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The map label for this gene is dut

Identifier: 194334336

GI number: 194334336

Start: 1669959

End: 1670396

Strand: Reverse

Name: dut

Synonym: Paes_1529

Alternate gene names: 194334336

Gene position: 1670396-1669959 (Counterclockwise)

Preceding gene: 194334338

Following gene: 194334329

Centisome position: 66.47

GC content: 53.88

Gene sequence:

>438_bases
ATGCTAAAAGTAAACATTGTTCGTGTAAACAAAAATGCCATCCTGCCGCGTTATGCGACGCAGCATGCAGCAGGAATGGA
TATTGCCGCATGTCTGGATGTTCCTCTTACCATCCAGCCTTCAGAGACGGTGCTTGTTCCCACCGGCCTTGCAGTGGAGC
TTCCTGAGGGCTATGAGGCTCAGCTCAGGCCTCGCAGTGGTCTTGCTCTTAAATATGCTATTTCGCTTCCTAACGCTCCG
GCTACCATCGATGCCGATTACCGGGGTGAGGTCAAGGTTATTCTGGTGAATCTCGGTCAGGAGCCTTTTTGTGTCAATCA
CGGTGATCGTATCGCTCAGATGGTGATCGCAAGGTATGAGCATGCTGAGCTGCAGGAGGTGGAAGAGCTCAGCAGGACCC
CCAGGGGCAGCGGCGGCTTCGGCCACACAGGGGTATGA

Upstream 100 bases:

>100_bases
GCTCCGAACGCAAGGGCGTAAACCGGATTGGTATGTGCGTTCACAACAGCTATGATTTTCTTGTATATTCTGCCTTCACT
CTTACAAACTGATCTGATCA

Downstream 100 bases:

>100_bases
GAATAAAAAACAGGTCAAAAGTCTACGCTCAGCACGTAACAGAGGAGAAAGGATGCAGGGCTTTGCAATACCGGAACGGC
GCCAGCGGCACCGTATCAGT

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase Dut

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MLKVNIVRVNKNAILPRYATQHAAGMDIAACLDVPLTIQPSETVLVPTGLAVELPEGYEAQLRPRSGLALKYAISLPNAP
ATIDADYRGEVKVILVNLGQEPFCVNHGDRIAQMVIARYEHAELQEVEELSRTPRGSGGFGHTGV

Sequences:

>Translated_145_residues
MLKVNIVRVNKNAILPRYATQHAAGMDIAACLDVPLTIQPSETVLVPTGLAVELPEGYEAQLRPRSGLALKYAISLPNAP
ATIDADYRGEVKVILVNLGQEPFCVNHGDRIAQMVIARYEHAELQEVEELSRTPRGSGGFGHTGV
>Mature_145_residues
MLKVNIVRVNKNAILPRYATQHAAGMDIAACLDVPLTIQPSETVLVPTGLAVELPEGYEAQLRPRSGLALKYAISLPNAP
ATIDADYRGEVKVILVNLGQEPFCVNHGDRIAQMVIARYEHAELQEVEELSRTPRGSGGFGHTGV

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=143, Percent_Identity=41.958041958042, Blast_Score=113, Evalue=6e-26,
Organism=Homo sapiens, GI4503423, Length=143, Percent_Identity=41.958041958042, Blast_Score=113, Evalue=7e-26,
Organism=Homo sapiens, GI70906441, Length=143, Percent_Identity=41.958041958042, Blast_Score=112, Evalue=2e-25,
Organism=Escherichia coli, GI1790071, Length=138, Percent_Identity=44.2028985507246, Blast_Score=122, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71988561, Length=143, Percent_Identity=41.958041958042, Blast_Score=115, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6319729, Length=134, Percent_Identity=38.0597014925373, Blast_Score=92, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19921126, Length=142, Percent_Identity=38.0281690140845, Blast_Score=96, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24583610, Length=140, Percent_Identity=38.5714285714286, Blast_Score=96, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_PROA2 (B4S912)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002016196.1
- ProteinModelPortal:   B4S912
- SMR:   B4S912
- GeneID:   6460336
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_1529
- HOGENOM:   HBG436079
- OMA:   HGIALVN
- ProtClustDB:   CLSK637750
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15684; Mature: 15684

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: BINDING 78-78 BINDING 92-92

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVNIVRVNKNAILPRYATQHAAGMDIAACLDVPLTIQPSETVLVPTGLAVELPEGYEA
CEEEEEEEECCCCCCCCCCCCCCCCCCEEHHCCCCEEECCCCEEEEECCCEEECCCCCCE
QLRPRSGLALKYAISLPNAPATIDADYRGEVKVILVNLGQEPFCVNHGDRIAQMVIARYE
EECCCCCCEEEEEEECCCCCEEEECCCCCCEEEEEEECCCCCEEECCCHHHHHHHHHHCC
HAELQEVEELSRTPRGSGGFGHTGV
CHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MLKVNIVRVNKNAILPRYATQHAAGMDIAACLDVPLTIQPSETVLVPTGLAVELPEGYEA
CEEEEEEEECCCCCCCCCCCCCCCCCCEEHHCCCCEEECCCCEEEEECCCEEECCCCCCE
QLRPRSGLALKYAISLPNAPATIDADYRGEVKVILVNLGQEPFCVNHGDRIAQMVIARYE
EECCCCCCEEEEEEECCCCCEEEECCCCCCEEEEEEECCCCCEEECCCHHHHHHHHHHCC
HAELQEVEELSRTPRGSGGFGHTGV
CHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA