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The map label for this gene is rlmB [H]

Identifier: 194334189

GI number: 194334189

Start: 1502839

End: 1503384

Strand: Reverse

Name: rlmB [H]

Synonym: Paes_1381

Alternate gene names: 194334189

Gene position: 1503384-1502839 (Counterclockwise)

Preceding gene: 194334190

Following gene: 194334188

Centisome position: 59.83

GC content: 49.27

Gene sequence:

>546_bases
ATGAAGGGATTTTCCAAGCTTAACGGTGATGAAATGGGCCGTTTGGGCGTTGAAGAATACAACGCTGCTGAAAAGCACCC
GGTGACGATTATTCTTCACAATATCCGGAGCATGTATAATGTCGGTTCAATTTTCAGGACGGCTGATGCGGCAGGGATAG
AGAAAGTGATCATCACCGGTTATACGGCGACTCCACCGCGCAAGGAGATCGATAAGACGGCTCTGGGTGCTCAGGAGACA
ATGGCGTGGGAGTATTTTGCGCATCCTCTGGAGGTGATTCCTGCTATGAAGGAGAAGGGGATTGCCATATGCGGGCTTGA
GATAACGACGAACAGTCGCGCGTATACCGATGTGGACCGGAGTGATTTTCCGCTGTGCCTGATTCTGGGGAACGAAGTTC
AGGGAATTGATGACGATATACTCGAGCAGTGTGATCATATTCTTGAAATCCCGCAGTTCGGCACAAAGCATTCATTGAAT
GTTTCGGTTGCAGCAGGGGTGGCTTTATTTGAAATGGTTAGAACTTTCCGGCAGGGCCTTTGTTGA

Upstream 100 bases:

>100_bases
ACGTTTCAGGTTACCGGCTGATGGAGAATTGTCTGCGTTTTAATATCGGGCTTGAGACAGAAAATGATCTTTTGCTTGAA
AAACTGCACCGCATGAAGGG

Downstream 100 bases:

>100_bases
TGCCGGAGCAAGGGGGGAATTGAATACGACAATGCTTCATTACAGAATTCACGAATTGGCAGAAGATGCCCCATGGGTTG
TCTTTGTTCATGGAGCGGGT

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MKGFSKLNGDEMGRLGVEEYNAAEKHPVTIILHNIRSMYNVGSIFRTADAAGIEKVIITGYTATPPRKEIDKTALGAQET
MAWEYFAHPLEVIPAMKEKGIAICGLEITTNSRAYTDVDRSDFPLCLILGNEVQGIDDDILEQCDHILEIPQFGTKHSLN
VSVAAGVALFEMVRTFRQGLC

Sequences:

>Translated_181_residues
MKGFSKLNGDEMGRLGVEEYNAAEKHPVTIILHNIRSMYNVGSIFRTADAAGIEKVIITGYTATPPRKEIDKTALGAQET
MAWEYFAHPLEVIPAMKEKGIAICGLEITTNSRAYTDVDRSDFPLCLILGNEVQGIDDDILEQCDHILEIPQFGTKHSLN
VSVAAGVALFEMVRTFRQGLC
>Mature_181_residues
MKGFSKLNGDEMGRLGVEEYNAAEKHPVTIILHNIRSMYNVGSIFRTADAAGIEKVIITGYTATPPRKEIDKTALGAQET
MAWEYFAHPLEVIPAMKEKGIAICGLEITTNSRAYTDVDRSDFPLCLILGNEVQGIDDDILEQCDHILEIPQFGTKHSLN
VSVAAGVALFEMVRTFRQGLC

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=153, Percent_Identity=31.3725490196078, Blast_Score=76, Evalue=1e-15,
Organism=Escherichia coli, GI1790083, Length=156, Percent_Identity=28.8461538461538, Blast_Score=68, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324775, Length=167, Percent_Identity=28.1437125748503, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 19960; Mature: 19960

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGFSKLNGDEMGRLGVEEYNAAEKHPVTIILHNIRSMYNVGSIFRTADAAGIEKVIITG
CCCCCCCCCCHHCCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
YTATPPRKEIDKTALGAQETMAWEYFAHPLEVIPAMKEKGIAICGLEITTNSRAYTDVDR
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCEEECCCC
SDFPLCLILGNEVQGIDDDILEQCDHILEIPQFGTKHSLNVSVAAGVALFEMVRTFRQGL
CCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
C
C
>Mature Secondary Structure
MKGFSKLNGDEMGRLGVEEYNAAEKHPVTIILHNIRSMYNVGSIFRTADAAGIEKVIITG
CCCCCCCCCCHHCCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
YTATPPRKEIDKTALGAQETMAWEYFAHPLEVIPAMKEKGIAICGLEITTNSRAYTDVDR
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCEEECCCC
SDFPLCLILGNEVQGIDDDILEQCDHILEIPQFGTKHSLNVSVAAGVALFEMVRTFRQGL
CCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
C
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]