFSSIIEQLKEISREGSVYDV
LNSLYELTGIPLLLRAENTPEAMARYENLQELLSMARDFSDHHQGANGLNDFMNDIALATDSDEIQESDNYVSLMTVHAA
KGLEFPVVFITGLEEKLFPLNYYEQEELEEERRLFYVALTRAQEKLYLSWSKSRYHYGQPQGCIRSMFIDEVDSDIVESE
GGRLLSESRMERERPRQGVQQAGRFNGRSSVPSSFHERPRGEGAGVKKTSDMKAGTRVHHAMFGPGVVMAVQGSGSRQKA
QIKFRNAGEKTLMVQYANLTIVS

Sequences:

>Translated_743_residues
MSDILQQLNDVQRQAVQTTSGPVMVLAGAGSGKTRVITYRIAYLIEQERVEPHQILALTFTNKAAKEMRHRIDQLLHQGS
TRGLWIGTFHSVFARILRNYIHLIGYNSNFSIYDSDDSKSLVKQVMKELNIDTDSVPVNTIQGIISRAKNNFILPPQFQR
DAADYNQQKAAKVYEVYTRKLKENNALDFDDLLIKPLELFVESPQLLEQLQDYFRYILIDEYQDTNRAQYLVAKQLASKH
RNIFVVGDDAQSIYSWRGADITNILNFQQDYPDSSVFKLVENYRSTQTILKAANGIIRKNRRQIEKELVSHKSTGEPLTL
IESFNEKREADKVGEYIRSLKLSKDYEYRDFAVFYRTNAQSRVLEDVLRLHRLPYRIFGSVSFYKRKEIKDAIAYLRFIL
NDHDNESLLRVINFPPRKIGDVSINKLRDFAESRNLSLYGAIRHAAEGGFQARLLNALNAFSSIIEQLKEISREGSVYDV
LNSLYELTGIPLLLRAENTPEAMARYENLQELLSMARDFSDHHQGANGLNDFMNDIALATDSDEIQESDNYVSLMTVHAA
KGLEFPVVFITGLEEKLFPLNYYEQEELEEERRLFYVALTRAQEKLYLSWSKSRYHYGQPQGCIRSMFIDEVDSDIVESE
GGRLLSESRMERERPRQGVQQAGRFNGRSSVPSSFHERPRGEGAGVKKTSDMKAGTRVHHAMFGPGVVMAVQGSGSRQKA
QIKFRNAGEKTLMVQYANLTIVS
>Mature_742_residues
SDILQQLNDVQRQAVQTTSGPVMVLAGAGSGKTRVITYRIAYLIEQERVEPHQILALTFTNKAAKEMRHRIDQLLHQGST
RGLWIGTFHSVFARILRNYIHLIGYNSNFSIYDSDDSKSLVKQVMKELNIDTDSVPVNTIQGIISRAKNNFILPPQFQRD
AADYNQQKAAKVYEVYTRKLKENNALDFDDLLIKPLELFVESPQLLEQLQDYFRYILIDEYQDTNRAQYLVAKQLASKHR
NIFVVGDDAQSIYSWRGADITNILNFQQDYPDSSVFKLVENYRSTQTILKAANGIIRKNRRQIEKELVSHKSTGEPLTLI
ESFNEKREADKVGEYIRSLKLSKDYEYRDFAVFYRTNAQSRVLEDVLRLHRLPYRIFGSVSFYKRKEIKDAIAYLRFILN
DHDNESLLRVINFPPRKIGDVSINKLRDFAESRNLSLYGAIRHAAEGGFQARLLNALNAFSSIIEQLKEISREGSVYDVL
NSLYELTGIPLLLRAENTPEAMARYENLQELLSMARDFSDHHQGANGLNDFMNDIALATDSDEIQESDNYVSLMTVHAAK
GLEFPVVFITGLEEKLFPLNYYEQEELEEERRLFYVALTRAQEKLYLSWSKSRYHYGQPQGCIRSMFIDEVDSDIVESEG
GRLLSESRMERERPRQGVQQAGRFNGRSSVPSSFHERPRGEGAGVKKTSDMKAGTRVHHAMFGPGVVMAVQGSGSRQKAQ
IKFRNAGEKTLMVQYANLTIVS

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=747, Percent_Identity=37.2155287817938, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI48994965, Length=660, Percent_Identity=37.5757575757576, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1787196, Length=344, Percent_Identity=26.1627906976744, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322369, Length=760, Percent_Identity=27.3684210526316, Blast_Score=231, Evalue=3e-61,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 85170; Mature: 85038

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDILQQLNDVQRQAVQTTSGPVMVLAGAGSGKTRVITYRIAYLIEQERVEPHQILALTF
CHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEHHHHHHHHHCCCCEEEEEEEE
TNKAAKEMRHRIDQLLHQGSTRGLWIGTFHSVFARILRNYIHLIGYNSNFSIYDSDDSKS
CCHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHEECCCCCEEEECCCHHH
LVKQVMKELNIDTDSVPVNTIQGIISRAKNNFILPPQFQRDAADYNQQKAAKVYEVYTRK
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHH
LKENNALDFDDLLIKPLELFVESPQLLEQLQDYFRYILIDEYQDTNRAQYLVAKQLASKH
HHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
RNIFVVGDDAQSIYSWRGADITNILNFQQDYPDSSVFKLVENYRSTQTILKAANGIIRKN
CEEEEEECCHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRQIEKELVSHKSTGEPLTLIESFNEKREADKVGEYIRSLKLSKDYEYRDFAVFYRTNAQ
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCH
SRVLEDVLRLHRLPYRIFGSVSFYKRKEIKDAIAYLRFILNDHDNESLLRVINFPPRKIG
HHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCC
DVSINKLRDFAESRNLSLYGAIRHAAEGGFQARLLNALNAFSSIIEQLKEISREGSVYDV
CCHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LNSLYELTGIPLLLRAENTPEAMARYENLQELLSMARDFSDHHQGANGLNDFMNDIALAT
HHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEECC
DSDEIQESDNYVSLMTVHAAKGLEFPVVFITGLEEKLFPLNYYEQEELEEERRLFYVALT
CCHHHHCCCCEEEEEEEEHHCCCCCCEEEEECCCHHCCCCCCCCHHHHHHHHHEEHHHHH
RAQEKLYLSWSKSRYHYGQPQGCIRSMFIDEVDSDIVESEGGRLLSESRMERERPRQGVQ
HHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
QAGRFNGRSSVPSSFHERPRGEGAGVKKTSDMKAGTRVHHAMFGPGVVMAVQGSGSRQKA
HHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCEE
QIKFRNAGEKTLMVQYANLTIVS
EEEEECCCCEEEEEEEECEEEEC
>Mature Secondary Structure 
SDILQQLNDVQRQAVQTTSGPVMVLAGAGSGKTRVITYRIAYLIEQERVEPHQILALTF
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEHHHHHHHHHCCCCEEEEEEEE
TNKAAKEMRHRIDQLLHQGSTRGLWIGTFHSVFARILRNYIHLIGYNSNFSIYDSDDSKS
CCHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHEECCCCCEEEECCCHHH
LVKQVMKELNIDTDSVPVNTIQGIISRAKNNFILPPQFQRDAADYNQQKAAKVYEVYTRK
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHH
LKENNALDFDDLLIKPLELFVESPQLLEQLQDYFRYILIDEYQDTNRAQYLVAKQLASKH
HHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
RNIFVVGDDAQSIYSWRGADITNILNFQQDYPDSSVFKLVENYRSTQTILKAANGIIRKN
CEEEEEECCHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRQIEKELVSHKSTGEPLTLIESFNEKREADKVGEYIRSLKLSKDYEYRDFAVFYRTNAQ
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCH
SRVLEDVLRLHRLPYRIFGSVSFYKRKEIKDAIAYLRFILNDHDNESLLRVINFPPRKIG
HHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCC
DVSINKLRDFAESRNLSLYGAIRHAAEGGFQARLLNALNAFSSIIEQLKEISREGSVYDV
CCHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LNSLYELTGIPLLLRAENTPEAMARYENLQELLSMARDFSDHHQGANGLNDFMNDIALAT
HHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEECC
DSDEIQESDNYVSLMTVHAAKGLEFPVVFITGLEEKLFPLNYYEQEELEEERRLFYVALT
CCHHHHCCCCEEEEEEEEHHCCCCCCEEEEECCCHHCCCCCCCCHHHHHHHHHEEHHHHH
RAQEKLYLSWSKSRYHYGQPQGCIRSMFIDEVDSDIVESEGGRLLSESRMERERPRQGVQ
HHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
QAGRFNGRSSVPSSFHERPRGEGAGVKKTSDMKAGTRVHHAMFGPGVVMAVQGSGSRQKA
HHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCEE
QIKFRNAGEKTLMVQYANLTIVS
EEEEECCCCEEEEEEEECEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]