Click here to switch to the map view.

The map label for this gene is tpiA

Identifier: 194334367

GI number: 194334367

Start: 1701451

End: 1702209

Strand: Reverse

Name: tpiA

Synonym: Paes_1562

Alternate gene names: 194334367

Gene position: 1702209-1701451 (Counterclockwise)

Preceding gene: 194334368

Following gene: 194334365

Centisome position: 67.74

GC content: 52.04

Gene sequence:

>759_bases
ATGCGAAAAAAAATTGTTGTTGGTAACTGGAAAATGAATAAGACCATCGCTGAGGCGGTTGAGCTTTCGTCTGCTATTAT
TGATCAGCTCGGCGAAGTTTCAGCTTCGTGTGAGGTAGGCATCGCTCCGGCTTTTCCTGCATTGAGCGGTGTACATGGTG
TTATCAGTGGGACCGGCATCCATCTTGCGGCTCAGAACTGTCACTATGAGGATGACGGTGCTTTTACCGGAGAGGTTTCT
GTCCGTATGCTTGATGAGGCAGGATGCAGCTATGTGATCGTCGGACATTCCGAACGTCGGCAGTATTTCGGTGATACTAA
TCCGGTCGTCAATCTCAAAGTCAAAAAAGCGCTCTCTGCTGGCCTGAATGTCATTCTTTGCGTTGGAGAGACTCTGGATG
AGCGTGAAAAGGGGATTACTACAGAGGTGGTGACCTGCCAGGTGAAAGAGGGGCTTGAAGGTGTTGCTGATATCGGGGAT
ATCGTTATTGCCTATGAGCCGGTATGGGCTATTGGTACCGGGAAAACAGCCAGTAGCCTGCAGGCGCAGGAGGTGCATGC
TTCTATCCGCGCGACCGTCGCCGGCCTCTACAGCGAGCCTGCTGCAGCCGGAGTGCGGATCCAGTATGGCGGAAGCGTCA
AACCCTCCAATGCCGAAGAACTTTTTGCCATGCCTGATATTGACGGCGGTCTTATCGGCGGAGCCAGTCTCAACGCTGAA
GACTTTGTCGCTATCGTCAAAGCAGCAGAGAAGAGTTGA

Upstream 100 bases:

>100_bases
AGTCTCGCGTTGAATTAAAGGATGCCCGCAAAGGAAAACACAGCTTTCCGATCGACCAGATAGATAAAATTTATCGTGAC
ATTGTAACCGAATATTGAAC

Downstream 100 bases:

>100_bases
ATTGTTGATCTGAAAAATTGTTTTTCGCCTGGGAACTCTCAAAAAAGGGAACAGCATTGTTGAGTGCTGTTCCCTTTTTT
CGTTGGGTGTGTAACGGGGT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MRKKIVVGNWKMNKTIAEAVELSSAIIDQLGEVSASCEVGIAPAFPALSGVHGVISGTGIHLAAQNCHYEDDGAFTGEVS
VRMLDEAGCSYVIVGHSERRQYFGDTNPVVNLKVKKALSAGLNVILCVGETLDEREKGITTEVVTCQVKEGLEGVADIGD
IVIAYEPVWAIGTGKTASSLQAQEVHASIRATVAGLYSEPAAAGVRIQYGGSVKPSNAEELFAMPDIDGGLIGGASLNAE
DFVAIVKAAEKS

Sequences:

>Translated_252_residues
MRKKIVVGNWKMNKTIAEAVELSSAIIDQLGEVSASCEVGIAPAFPALSGVHGVISGTGIHLAAQNCHYEDDGAFTGEVS
VRMLDEAGCSYVIVGHSERRQYFGDTNPVVNLKVKKALSAGLNVILCVGETLDEREKGITTEVVTCQVKEGLEGVADIGD
IVIAYEPVWAIGTGKTASSLQAQEVHASIRATVAGLYSEPAAAGVRIQYGGSVKPSNAEELFAMPDIDGGLIGGASLNAE
DFVAIVKAAEKS
>Mature_252_residues
MRKKIVVGNWKMNKTIAEAVELSSAIIDQLGEVSASCEVGIAPAFPALSGVHGVISGTGIHLAAQNCHYEDDGAFTGEVS
VRMLDEAGCSYVIVGHSERRQYFGDTNPVVNLKVKKALSAGLNVILCVGETLDEREKGITTEVVTCQVKEGLEGVADIGD
IVIAYEPVWAIGTGKTASSLQAQEVHASIRATVAGLYSEPAAAGVRIQYGGSVKPSNAEELFAMPDIDGGLIGGASLNAE
DFVAIVKAAEKS

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=249, Percent_Identity=45.7831325301205, Blast_Score=198, Evalue=3e-51,
Organism=Homo sapiens, GI4507645, Length=249, Percent_Identity=45.7831325301205, Blast_Score=198, Evalue=4e-51,
Organism=Escherichia coli, GI1790353, Length=252, Percent_Identity=47.6190476190476, Blast_Score=199, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17536593, Length=250, Percent_Identity=46.8, Blast_Score=195, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6320255, Length=251, Percent_Identity=43.0278884462151, Blast_Score=177, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28572008, Length=253, Percent_Identity=48.6166007905138, Blast_Score=205, Evalue=2e-53,
Organism=Drosophila melanogaster, GI28572006, Length=253, Percent_Identity=48.6166007905138, Blast_Score=205, Evalue=2e-53,
Organism=Drosophila melanogaster, GI28572004, Length=253, Percent_Identity=48.6166007905138, Blast_Score=205, Evalue=3e-53,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_PROA2 (B4S9B0)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002016227.1
- ProteinModelPortal:   B4S9B0
- SMR:   B4S9B0
- GeneID:   6459292
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_1562
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26275; Mature: 26275

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 96-96 ACT_SITE 166-166 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKIVVGNWKMNKTIAEAVELSSAIIDQLGEVSASCEVGIAPAFPALSGVHGVISGTGI
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHHCCCCEEECCCE
HLAAQNCHYEDDGAFTGEVSVRMLDEAGCSYVIVGHSERRQYFGDTNPVVNLKVKKALSA
EEEECCCCCCCCCCEECEEEEEEECCCCCCEEEEECCHHHHHCCCCCCEEEEEEHHHHHC
GLNVILCVGETLDEREKGITTEVVTCQVKEGLEGVADIGDIVIAYEPVWAIGTGKTASSL
CCEEEEEECCCHHHHHCCCEEEEEEEEEHHCCHHHHCCCCEEEEECCEEEEECCCCHHHH
QAQEVHASIRATVAGLYSEPAAAGVRIQYGGSVKPSNAEELFAMPDIDGGLIGGASLNAE
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHEEECCCCCCCEECCCCCCHH
DFVAIVKAAEKS
HHHHHHHHHCCC
>Mature Secondary Structure
MRKKIVVGNWKMNKTIAEAVELSSAIIDQLGEVSASCEVGIAPAFPALSGVHGVISGTGI
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHHCCCCEEECCCE
HLAAQNCHYEDDGAFTGEVSVRMLDEAGCSYVIVGHSERRQYFGDTNPVVNLKVKKALSA
EEEECCCCCCCCCCEECEEEEEEECCCCCCEEEEECCHHHHHCCCCCCEEEEEEHHHHHC
GLNVILCVGETLDEREKGITTEVVTCQVKEGLEGVADIGDIVIAYEPVWAIGTGKTASSL
CCEEEEEECCCHHHHHCCCEEEEEEEEEHHCCHHHHCCCCEEEEECCEEEEECCCCHHHH
QAQEVHASIRATVAGLYSEPAAAGVRIQYGGSVKPSNAEELFAMPDIDGGLIGGASLNAE
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHEEECCCCCCCEECCCCCCHH
DFVAIVKAAEKS
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA