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The map label for this gene is oprM [H]

Identifier: 194334206

GI number: 194334206

Start: 1517253

End: 1518680

Strand: Reverse

Name: oprM [H]

Synonym: Paes_1398

Alternate gene names: 194334206

Gene position: 1518680-1517253 (Counterclockwise)

Preceding gene: 194334207

Following gene: 194334205

Centisome position: 60.43

GC content: 54.41

Gene sequence:

>1428_bases
ATGAAACGAGATACAATGAACATATCGGTTACACAGCGCATTACTCTGATTGCTTTGATCGCATCGCTTTCTTCATGCAG
TATGAGCGGCAAATATATACAGCCGGAGACAGGCGTTCCATCCTCTTTCAGAGGAGAACAAAGCGCTCAGCAACACCCTC
TTCACTCCTCATCAACGATAGGACGCCTTTCCTACAGGACTTTTTTTCAGGATCCAGCGCTGCTTGTGCTCATTGAATCT
GCTATAGCGCATAACAGTGATCTTCAACTGGCGATGAAGAATATCGACTATGCATCGCTTACTCTCCGTCAGGCCGGGCT
TTCGGGGCTTCCCGGATTTGGTCTGCAGGGGAGCGCCAGTTCATCGGGAACCTCTGACTACGGGAGTAGCCCGCTTTCCG
ATGGAGAAGAGAGCGTCGAGACGTTCAGCGCATCGGCTTACGCCTCATGGGAACTTGATATCTGGGGTAAAATCCGCAGC
CGCAAAAAGTCGGCGCTTGCAGGCTATCTTGAATCGGTCGAGGCAGGAAAAGCGGTGCAGACCCGCCTTGTGGCTTCTGT
GGCGGAAGGATACTCTACGCTGCAGATGCTCGATACACAGTATGCGGTAACCGCTCAAAACCTGGAGCTTGCCGCTACGA
CGCTTTCCATGATGCGTCTCCAGTATGATGCGGGGCTGGTGACCTCACTGGCGGTTGACCGGCAGGAGGCATTGAAGCTC
GATGCCGAGGCCGGTCTGGCAAAGATCGAGCAGCAGATCGCGCTTCAGGAAAACGCGTTGAGCGTTCTGTGCGGAAGGAT
GCCTGAAGGTATTGAGCGTGGCGCTGTACGTCCTATGCAGAATCGCTTTTCCGCTGGCATTCCTGCCGCACTGCTTTCAA
ACAGGCCGGATGTCAAGGCTGCCGAGCTTGCGTTGATGAAAGCGCATGCAGAGACCGGAGTCGCAGCGGCAAACCTGTAT
CCGTCACTGACAATCAGTGCTGAAGCAGGCTCTGAGGCACTCAAGGCAAGCGACTGGTTTACGTTTCCCGGCTCATTGTT
CAGTTTTGTTCAGGGCGCTGTTCTGCAGCCGATTTTTCAGCAGGGAACATTGAGGACAGCCTACAAGCAGTCGCAGGTCG
TGCGTGAAGAGGAGGAGATACGTTTTCGCCAGGCGGTTCTCGATGCGGTCAGAGAGGTTTCTGACGCTCTTGTGACGATT
GATAAGCTCGATGTTCAGCAACGGGCAGCAGAAAAGCAGGTCGCTCTTCTTCAGCAGTCGGTTACCAATGCCGGTCTTCT
GTTTCAGGGCGGTATGGCTGATTACCTTGACGTTATTTCTGCTCAGTCCGCAGCCCTCGATGCGGAACTTTCCCTTTCAG
ATATTCGTCGTCAGCAGCGCAATGCGGTGACGGAGCTCTACCGGGCAGCGGGAGGGGGCTGGCAGTAG

Upstream 100 bases:

>100_bases
AGGAGAAGATCAGCGGGCCTGCGCGCGGGTTTGATGATGATCCCCTTCAGGAGGAGTGATGCCAGTGCGATATGAATCGG
CAGAGAGGAATTGATAACGG

Downstream 100 bases:

>100_bases
TGAGGCAACGCAGATAGACAGGAATATTTTGAAAAGCATTCGGGAAAACAGCATCTTTGCATACAATGCCTTTTCTGGTA
TTGTTCTGTCTTCTTTGTGA

Product: NodT family RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKRDTMNISVTQRITLIALIASLSSCSMSGKYIQPETGVPSSFRGEQSAQQHPLHSSSTIGRLSYRTFFQDPALLVLIES
AIAHNSDLQLAMKNIDYASLTLRQAGLSGLPGFGLQGSASSSGTSDYGSSPLSDGEESVETFSASAYASWELDIWGKIRS
RKKSALAGYLESVEAGKAVQTRLVASVAEGYSTLQMLDTQYAVTAQNLELAATTLSMMRLQYDAGLVTSLAVDRQEALKL
DAEAGLAKIEQQIALQENALSVLCGRMPEGIERGAVRPMQNRFSAGIPAALLSNRPDVKAAELALMKAHAETGVAAANLY
PSLTISAEAGSEALKASDWFTFPGSLFSFVQGAVLQPIFQQGTLRTAYKQSQVVREEEEIRFRQAVLDAVREVSDALVTI
DKLDVQQRAAEKQVALLQQSVTNAGLLFQGGMADYLDVISAQSAALDAELSLSDIRRQQRNAVTELYRAAGGGWQ

Sequences:

>Translated_475_residues
MKRDTMNISVTQRITLIALIASLSSCSMSGKYIQPETGVPSSFRGEQSAQQHPLHSSSTIGRLSYRTFFQDPALLVLIES
AIAHNSDLQLAMKNIDYASLTLRQAGLSGLPGFGLQGSASSSGTSDYGSSPLSDGEESVETFSASAYASWELDIWGKIRS
RKKSALAGYLESVEAGKAVQTRLVASVAEGYSTLQMLDTQYAVTAQNLELAATTLSMMRLQYDAGLVTSLAVDRQEALKL
DAEAGLAKIEQQIALQENALSVLCGRMPEGIERGAVRPMQNRFSAGIPAALLSNRPDVKAAELALMKAHAETGVAAANLY
PSLTISAEAGSEALKASDWFTFPGSLFSFVQGAVLQPIFQQGTLRTAYKQSQVVREEEEIRFRQAVLDAVREVSDALVTI
DKLDVQQRAAEKQVALLQQSVTNAGLLFQGGMADYLDVISAQSAALDAELSLSDIRRQQRNAVTELYRAAGGGWQ
>Mature_475_residues
MKRDTMNISVTQRITLIALIASLSSCSMSGKYIQPETGVPSSFRGEQSAQQHPLHSSSTIGRLSYRTFFQDPALLVLIES
AIAHNSDLQLAMKNIDYASLTLRQAGLSGLPGFGLQGSASSSGTSDYGSSPLSDGEESVETFSASAYASWELDIWGKIRS
RKKSALAGYLESVEAGKAVQTRLVASVAEGYSTLQMLDTQYAVTAQNLELAATTLSMMRLQYDAGLVTSLAVDRQEALKL
DAEAGLAKIEQQIALQENALSVLCGRMPEGIERGAVRPMQNRFSAGIPAALLSNRPDVKAAELALMKAHAETGVAAANLY
PSLTISAEAGSEALKASDWFTFPGSLFSFVQGAVLQPIFQQGTLRTAYKQSQVVREEEEIRFRQAVLDAVREVSDALVTI
DKLDVQQRAAEKQVALLQQSVTNAGLLFQGGMADYLDVISAQSAALDAELSLSDIRRQQRNAVTELYRAAGGGWQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=455, Percent_Identity=26.1538461538462, Blast_Score=158, Evalue=8e-40,
Organism=Escherichia coli, GI2367348, Length=350, Percent_Identity=25.4285714285714, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51032; Mature: 51032

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRDTMNISVTQRITLIALIASLSSCSMSGKYIQPETGVPSSFRGEQSAQQHPLHSSSTI
CCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCHHHHCCCCCCCCH
GRLSYRTFFQDPALLVLIESAIAHNSDLQLAMKNIDYASLTLRQAGLSGLPGFGLQGSAS
HHHHHHHHHCCCHHEEHHHHHHCCCCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCC
SSGTSDYGSSPLSDGEESVETFSASAYASWELDIWGKIRSRKKSALAGYLESVEAGKAVQ
CCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHH
TRLVASVAEGYSTLQMLDTQYAVTAQNLELAATTLSMMRLQYDAGLVTSLAVDRQEALKL
HHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHH
DAEAGLAKIEQQIALQENALSVLCGRMPEGIERGAVRPMQNRFSAGIPAALLSNRPDVKA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHCCCCCHHHHCCCCCCHH
AELALMKAHAETGVAAANLYPSLTISAEAGSEALKASDWFTFPGSLFSFVQGAVLQPIFQ
HHHHHHHHHHHCCCCHHCCCCCEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
QGTLRTAYKQSQVVREEEEIRFRQAVLDAVREVSDALVTIDKLDVQQRAAEKQVALLQQS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTNAGLLFQGGMADYLDVISAQSAALDAELSLSDIRRQQRNAVTELYRAAGGGWQ
HCCCCEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKRDTMNISVTQRITLIALIASLSSCSMSGKYIQPETGVPSSFRGEQSAQQHPLHSSSTI
CCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCHHHHCCCCCCCCH
GRLSYRTFFQDPALLVLIESAIAHNSDLQLAMKNIDYASLTLRQAGLSGLPGFGLQGSAS
HHHHHHHHHCCCHHEEHHHHHHCCCCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCC
SSGTSDYGSSPLSDGEESVETFSASAYASWELDIWGKIRSRKKSALAGYLESVEAGKAVQ
CCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHH
TRLVASVAEGYSTLQMLDTQYAVTAQNLELAATTLSMMRLQYDAGLVTSLAVDRQEALKL
HHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHH
DAEAGLAKIEQQIALQENALSVLCGRMPEGIERGAVRPMQNRFSAGIPAALLSNRPDVKA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHCCCCCHHHHCCCCCCHH
AELALMKAHAETGVAAANLYPSLTISAEAGSEALKASDWFTFPGSLFSFVQGAVLQPIFQ
HHHHHHHHHHHCCCCHHCCCCCEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
QGTLRTAYKQSQVVREEEEIRFRQAVLDAVREVSDALVTIDKLDVQQRAAEKQVALLQQS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTNAGLLFQGGMADYLDVISAQSAALDAELSLSDIRRQQRNAVTELYRAAGGGWQ
HCCCCEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]